Immunohistochemical study of sustentacular cells in adrenal medulla of neonatal and adult rats using an antibody against S-100 protein

Ahmed, Aly M.

doi:10.5603/fm.a2016.0066

Cited by 5 publications

(7 citation statements)

References 20 publications

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“…Sustentacular cells in adult are mainly located in the noradrenergic region of adrenal medulla (Suzuki and Kachi, 1994). These cells form sheet-like structures partially enveloping groups of noradrenergic chromaffin cells (Ahmed, 2017, Pakkarato, et al, 2015. The function of sustentacular cells in the adrenal is still unclear, however it is believed that they play a role in supporting and regulating catecholamine secretion (Ahmed, 2017, Pakkarato, et al, 2015, Rodriguez, et al, 2007.…”

Section: Origin Of Sympathetic Gliamentioning

confidence: 99%

From proliferation to target innervation: signaling molecules that direct sympathetic nervous system development

2017

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The sympathetic division of the autonomic nervous system includes a variety of cells including neurons, endocrine cells and glial cells. A recent study (Furlan et al. 2017) has revised thinking about the developmental origin of these cells. It now appears that sympathetic neurons and chromaffin cells of the adrenal medulla do not have an immediate common ancestor in the form a "sympathoadrenal cell", as has been long believed. Instead, chromaffin cells arise from Schwann cell precursors. This review integrates the new findings with the expanding body of knowledge on the signalling pathways and transcription factors that regulate the origin of cells of the sympathetic division of the autonomic nervous system.

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Section: Origin Of Sympathetic Gliamentioning

confidence: 99%

From proliferation to target innervation: signaling molecules that direct sympathetic nervous system development

2017

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“…Very little is known about sustentacular cells, especially their functional role and how they impact chromaffin cell stimulus-secretion coupling. In the rat, sustentacular cells differ in their number, size and shape between neonate and adult (6). The stellate shape, associated with numerous slender and long branched cytoplasmic processes observed in the adult, suggests close appositions with chromaffin cells and subsequent functional interactions.…”

Section: Anatomy and Developmentmentioning

confidence: 98%

“…Endocrine glands and particularly pituitary networks can modify their organization and store long-term memories of increased output and enhance function on repeated challenge. 6. Understanding the importance of endocrine gland cell organization on the regulation of circulating hormone concentrations provides new understanding of a range of endocrine axes defects and targets for novel therapies.…”

Section: Didactic Synopsismentioning

confidence: 99%

Cell Networks in Endocrine/Neuroendocrine Gland Function

Guérineau

Campos

Tissier

et al. 2022

Comprehensive Physiology

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Reproduction, growth, stress and metabolism are determined by endocrine/neuroendocrine systems that regulate circulating hormone concentrations. All these systems generate rhythms and changes in hormone pulsatility observed in a variety of pathophysiological states. Thus, the output of endocrine/neuroendocrine systems must be regulated within a narrow window of effective hormone concentrations but must also maintain a capacity for plasticity to respond to changing physiological demands. Remarkably most endocrinologists still have a 'textbook' view of endocrine gland organization which has emanated from 20 th century histological studies on thin 2D tissue sections. However, 21 st century technological advances, including indepth 3D imaging of specific cell types have vastly changed our knowledge. We now know that various levels of multi-cellular organization can be found across different glands, that organizational motifs can vary between species and can be modified to enhance or decrease hormonal release. This review focuses on how the organization of cells regulates hormone output using three endocrine/neuroendocrine glands that present different levels of organization and complexity: the adrenal medulla, with a single neuroendocrine cell type; the anterior pituitary, with multiple intermingled cell types; and the pancreas with multiple intermingled cell types organized into distinct functional units. We give an overview of recent methodologies that allow the study of the different components within endocrine systems, and particularly their temporal and spatial relationships. We believe the emerging findings about network organization, and its impact on hormone secretion, are crucial to understanding how homeostatic regulation of endocrine axes is carried out within endocrine organs themselves.

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“…These cells are present in the adrenal medulla [47,48] and thus likely dissociated together with ACC. Indeed, cell cultures derived from either wt or transgenic mice that were stained with antibodies against S100, a calcium-binding protein highly expressed by peripheral, neural crest-derived glia such as adrenal SGC [49,50], failed to show the presence of S100 + cells, which were always detected in adrenal slices (S2 Fig) . This result was important since SGC, as noted above, are derived from the neural crest similar to ACC and thus also express Sox10. In other words, SGC would be expected to express GCaMP6f in these cultures if they adhered and survived.…”

Section: Characteristics Of Acc Cultures From Wt Sox10-gcamp6f and Th...mentioning

confidence: 99%

High signal-to-noise imaging of spontaneous and 5 ns electric pulse-evoked Ca2+ signals in GCaMP6f-expressing adrenal chromaffin cells isolated from transgenic mice

et al. 2023

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In studies exploring the potential for nanosecond duration electric pulses to serve as a novel modality for neuromodulation, we found that a 5 ns pulse triggers an immediate rise in [Ca2+]i in isolated bovine adrenal chromaffin cells. To facilitate ongoing efforts to understand underlying mechanisms and to work toward carrying out investigations in cells in situ, we describe the suitability and advantages of using isolated murine adrenal chromaffin cells expressing, in a Cre-dependent manner, the genetically-encoded Ca2+indicator GCaMP6f. Initial experiments confirmed that Ca2+ responses evoked by a 5 ns pulse were similar between fluorescent Ca2+ indicator-loaded murine and bovine chromaffin cells, thereby establishing that 5 ns-elicited excitation of chromaffin cells occurs reproducibly across species. In GCaMP6f-expressing murine chromaffin cells, spontaneous Ca2+ activity as well as nicotinic receptor agonist- and 5 ns evoked-Ca2+ responses consistently displayed similar kinetic characteristics as those in dye-loaded cells but with two-twentyfold greater amplitudes and without photobleaching. The high signal-to-noise ratio of evoked Ca2+ responses as well as spontaneous Ca2+ activity was observed in cells derived from Sox10-Cre, conditional GCaMP6f mice or TH-Cre, conditional GCaMP6f mice, although the number of cells expressing GCaMP6f at sufficiently high levels for achieving high signal-to-noise ratios was greater in Sox10-Cre mice. As in bovine cells, Ca2+ responses elicited in murine GCaMP6f-expressing cells by a 5 ns pulse were mediated by the activation of voltage-gated Ca2+ channels but not tetrodotoxin-sensitive voltage-gated Na+ channels. We conclude that genetically targeting GCaMP6f expression to murine chromaffin cells represents a sensitive and valuable approach to investigate spontaneous, receptor agonist- and nanosecond electric pulse-induced Ca2+ responses in vitro. This approach will also facilitate future studies investigating the effects of ultrashort electric pulses on cells in ex vivo slices of adrenal gland, which will lay the foundation for using nanosecond electric pulses to stimulate neurosecretion in vivo.

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Immunohistochemical study of sustentacular cells in adrenal medulla of neonatal and adult rats using an antibody against S-100 protein

Abstract: (Folia Morphol 2017; 76, 2: 246-251)

Cited by 5 publications

References 20 publications

From proliferation to target innervation: signaling molecules that direct sympathetic nervous system development

From proliferation to target innervation: signaling molecules that direct sympathetic nervous system development

Cell Networks in Endocrine/Neuroendocrine Gland Function

High signal-to-noise imaging of spontaneous and 5 ns electric pulse-evoked Ca2+ signals in GCaMP6f-expressing adrenal chromaffin cells isolated from transgenic mice

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