Immunomagnetic Diffractometry for Detection of Diagnostic Serum Markers

Acharya, Ghanashyam; Chang, Chun Li; Doorneweerd, Derek D.; Vlashi, Erina; Henne, Walter A.; Hartmann, Lynn C.; Low, Philip S.; Savran, Cagri A.

doi:10.1021/ja073094m

Cited by 58 publications

(48 citation statements)

References 38 publications

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“…It is known that immunomagnetic diffractometry for the detection of various important biomolecules related to diseases has shown potential as a simple and sensitive method [22,25]. In this paper, we demonstrated that the magnetic accumulation-based signal amplification method can be easily integrated with the existing immunomagnetic diffractometry methods.…”

Section: Discussionmentioning

confidence: 87%

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Detection of Proteins Using Nano Magnetic Particle Accumulation-Based Signal Amplification

İçöz

Mzava

2016

Applied Sciences

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Abstract:We report a biosensing method based on magnetic particles where coated magnetic particles are used for immunomagnetic separation, and uncoated magnetic particles are used for signal enhancement. To quantify the signal amplification, optical micrographs are analyzed to measure changes in pixel area and pixel intensity. Microcontact-printed surface receptors are arranged in alternating lines on gold chips, enabling differential calculations. In a model experiment, target molecules-streptavidin-are first captured and separated by biotin-coated magnetic particles, and then exposed to a gold surface functionalized with biotin-coupled bovine serum albumin, forming a sandwich assay. Applying a magnetic field and introducing uncoated magnetic particles resulted in accumulation around magnetic particles in the sandwich assay and enhancement of the contrast to noise ratio at least by eight-fold in a range of 0.1-100 µM.

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Section: Discussionmentioning

confidence: 87%

“…After reviewing the literature [22], the incubation times and concentrations related to PDMS stamping were optimized by trial and error. The PDMS stamp that contains 20 µm alternating lines was washed using an ultrasonic cleaner using 70% ethanol and rinsed using 70% ethanol followed by deionized (DI) water.…”

Section: Immobilization Of Bbsa On Gold Chipsmentioning

confidence: 99%

Detection of Proteins Using Nano Magnetic Particle Accumulation-Based Signal Amplification

İçöz

Mzava

2016

Applied Sciences

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“…Biosensors based on micro-contact printed probes and immunomagnetic beads were used to detect biologically significant target molecules such as cancer related folate receptor [9], and platelet-derived growth factor (PDGF) [8], and cardiovascular disease related s-adenosyl homocusteine [7]. In these biosensing applications the number of beads on the surface is proportional to the target concentration.…”

Section: Resultsmentioning

confidence: 99%

“…The printed lines are for sensing and the spacing between printed lines can be used for control, the alternating pattern enables diffraction measurements [8,9]. Different methods such as atomic force microscopy (AFM) [4], fluorescence microscopy [3], and X-ray photoelectron spectroscopy (XPS) [10] are used to quantify the binding of biomolecules on micro-contact printed lines.…”

Section: Introductionmentioning

confidence: 99%

“…Micro/nano immunomagnetic beads enable target capture and separation in biosensing applications and due to these important features they are also employed on micro-contact printed lines to transfer target molecules to the sensor surface, and to form sandwich assays and reflective surfaces for laser diffraction measurements [7,9]. 2 of 9 Cell phone microscopy is gaining in popularity due to being low cost, and the wide-spread use of cell phones [11].…”

Section: Introductionmentioning

confidence: 99%

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Image Processing and Cell Phone Microscopy to Analyze the Immunomagnetic Beads on Micro-Contact Printed Gratings

İçöz

2016

Applied Sciences

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Abstract:In this paper we report an ultra-low-cost spherical ball lens based cell phone microscopy and image processing algorithms to analyze the amount of immunomagnetic beads on micro-contact printed gratings. The spherical ball lens provides approximately 100× magnification but the recorded images are not clear and are noisy. By using the image-processing algorithms, the noise can be reduced and the images can be enhanced to quantify the amount of immunomagnetic beads on micro-contact printed lines. This method, which is portable and low-cost, can be an alternative read out mechanism for biosensing applications using immunomagnetic beads on micro-contact printed surface receptors. Further, 0.0335 mg/mL was the lowest magnetic bead concentration that could be detected above the inherent noise level of the spherical ball lens.

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3D‐Bioprinted Inflammation Modulating Polymer Scaffolds for Soft Tissue Repair

et al. 2020

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creates a defect that intra-abdominal contents may protrude through. [1] The most common types of hernias are incisional, inguinal, femoral, umbilical and hiatal hernias. [2] Hernia repair for many defects is performed by the surgical implantation of a prosthetic mesh to firmly support and reinforce the damaged abdominal wall and facilitate the healing process (Figure 1A,B). Each year, over 400 000 incisional hernia repair surgeries are performed with a cost of ≈$15 billion in US healthcare expenditures. [3-5] Prosthetic hernia mesh implants are developed using synthetic, biologic, and coated materials. [6,7] Despite their specific advantages, these mesh implants are not very effective in minimizing potential adverse postsurgical complications. [8] Surgical hernia repair with mesh implants mostly fail due to the formation of visceral adhesions, hardening, and shrinking of the mesh after its implantation. Visceral adhesions are fibrous tissues developed from the underlying serosal membrane of stomach, intestine or colon, that attach to the implanted mesh. [9] These adhesions are mainly composed of collagen and fibroblasts that grow on the mesh and adhere to the nearby tissue, nerves and organs. [10] The mesh shrinks as the adhesions grow and scar tissue hardens, thus forming a hard, fibrous mass that may cause chronic pain, bowel obstruction, enteric fistula, infertility, poor quality of life, and failure of the surgical hernia repair. [11-13] To remove the failed hernia mesh, a complicated surgery needs to be performed, wherein the mesh must be peeled off bladder, stomach, intestine, colon, or a major blood vessel, that may adversely affect the clinical outcomes. [14] To minimize adhesions formation, graft contraction, and foreign body reactions, absorbable and biological meshes have been developed. However, these meshes are not significantly effective because of very high hernia recurrence rates. [15-17] Causative factors for adverse complications arising due to surgical mesh implantation are chronic inflammatory responses, poor mesh-tissue integration, rapid degradation of the materials, surface chemistry and topochemical design of the mesh. [18,19] We herein present the development of an intrinsically inflammation modulating 3D-fabricated biomaterial scaffold (bioscaffold) for soft tissue repair and demonstrate its in vivo efficacy in a rat ventral hernia Development of inflammation modulating polymer scaffolds for soft tissue repair with minimal postsurgical complications is a compelling clinical need. However, the current standard of care soft tissue repair meshes for hernia repair is highly inflammatory and initiates a dysregulated inflammatory process causing visceral adhesions and postsurgical complications. Herein, the development of an inflammation modulating biomaterial scaffold (bioscaffold) for soft tissue repair is presented. The bioscaffold design is based on the idea that, if the excess proinflammatory cytokines are sequestered from the site of injury by the surgical implantation of a bioscaffold,...

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Immunomagnetic Diffractometry for Detection of Diagnostic Serum Markers

Cited by 58 publications

References 38 publications

Detection of Proteins Using Nano Magnetic Particle Accumulation-Based Signal Amplification

Detection of Proteins Using Nano Magnetic Particle Accumulation-Based Signal Amplification

Image Processing and Cell Phone Microscopy to Analyze the Immunomagnetic Beads on Micro-Contact Printed Gratings

3D‐Bioprinted Inflammation Modulating Polymer Scaffolds for Soft Tissue Repair

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