Immunological localization of Bacillus thuringiensis serovar israelensis toxins in midgut cells of intoxicated Anopheles gambiae larvae (Diptera: Culicidae)

Ravoahangimalala, Olga Ramilijaona; Charles, Jean‐François; Schoeller-Raccaud, J.

doi:10.1016/0923-2508(93)90011-p

Cited by 48 publications

(31 citation statements)

References 20 publications

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“…The main structural differences between the cardia, gastric caecae, the anterior stomach and the posterior stomach have been previously shown [11,21,22]: gastric caecae and the posterior stomach harbour very long and thin apical microvilli, whereas in the cardia and in the anterior stomach cells they are shorter.…”

Section: Resultsmentioning

confidence: 99%

“…Non-specific binding to mosquito midgut cells was reduced by adsorbing the IgG solution on freshly ground fourth instar larvae of A. gambiae, as previously described [11].…”

Section: Preparation Of Immunoglobulins Against Bti Icpsmentioning

confidence: 99%

“…Nevertheless, the contribution of CytA in the expression of the toxicity remains unclear. Indeed, it has been previously demonstrated that CrylVD and CytA were immunologically detected, both bound on apical microvilli of A. gambiae larval midgut cells, after in vivo intoxication with purified Bti crystals [11]. Other B. thuringiensis ICPs have also been shown to bind specifically, in vivo and in vitro, to midgut brush border membranes of Lepidoptera and Coleoptera larvae [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…Purified crystals from Bti strain 4Q2-72 were solubilized in alkali, injected into rabbits and the immunoglobulins (lgGs) purified from the collected antisera using ammonium sulphate precipitation and anionexchange chromatography as previously described [11]. Non-specific binding to mosquito midgut cells was reduced by adsorbing the IgG solution on freshly ground fourth instar larvae of A. gambiae, as previously described [11].…”

Section: Preparation Of Immunoglobulins Against Bti Icpsmentioning

confidence: 99%

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In vitro binding of Bacillus thuringiensis var. israelensis individual toxins to midgut cells of Anopheles gambiae larvae (Diptera: Culicidae)

Ravoahangimalala¹,

Charles²

1995

FEBS Letters

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Section: Resultsmentioning

confidence: 99%

“…Non-specific binding to mosquito midgut cells was reduced by adsorbing the IgG solution on freshly ground fourth instar larvae of A. gambiae, as previously described [11].…”

Section: Preparation Of Immunoglobulins Against Bti Icpsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Preparation Of Immunoglobulins Against Bti Icpsmentioning

confidence: 99%

See 2 more Smart Citations

In vitro binding of Bacillus thuringiensis var. israelensis individual toxins to midgut cells of Anopheles gambiae larvae (Diptera: Culicidae)

Ravoahangimalala¹,

Charles²

1995

FEBS Letters

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“…While cells of the posterior midgut eventually die, they never stain with AO. The bimodal staining pattern does not seem to be related to the relative binding of toxin to the gut epithelia since Ravoahangimalala and Charles (1995) reported that the toxin binds preferentially to the gastric caeca and posterior midgut, and less so to cells of the anterior midgut.…”

Section: Discussionmentioning

confidence: 99%

A Bacillus thuringiensis δ-endotoxin induces programmed cell death in mosquito larvae

Smouse

Nishiura

1997

Cell Death Differ

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We present evidence that a d-endotoxin isolated from Bacillus thuringiensis subsp.israelensis induces programmed cell death in polytene midgut cells of Culex pipiens larvae. After exposure to toxin, polytene nuclei in the anterior region of the larval midgut undergo many of the morphological and physiological changes which are characteristic of apoptosis, including the ability to stain with the vital dye, acridine orange, and fragmentation of nuclear DNA as demonstrated by agarose gel electrophoresis and in situ TUNEL labeling. The temporal sequence of toxin ingestion, acridine orange staining and larval death suggests a cause and effect relationship between programmed cell death and larval death. Amino sugars that interfere with toxicity also interfere with the time course of acridine orange staining of larval polytene nuclei. The toxin first causes programmed cell death of anterior midgut and gastric caeca cells and, subsequently, posterior midgut cells. This pattern is similar to the temporal sequence of larval polytene cell death that occurs during metamorphosis. From the size and distribution of the nuclei that are stained with acridine orange, it appears that only polytene midgut cells are affected by toxin and that the diploid regenerative cell are not affected.

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Fine Structural Changes in the Ileum of Mice Fed on δ-Endotoxin-Treated Potatoes and Transgenic Potatoes

Fares

El-Sayed

1998

Nat. Toxins

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The present work has been designed to study the effect of feeding on transgenic potatoes, which carry the CryI gene of Bacillus thuringiensis var. kurstaki strain HD1, on the light and electron microscopic structure of the mice ileum, in comparison with feeding on potatoes treated with the ‘δ‐endotoxin’ isolated from the same bacterial strain. The microscopic architecture of the enterocytes of the ileum of both groups of mice revealed certain common features such as the appearance of mitochondria with signs of degeneration and disrupted short microvilli at the luminal surface. However, in the group of mice fed on the ‘δ‐endotoxin’, several villi appeared with an abnormally large number of enterocytes (151.8 in control group versus 197 and 155.8 in endotoxin and transgenic‐treated groups, respectively). Fifty percent of these cells were hypertrophied and multinucleated. The mean area of enterocyte was significantly increased (105.3 µm2 in control group versus 165.4 µm2 and 116.5 µm2 in endotoxin and transgenic‐treated groups, respectively). Several forms of secondary lysosomes or auotophagic vacuoles were recognized in these cells. These changes were confirmed with the scanning electron microscope which revealed a remarkable increase in the topographic contour of enterocytes (23 µm in control group versus 44 µm and 28 µm in endotoxin and transgenic‐treated groups, respectively) at the divulged surface of the villi. The basal lamina along the base of the enterocytes was damaged at several foci. Several disrupted microvilli appeared in association with variable‐shaped cytoplasmic fragments. Some of these fragments contained endoplasmic reticulum, as well as ring‐shaped annulate lamellae. In addition, the Paneth cells were highly activated and contained a large number of secretory granules. These changes may suggest that δ‐endotoxin‐treated potatoes resulted in the development of hyperplastic cells in the mice ileum. Although mild changes are reported in the structural configuration of the ileum of mice fed on transgenic potatoes, nevertheless, thorough tests of these new types of genetically engineered crops must be made to avoid the risks before marketing. Copyright © 1998 John Wiley & Sons, Ltd.

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Immunological localization of Bacillus thuringiensis serovar israelensis toxins in midgut cells of intoxicated Anopheles gambiae larvae (Diptera: Culicidae)

Cited by 48 publications

References 20 publications

In vitro binding of Bacillus thuringiensis var. israelensis individual toxins to midgut cells of Anopheles gambiae larvae (Diptera: Culicidae)

In vitro binding of Bacillus thuringiensis var. israelensis individual toxins to midgut cells of Anopheles gambiae larvae (Diptera: Culicidae)

A Bacillus thuringiensis δ-endotoxin induces programmed cell death in mosquito larvae

Fine Structural Changes in the Ileum of Mice Fed on δ-Endotoxin-Treated Potatoes and Transgenic Potatoes

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