Immunologic analysis of sperm-immobilizing factor found in sera of women with unexplained sterility

Isojima, Shinzo; Li, Tien Shun; Ashitaka, Yoshio

doi:10.1016/0002-9378(68)90307-4

Cited by 388 publications

(82 citation statements)

References 10 publications

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“…The method used was that of Isojima, Li & Ashitaka (1968). Inactivated serum (0-25 ml) was added to fresh semen (0-025 ml) and guinea-pig comple¬ ment (0-05 ml), and sperm motility was expressed as a percentage after incu¬ bation for 1 hr at 37°C.…”

Section: Sperm Immobilization Testmentioning

confidence: 99%

A Comparison of Screening Tests for Anti-Sperm Activity in the Serum of Infertile Women

Jones¹,

Ing²,

Kaye³

1973

Reproduction

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Section: Sperm Immobilization Testmentioning

confidence: 99%

A Comparison of Screening Tests for Anti-Sperm Activity in the Serum of Infertile Women

Jones¹,

Ing²,

Kaye³

1973

Reproduction

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“…In the deoxycholate (DOC) sperm extract, the antisperm isoantiserum (ASA) recognized proteins of 100, 96, 92, 82, 53, 47, 43, and 29 kD, and the antitestis isoantiserum (ATA) recognized proteins of 100, 96, 92, 82, 43, and 29 kD. In the lithium diiodosalicylate (LIS) sperm extract, ASA reacted with four antigen bands of 92, 30, 14, and 6 kD, and ATA reacted with proteins of 94, 92, 91,23,17,7, and 6 kD. In the reduced sodium dodecyl sulfate (SDS) sperm extract, ASA recognized proteins of 95, 76, 51, 41, 33,30,28,26, and 22 kD; ATA showed a reaction only with proteins of 100, 98, and 95 kD, and anti-LIS/sperm isoantiserum (ALA) recognized three proteins of 98,81, and 65 kD.…”

mentioning

confidence: 99%

Isoantigenicity of Rabbit Sperm, Testis, and Their Extracts as Demonstrated by Western Blot Enzyme Immunobinding Procedure

Naz

Bradford

Menge

1984

Archives of Andrology

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The isoantigenicity of rabbit germ cells and their extracts was studied by using a Western blot enzyme immunobinding procedure after systemic and local intrauterine immunization. In the deoxycholate (DOC) sperm extract, the antisperm isoantiserum (ASA) recognized proteins of 100, 96, 92, 82, 53, 47, 43, and 29 kD, and the antitestis isoantiserum (ATA) recognized proteins of 100, 96, 92, 82, 43, and 29 kD. In the lithium diiodosalicylate (LIS) sperm extract, ASA reacted with four antigen bands of 92, 30, 14, and 6 kD, and ATA reacted with proteins of 94, 92, 91,23,17,7, and 6 kD. In the reduced sodium dodecyl sulfate (SDS) sperm extract, ASA recognized proteins of 95, 76, 51, 41, 33,30,28,26, and 22 kD; ATA showed a reaction only with proteins of 100, 98, and 95 kD, and anti-LIS/sperm isoantiserum (ALA) recognized three proteins of 98,81, and 65 kD.The IgA of the immune uterine fluid (IUF) from the LIS/sperm-immunized rabbits recognized a 14-kD antigen in the DOC conceptus extract, a 51-kD antigen in the DOC testis extract, and 53-, 49-, 31-, and 28-kD proteins in the reduced SDS-sperm pellet extract. The IgA of the IUF from the NP-4O/pellet-immunized rabbits showed two proteins of about 100 kD, plus ones of 56, 36,31,29, 19,17, and 14 kD in the DOC conceptus extract: proteins of 92, 70, 51, 29, and 14 kD in the DOC testis extract; and of 61, 57, 53, 49, 35,31, and 28 kD in the reduced SDS-sperm pellet extract.

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“…Sera were also collected from men (n = 3 ) who had undergone vasectomy, which will henceforth be referred to as vasectomized sera. The presence of antisperm antibodies in immunoinfertile and vasectomized sera was demonstrated by the sperm immobilization technique (SIT) [7], sperm agglutination technique (SAT) [4], enzymelinked immunosorbant assay (ELISA) [ I 11. and human sperni penetration of zona-free hamster oocytes (SPA) 11 11. Control sera (n = 2) were collected from 28-to 36-year-old fertile individuals (referred to as fertile control) who had sired at least one healthy child and were free of ASAs in SIT, SAT, ELISA, and SPA.…”

Section: Methodsmentioning

confidence: 99%

Effects of Human Antisperm Antibodies on Development of Preimplantation Embryos

Ahmad

Naz

1992

Archives of Andrology

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The effects of antisperm antibodies (ASAs) present in sera of immunoinfertile patients and vasectomized men were investigated on preimplantation embryonic development in mice. Of the nine immunoinfertile sera tested, two were effective in inhibiting blastulation rates of in vitro cultured murine 2-cell embryos (p < .05 to ,002). Similarly, sera from two of the three vasectomized men were capable of affecting early embryonic development in mice (p < .05 to .002). Specificities of the embryotoxic effects of ASAs were further confirmed by culturing embryos in the presence of affinity-purified monovalent Fab' antibodies isolated from these sera. Fab' antibodies from only one of the two immunoinfertile patients whose sera affected blastulation rates, and from one of the three vasectomized men were effective in influencing blastulation rates of in vitro cultured 2-cell murine embryos (p < .05 to .001), mainly due to an arrest of development at 2 to %cell and morula stages. In the Western blot procedure, none of the immunoinfertile Fab' antibodies recognized any specific band on blots of extracts from murine ova or 2-cell embryos. However, all the immunoinfertile Fab', but not fertile control Fab', specifically recognized a protein band in the M, 25 k 2 kD region, on the Western blots of extract from murine blastocyst stage embryos. In addition, Fab ' from one immunoinfertile serum, which inhibited embryonic development, reacted specifically with a protein band in the lower molecular range (approximate M, 12 kD) on Western blot involving exact from blastocysts. Fab' antibodies of sera from vasectomized men did not react with any specific protein band on blots of extracts from murine ova, 2-cell embryo, or blastocyst. These results suggest that ASAs from some immunoinfertile patients and vasectomized men, especially those reacting with 12-kD blastocyst protein, are capable of affecting preimplantation embryonic development in mice, and thus may contribute toward immunologically medicated infertility both at fertilization and postfertilization stages.

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Immunologic analysis of sperm-immobilizing factor found in sera of women with unexplained sterility

Cited by 388 publications

References 10 publications

A Comparison of Screening Tests for Anti-Sperm Activity in the Serum of Infertile Women

A Comparison of Screening Tests for Anti-Sperm Activity in the Serum of Infertile Women

Isoantigenicity of Rabbit Sperm, Testis, and Their Extracts as Demonstrated by Western Blot Enzyme Immunobinding Procedure

Effects of Human Antisperm Antibodies on Development of Preimplantation Embryos

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