Immunoglobulin A as a Key Immunological Molecular Signature of Post-COVID-19 Conditions

Sousa, Graziele F.; Carpes, Raphael M.; Silva, Carina A. O.; Pereira, Marcela E. P.; Silva, Amanda C. V. F.; Coelho, Viktoria A. G. S.; Costa, Evenilton P.; Mury, Flávia B.; Gestinari, Raquel S.; Souza-Menezes, Jackson; Leal-da-Silva, Manuela; Nepomuceno-Silva, José L.; Tanuri, Amilcar; Ferreira-Júnior, Orlando C.; Monteiro-de-Barros, Cintia

doi:10.3390/v15071545

Cited by 1 publication

(2 citation statements)

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“…The S-UFRJ ELISA, as described by Alvim et al (2022) , was performed with the trimeric SARS-COV-2 Spike glycoprotein, kindly provided by the Laboratory of Cell Culture Engineering (LECC) from the Federal University of Rio de Janeiro (UFRJ). The protocols for semi-quantitative analysis of IgA, IgM, and IgG were previously described by Sousa et al (2023) . This analysis was performed using a Multiscan Sky microplate reader (Thermo Scientific) at 450 nm and results were expressed as the optical density (OD) or OD/cutoff ratio of the sample.…”

Section: Methodsmentioning

confidence: 99%

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Genomic surveillance and serological profile of SARS-CoV-2 variants circulating in Macaé and nearby cities, southeastern Brazil

da Silva,

Silva,

de Sousa

et al. 2024

Front. Microbiol.

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IntroductionA characteristic of the COVID-19 pandemic has been the sequential emergence and global dissemination of SARS-CoV-2 variants, noted for their enhanced transmission efficiency. These variants with mutations in the Spike glycoprotein (S-glycoprotein), which interacts with ACE2 receptors in human cells is critical for infection, affects the transmissibility of the virus, which is a matter of great concern for public health.ObjectiveThis research analyses the effects these variants on a cohort of vaccinated and naturally infected individuals from the cities of Macaé-RJ, Rio das Ostras-RJ, and Campos dos Goytacazes-RJ, Brazil, from March 2021 to March 2023.MethodsThis investigation encompasses the Alpha (B.1.1.7), Gamma (P.1), Delta (B.1.617.2, B.1.671.3), and Omicron (BQ.1, BQ.1.1 sublines, and BF.7) variants, focusing on their genomic surveillance and implications for the disease’s epidemiology. The experimental analysis included a control group (vaccinated and uninfected subjects), and an infected group (post-vaccinated subjects). Samples from nasopharyngeal swabs underwent viral detection via RT-qPCR for diagnosis confirmation. RNase H-dependent RT-qPCR (rhAmp-PCR) and third-generation sequencing were used to detect SARS-CoV-2 variants. Anti-S-glycoprotein immunoglobulins were also evaluated for vaccinated infected and noninfected volunteers. Symptoms from infected individuals were compiled in order to reveal patterns of clinical signs associated with viral infection.ResultsThe study included 289 participants, with infections identified by Gamma (n = 44), Delta (n = 189), and Omicron (n = 56) variants. The prevalent symptoms among the naturally infected participants were cough, fever, sore throat, headache, and runny nose. For Omicron, cognitive symptoms such as memory loss and concentration issues were reported. Interestingly, the infected vaccinated group had higher anti-S-glycoprotein IgM production (n = 28, 0.2833 ± 0.09768 OD) compared to the uninfected vaccinated group (n = 14, 0.1035 ± 0.03625 OD). Conversely, anti-S-glycoprotein IgG production was higher in the control group (n = 12, 1.770 ± 0.1393 OD) than in the infected vaccinated group (n = 26, 1.391 ± 0.1563 OD).ConclusionThis comprehensive study enables monitoring of predominant variants and their correlation with clinical cases, providing valuable insights for public health. Our research group continues to survey circulating variants, contributing to the global understanding of the pandemic.

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Section: Methodsmentioning

confidence: 99%

“…Blood samples were obtained via peripheral venipuncture, collected into 4 mL EDTA K3 tubes (Firstlab). Plasma was subsequently isolated through centrifugation at 3000 g using a Daiki DT-5000 for 10 min and preserved at −20°C for future analysis ( Sousa et al, 2023 ).…”

Section: Methodsmentioning

confidence: 99%