Immunohistochemical techniques to compare primary vs. metastatic mucinous carcinoma of the skin

Abstract

In a small subset of mucinous carcinomas (20% in this series), positive labeling for CK5/6 indicated primary cutaneous tumor. Staining with p63 also favored primary over metastatic disease. Myoepithelial cell layers were not consistently identified to enable the identification of primary disease.

Quantitative expression study of four cytokeratins and p63 in squamous cell carcinoma of the tongue: suitability for sentinel node navigation surgery using one-step nucleic acid amplification

“…For antigen retrieval, sections were heated in 10 mmol/l sodium citrate buffer (pH 6.0) for 15 min at 121°C. Sections were incubated with diluted mouse monoclonal antibodies against p63 (4A4; 1:200 dilution; DAKO)12 13 as a primary antibody overnight at 4°C. Subsequently, after washing in phosphate-buffered saline, sections were incubated with secondary antibody (ACUITY Advanced Polymer Detection System; Covance, New Jersey, USA).…”

Atypical Carcinoid with Prominent Mucinous Stroma: A Hitherto Unreported Variant of Pulmonary Neuroendocrine Tumor

“…However, primary cutaneous mucinous carcinoma is generally negative for neuroendocrine markers and frequently express p63 and high-molecular-weight cytokeratins. [14] Finally, the absence of mediastinum involvement at imaging studies excluded the possibility of a thymic origin.…”

Cutaneous meningioma: a potential diagnostic pitfall in p63 positive cutaneous neoplasms

“…The immunoreagent p63 is commonly utilized in immunohistochemical panels when evaluating cutaneous spindle and epithelioid cell lesions and is generally considered a marker of epithelial neoplasms, including those of epidermal and adnexal origin. In practice, p63 expression is particularly useful in supporting the diagnosis of a primary adnexal carcinoma over metastatic adenocarcinoma and in distinguishing sarcomatoid squamous cell carcinoma from atypical fibroxanthoma …”