Immunohistochemical and electron microscopic studies on epithelial tumor cells of cystadenolymphomas.

Abstract

Eosinophilic epithelial tumor cells of cystadenolymphomas consisted of two different cell types as determined by electron microscopy: light tumor cells, located on the apical side and containing mitochondria without cristae, and dark tumor cell, located on the basal side and containing mitochondria with large and tightly packed cristae and also lysosomes. As found immunohistochemically, keratins and other proteins were expressed differently in light (apical) and dark (basal) tumor cells. The dark tumor cells were positively stained by monoclonal antibody PKK1(nos. 19, 18, 8; Carnoy's-fixed sections), K8.12 (nos. 16, 13; formalin-fixed sections), K4.62 (no. 19) and by monoclonals 115 F5 and 115 D8 against epithelial membrane antigens and by monoclonal KM231 against a human gastric cancer antigen. The immunohistochemical localization and pattern of staining is compared between dark tumor cells of cystadenolymphomas and ductal basal cells of the normal salivary gland.

Warthin's Tumour of the Parotid Glands, Adenomatous Hyperplasia and Oncocytic Adenomatous Hyperplasia of the Intranodal Heterotopic Salivary Glands: A Comparative Immunohistochemical Assessment.

“…In prenatally developing salivary glands, ductal basal cells have been found to be similar to those found in the adult glands with respect to the expression of cytokeratins detected by antibodies K8.12 and PKK1 which appears relatively early during the differentiation of larger ducts [21]. At the ultrastructual level, no significant difference has been noted between the luminal and basal cells of Warthin's tumours, but basal tumour cells have been shown to closely resemble the ductal basal cells of normal parotid glands [41]. In the present study, the cytokeratin expression in the ductal cells, AH and OAH of the intranodal HSGT was found to mimic the epithelial components of Warthin's tumours.…”

“…In the ductal epithelium of normal salivary glands, the antibodies K8.12, PKK1 and KL1 were reactive with the luminal and basal cells in the intercalated, striated and excretory ducts with different reaction intensities. The immunoreactivity of antibody K8.12 was predominantly present in the basal cells, while that of antibodies PKK1 and KL1 was equally present in the luminal and basal cells [3,8,29,39,41]. In prenatally developing salivary glands, ductal basal cells have been found to be similar to those found in the adult glands with respect to the expression of cytokeratins detected by antibodies K8.12 and PKK1 which appears relatively early during the differentiation of larger ducts [21].…”

“…The cytokeratins detected by antibodies K8.12, PKK1 and KL1 have been consistently demonstrated in normal and neoplastic lesions of the salivary glands [3,8,12,23,39,41]. In the ductal epithelium of normal salivary glands, the antibodies K8.12, PKK1 and KL1 were reactive with the luminal and basal cells in the intercalated, striated and excretory ducts with different reaction intensities.…”

“…Ultrastructural and immunohistochemical studies have shown remarkable similarities between the ductal cells in normal salivary glands and the epithelial tumour cells of Warthin's tumour [41]. The characteristics of intranodal HSGT, however, remain largely unknown .…”