Immunological Analysis of Abnormal Binding of Thyroid Hormone in the Gamma-Globulin

Radioimmunoassay for Estimation of Thyroglobulin in Human Serum

“…In the previous paper we proposed that in some patients with Hashimoto's thyroiditis denatured TG may be a trigger for autoimmune thyroiditis. (Ochi, et al, 1972). There is however no doubt that the existence of serum TG may have a relation to immunological tolerance of TG and also to the etiology of autoimmune thyroiditis.…”

“…In a previous paper, we suggested the release of denatured TG into the blood from the thyroid gland as an etiologic factor of chronic thyroiditis (Ochi et al, 1972). For this reason we set up a RIA for the determination of human TG using a specific antibody against TG and examined serum TG levels in normal subjects and in the patients with various thyroidal diseases.…”

Problems on the specificity of iodinated insulin binding by sera of patients with autoimmune thyroid disorders.

“…It has been reported that serum immunoglobulin of some patients with autoimmune thyroiditis or other thyroid diseases binds T3 and/or T4 (Robbins et al, 1956;Premachandra and Blumenthal, 1967;Ochi et al, 1972;Staeheli et al, 1975). This abnormal binding is, in general, more commonly observed in patients with higher titers of anti-thyroglobulin antibody, although Wu and Green (1976) reported a case of T3-specific binding without elevated titers of antibodies to thyroglobulin or thyroid microsomes.…”

“…This abnormal binding is, in general, more commonly observed in patients with higher titers of anti-thyroglobulin antibody, although Wu and Green (1976) reported a case of T3-specific binding without elevated titers of antibodies to thyroglobulin or thyroid microsomes. The binding of T3 and T4 by immunoglobulin can be produced also in experimental animals, by actively immunizing them with thyroglobulin or denatured thyroglobulin (Chopra et al, 1971;Ochi et al, 1972). The specificity of binding apparently differs with different sera, but the binding of thyronine derivatives is usually stronger than that of iodotyrosines.…”

“…Abnormal binding of thyroid hormones to gamma globulins in patients with diseases of thyroid has been reported (Robbins et al, 1956;Premachandra and Blumenthal, 1967;Ochi et al, 1972;Staeheli et al, 1975). Graves' disease and Hashimoto's thyroiditis are often associated with various forms of autoimmunity (De Groot and Stanbury, 1975;Doniach et al, 1963).…”