Immunogenicity of formaldehyde and binary ethylenimine inactivated infectious bursal disease virus in broiler chicks

Habib, Md. Ahasan; Hussaın, Iftikhar; Irshad, Hamid; Yang, Zong-zhao; Shuai, Jiangbing; Chen, Ning

doi:10.1631/jzus.2006.b0660

Cited by 14 publications

(7 citation statements)

References 16 publications

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“…Now more and more report indicated that envelope proteins, especially VP28, could offer shrimp protection against WSSV infection [22][23][24][25]. In this investigation we found that the protection efficacy of inactivated WSSV is dependent on the integrity of envelope proteins as previous report in the vaccination trial of veterinary virus [30,32,39,40]. We also found that the protection will decrease along with day after last vaccination like previous reports [23,27].…”

Section: Disscussionsupporting

confidence: 84%

Protection of Procambarus clarkii against white spot syndrome virus using inactivated WSSV

Zhu

Miao

et al. 2009

Fish & Shellfish Immunology

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Section: Disscussionsupporting

confidence: 84%

Protection of Procambarus clarkii against white spot syndrome virus using inactivated WSSV

Zhu

Miao

et al. 2009

Fish & Shellfish Immunology

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“…These results agreed with the results of Amal (2001) which found that evaluation of the chick´s immune response to the locally prepared inactivated IBDV vaccine adjuvant with Montanide oil ISA 70 showed the highest antibody titers at 4th week post vaccination and continued to be protective for the subsequent 12 weeks, using SNT and ELISA. These results agree with that of Habib et al, (2006), who showed that on the basis of humoral immune response, the inactivated IBDV vaccines were immunogenic with increased in antibody titers in all inoculated groups 2 weeks post inoculation. These results agreed also with the facts showed that the humoral immune response plays the principal role in defense against vvIBDV (Lukert & Saif, 1997).…”

Section: Discussionsupporting

confidence: 92%

“…The completion of virus inactivation was tested by its inoculation in 11 days old SPF-ECEs on CAM that showed no pathological lesions and / or deaths of inoculated embryos, compared with that of the control one. This result disagreed with the studies used formalin 0.01% for IBDV inactivation that showed complete virus inactivation after 18 hrs and 24 hrs respectively by Amal 2001and Habib et al, (2006).…”

Section: Discussioncontrasting

confidence: 80%

Evaluation of an inactivated infectious bursal disease virus vaccine prepared using a local isolate from Egypt

El-Bagoury

El-Habbaa

Elsayed

et al. 2015

Benha Veterinary Medical Journal

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Inactivated infectious bursal disease virus (IBDV) vaccine was prepared using a local isolate from Egypt with Montanide ISA70VG as oil adjuvant. The prepared vaccine was evaluated in comparison with an inactivated imported vaccine. Chicks vaccinated with either prepared or imported vaccines showed high serum antibody titers from the 3rd week post vaccination and reached the highest titer at the 4th week post vaccination using SNT and ELISA. Duration of suitable immune response prolonged to 14 weeks post vaccination for the prepared vaccine and 16 weeks post vaccination for the imported vaccine. Both prepared and imported vaccines showed 100% protection in vaccinated chicks challenged with the very virulent IBDV 21 days post vaccination with no clinical signs or lesions on examination. It was concluded that inactivated vaccine prepared from local isolated IBDV strain was safe, potent and immunogenic in young chicks.

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“…BEI reacts with viral nucleic acids while maintaining the conformation and accessibility of the epitope to a much greater degree than formaldehyde and BPL [15]. Various viruses have been successfully inactivated using BEI for vaccine production, such as infectious bursal disease viruses [16], foot and mouth disease viruses [17], rabies virus [18-20], and Japanese encephalitis viruses [21].…”

Section: Introductionmentioning

confidence: 99%

The efficacy of binary ethylenimine-inactivated vaccines of Gianyar-1/AK/2014 virulent strain in protecting chickens against Tabanan-1/ARP/2017 virulent Newcastle disease virus isolates

2019

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Aim: This study aimed to prepare binary ethylenimine (BEI)-inactivated virulent Newcastle disease virus (NDV) vaccine and to examine their ability to induce a protective antibody response in commercial chickens. Materials and Methods: A virulent NDV field isolate Gianyar-1/AK/2014 was propagated in chicken-embryonated eggs and was then inactivated with BEI at a concentration of 4 mM. Three groups of chickens with low-level (2 log2 hemagglutination inhibition [HI] units) maternally derived antibodies against NDV were then immunized with the BEI-inactivated vaccine. A commercial live vaccine (LaSota strain) was used as positive control, and phosphate-buffered saline (PBS) was used as negative control. A challenge experiment with a virulent NDV of Tabanan-1/ARP/2017 was performed at 3 weeks post-vaccination. Results: At 2 weeks post-immunization, the mean titers of antibodies against NDV in serum samples of chickens immunized with 0.2 mL of BEI-inactivated NDV (Group I), with live commercial NDV vaccine (Group II) and with PBS (Group III) were 3±0.94 log2 HI units, 4.9±0.99 log2 HI unit, and 0.0±0.0 HI units, respectively. At week 3 post-immunization, the mean titers of the antibodies for the three groups were 5±1.09 log2 HI units, 6.9±0.32 log2 HI units, and 0.00 HI units, respectively. The antibody titer induced by inactivated NDV Gianyar-1/AK/2014 isolates examined at 2 and 3 weeks post-vaccination was still at a significantly (p<0.01) lower level as compared to those induced by commercial life vaccine. However, the challenge test with virulent NDV of Tabanan 1/ARP/2017 isolates showed that all immunized chickens (Group I and II) survived without exhibiting any clinical sign post-challenge with the protection rates of 100%, whereas all chickens injected with PBS (Group III) died with clinical signs of ND. Conclusion: This finding shows that the BEI-inactivated vaccines prepared using virulent NDV of Gianyar-1/AK/2014 strain was able to induce protective antibody response in chickens but still at a lower level than those induce by commercial live NDV vaccine.

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Immunogenicity of formaldehyde and binary ethylenimine inactivated infectious bursal disease virus in broiler chicks

Cited by 14 publications

References 16 publications

Protection of Procambarus clarkii against white spot syndrome virus using inactivated WSSV

Protection of Procambarus clarkii against white spot syndrome virus using inactivated WSSV

Evaluation of an inactivated infectious bursal disease virus vaccine prepared using a local isolate from Egypt

The efficacy of binary ethylenimine-inactivated vaccines of Gianyar-1/AK/2014 virulent strain in protecting chickens against Tabanan-1/ARP/2017 virulent Newcastle disease virus isolates

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