Immunohistochemical study on proliferative activity of experimental cholesteatoma

Abstract

In this study, we induced canal ligation cholesteatoma using Mongolian gerbils and investigated the hyperproliferative nature of canal ligation cholesteatoma by using an immunohistochemical technique with proliferation markers (cytokeratin 13/16, PCNA, EGFR, thrombomodulin). Canal ligation cholesteatoma using Mongolian gerbils had a hyperproliferative character in the suprabasal cell layer similar to human cholesteatomas. This result will provide a good morphological basis for future cholesteatoma animal research concerning the pathogenesis of cholesteatoma.

Nucleoplasm staining patterns and cell cycle–associated expression of Ki-67 in middle ear cholesteatoma

“…In scientific papers, the proliferative activity of experimental middle ear cholesteatoma has been assessed with a variety of diagnostic experimental tools and different markers of proliferation [8,9]. In contemporary immunohistochemistry, the application of monoclonal antibodies against different nuclear antigens provides a valuable and simple technique for in situ evaluation of proliferative properties.…”

EGFR expression in acquired middle ear cholesteatoma in children and adults

“…Several authors have analyzed EGFR expression in cases of cholesteatoma [3,4,7,8,10,12,14,16,18]. Immunohistochemical methods have been used most frequently [3,7,8,12,14,16,18], and these are considered to be the gold standard for detecting this receptor.…”

“…In the studies involving IHQ methods to detect EGFR [3,12,14,16], the sample size ranged from six to 29 fragments of middle ear cholesteatoma. The study with the largest sample [18], comprising 50 cases, did not consider different age groups.…”

“…Immunohistochemical methods have been used most frequently [3,7,8,12,14,16,18], and these are considered to be the gold standard for detecting this receptor. The techniques of in situ hybridization [4,10] and ELISA [4] have also been used for this diagnosis.…”

“…Recently, several studies have described EGFR expression in cases of middle ear cholesteatoma [3,7,8,12,14,16,18].…”