Immunoglobulin Isotype Modulation after Administration of IL-12

Cleave, Victor Van; Wolf, Stan; Murray, Kristin S.; Wiencis, Anna; Ketchum, Mara A.; Bliss, Judy L.; Haire, T; Resmini, Christine; Maylor, Rich; Alderman, Ed

doi:10.1007/978-1-4615-1891-4_6

Cited by 8 publications

(2 citation statements)

References 17 publications

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“…The proportion of IgG antibodies of the cytophilic subclasses (IgG1 and IgG3) increases with age among children (Gregorek et al, 1994) and adults (Powers, 1994;Yachie et al, 1995). IgG-subclass ratios are apparently regulated by cytokines; IL-2 and IL-12 induce IgG2 whereas IL-6 and IL-10 (Th2-type cytokines) induce IgG1, IgG3 and IgG4 (Briere et al, 1994;Kawano et al, 1994Kawano et al, , 1995Buchanan et al, 1995;Lorenz et al, 1995;Van Cleave et al, 1995;Kawano and Noma, 1996). Mice challenged with P. chabaudi chabaudi produced IgG2 when the immune response was dominated by Th1 cytokines, and later switched to Th2 cytokines and predominance of IgG1 (D' Imperio et al, 1996).…”

Section: Tionsmentioning

confidence: 99%

Age dependent characteristics of protection v. susceptibility to Plasmodium falciparum

Jk¹

1998

Annals of Tropical Medicine And Parasitology

192

141

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Section: Tionsmentioning

confidence: 99%

Age dependent characteristics of protection v. susceptibility to Plasmodium falciparum

Jk¹

1998

Annals of Tropical Medicine And Parasitology

192

141

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“…Because IL‐12 and IFN‐γ comprise a paracrine feedback system for increasing the levels of IFN‐γ production by NK cells following activation of Mφ, it is likely that antigens that elicit IL‐12 release from Mφ are powerful adjuvants for stimulating a Th1 response. Both IFN‐γ and IL‐12 have been studied for their association with Th1 cell development leading to the production of IgG2a antibodies 29–31 …”

Section: Discussionmentioning

confidence: 99%

Natural killer cell‐dependent immunoglobulin G2a anti‐bovine serum albumin (BSA) response elicited by high molecular weight dextran–BSA conjugates associated with dextran‐mediated macrophage–natural killer cell interaction

2000

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SUMMARYThe roles of the interferon-c (IFN-c) and interleukin-12 (IL-12) produced during natural killer (NK) cell interaction with macrophages (Mw) were investigated as the basis for the induction of immunoglobulin G2a (IgG2a) anti-bovine serum albumin (BSA) responses by high molecular weight dextran conjugated to BSA (HMW-DEX±BSA). BALB/c mice immunized with HMW-DEX±BSA produced signi®cantly higher levels of both IgG1 and IgG2a anti-BSA than did mice immunized with BSA alone. Both IgG1 and IgG2a anti-BSA levels were higher in mice immunized with BSA conjugated to dextran of molecular weight (MW) 5 000 000±40 000 000 compared with dextran of MW 10 000±60 000. The enhancement of anti-BSA IgG2a levels but not of anti-BSA IgG1 levels was inhibited when free BSA was added to the HMW-DEX±BSA conjugate. NK cell depletion during HMW-DEX±BSA immunization of mice resulted in signi®cantly lower anti-BSA IgG2a levels without affecting anti-BSA IgG1 levels. Naive splenocytes or Mw+NK cell co-cultures incubated with HMW-DEX or HMW-DEX±BSA produced higher IFN-c levels than splenocytes or co-cultures incubated with BSA alone. HMW-DEX stimulated both IFN-c and IL-12 production by Mw+NK cell co-cultures in a dose-dependent manner. DEX-induced IFN-c production by NK cells was dependent upon the presence of IL-12, and IL-12 production by Mw was dependent upon the presence of IFN-c in these co-cultures. Both Mw and NK cells bound DEX to their surfaces. These data demonstrate that BSA linked to HMW-DEX enhanced both T-helper-1-and T-helper-2-associated antibody responses to BSA. The results also indicate an IL-12-dependent positive feedback interaction between NK cells and Mw that supports a NK cell/ IFN-c-dependent mechanism for enhancement of anti-BSA IgG2a antibody responses in mice immunized with HMW-DEX±BSA protein conjugates.

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