Immunomagnetic separation as an alternative to enrichment broths for Salmonella detection

Mansfield, Lucielle P.; Forsythe, Stephen

doi:10.1111/j.1472-765x.1993.tb01375.x

Cited by 54 publications

(24 citation statements)

References 9 publications

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“…The good performance of the IMS-plating method is consistent with previous results, which found IMS associated to plating on selective agar better than conventional culture method for salmonellae detection (3,8,9,15). In contrast, other studies have reported a poor performance of the method that combines IMS with plating on selective agar for Salmonella detection in chicken samples (13,14).…”

Section: Resultssupporting

confidence: 89%

Detection of salmonella sp in chicken cuts using immunomagnetic separation

Conceição¹,

Moreira²,

Ramos³

et al. 2008

Braz. J. Microbiol.

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The immunomagnetic separation (IMS) is a technique that has been used to increase sensitivity and specificity and to decrease the time required for detection of Salmonella in foods through different methodologies. In this work we report on the development of a method for detection of Salmonella in chicken cuts using in house antibody-sensitized microspheres associated to conventional plating in selective agar (IMS-plating). First, protein A-coated microspheres were sensitized with polyclonal antibodies against lipopolysacharide and flagella from salmonellae and used to standardize a procedure for capturing Salmonella Enteritidis from pure cultures and detection in selective agar. Subsequently, samples of chicken meat experimentally contaminated with S. Enteritidis were analyzed immediately after contamination and after 24h of refrigeration using three enrichment protocols. The detection limit of the IMS-plating procedure after standardization with pure culture was about 2x10 CFU/mL. The protocol using non-selective enrichment for 6-8h, selective enrichment for 16-18h and a post-enrichment for 4h gave the best results of S. Enteritidis detection by IMS-plating in experimentally contaminated meat. IMSplating using this protocol was compared to the standard culture method for salmonellae detection in naturally contaminated chicken cuts and yielded 100% sensitivity and 94% specificity. The method developed using in house prepared magnetic microespheres for IMS and plating in selective agar was able to diminish by at least one day the time required for detection of Salmonella in chicken products by the conventional culture method.

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Section: Resultssupporting

confidence: 89%

Detection of salmonella sp in chicken cuts using immunomagnetic separation

Conceição¹,

Moreira²,

Ramos³

et al. 2008

Braz. J. Microbiol.

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show abstract

“…Paramagnetic beads are coated with polyclonal antibodies, which can target and separate Salmonella cells in a mixed suspension with no loss of viability, producing a normal isolate for further confirmation. IMS can eliminate the need for selective enrichment and reduce the time required for conventional methods by as much as 24 h (15). IMS has also been used in conjunction with other rapid detection methods, including enzyme-linked immunosorbent assays (ELISAs), conductance microbiology, electrochemiluminescence, and PCR (6,8,12,20,34).…”

mentioning

confidence: 99%

Development and Optimization of a Novel Immunomagnetic Separation- Bacteriophage Assay for Detection of Salmonella enterica Serovar Enteritidis in Broth

Favrin¹,

Jassim²,

Griffiths

2001

Appl Environ Microbiol

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Salmonella is the second-leading cause of food-borne illness in most developed countries, causing diarrhea, cramps, vomiting, and often fever. Many rapid methods are available for detection of Salmonella in foods, but these methods are often insensitive or expensive or require a high degree of technical ability to perform. In this paper we describe development and characterization of a novel assay that utilizes the normal infection cycle of bacteriophage SJ2 for detection of Salmonella enterica serovar Enteritidis in broth. The assay consists of four main stages: (i) capture and concentration of target cells by using immunomagnetic separation (IMS); (ii) infection of the target bacterium with phage; (iii) amplification and recovery of progeny phage; and (iv) assay of progeny phage on the basis of their effect on a healthy population of host cells (signal-amplifying cells). The end point of the assay can be determined by using either fluorescence or optical density measurements. The detection limit of the assay in broth is less than 10 4 CFU/ml, and the assay can be performed in 4 to 5 h. The results of this study demonstrate that the IMS-bacteriophage assay is a rapid, simple, and sensitive technique for detection of Salmonella serovar Enteritidis in broth cultures which can be applied to preenriched food samples.

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“…Detailed literature surveys showed that the presence of Salmonella bacteria examined by IMS method is generally found in chicken [18] and pork, cacao, powdered milk and sausage [8] ; however, minced meat samples are found to be investigated in a very low number of publications. In the current study, as an alternative to the conventional culture method, IMS method showed that only three samples out of fifty samples examined had Salmonella bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…Immunomagnetic separation (IMS) method is generally used in great efficiency in acquisition of damaged cells after enrichment process or in isolation of specific pathogen bacteria from heterogeneous cell suspensions [7][8][9][10][11] . Although IMS method takes the place of enrichment media, when it is used in combination with pre-enrichment and enrichment media, positive results are increased [12][13][14] .…”

Section: Anahtar Sözcükler: Geleneksel Kültür Metodu (Iso 6579 Referamentioning

confidence: 99%

Comparison of Different Methods for the Detection of Salmonella spp. in Minced Meat Samples

Ozer¹,

Kimiran-Erdem²

2013

Kafkas Univ Vet Fak Derg

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The presence of Salmonella spp. in minced meat that is consumed in nine different sites of Istanbul is evaluated by using conventional culture (ISO 6579:2002), immunomagnetic separation (IMS) and fluorescent in situ hybridization (FISH) methods. Salmonella spp. was isolated from five of 50 (20%) minced meat with ISO 6579 method, and three of 50 (6%) minced meat using IMS method. Bacteria isolated from both ISO and IMS methods were identified as Salmonella choleraesuis ssp. arizonae and Salmonella spp. The presence of Salmonella spp. was determined from 37 of 50 (74%) minced meat by using FISH method. In the current study, it has been shown that ISO 6579 method was found more to be susceptible than IMS method for determining presence of Salmonella spp., FISH method is the best method to determine the presence of Salmonella spp. Even if the quick determination of the epidemics of international importance occured as a result of the contamination by pathogens derivated from foods, the results of the use of new methods should be supported by the conventional culture method. Keywords: Conventional culture method (ISO 6579 reference method), Fluorescent in situ hybridization (FISH) method,Immunomagnetic separation method, Minced meat, Salmonella Kıyma Örneklerinde Salmonella spp. Tespitinde Farklı Yöntemlerin Karşılaştırılması Özetİstanbul'un dokuz farklı semtinde tüketime sunulan kıyma örneklerinde geleneksel kültür yöntemi (ISO), immünomanyetik ayırma (İMA) ve floresanlı yerinde hibritleme (FISH) yöntemleri kullanılarak Salmonella cinsi bakterilerin varlığı tespit edilmiştir. İncelenen 50 kıyma örneğinin, geleneksel kültür yöntemi ile 5' inden (20%), İMA yöntemi ile 3' ünden (6%) Salmonella cinsi bakteri izole edilmiştir. Gerek geleneksel kültür yöntemi gerekse de İMA yönteminde besiyeri üzerinde üreyen kolonilerden elde edilen izolatlar Salmonella choleraesuis ssp. arizonae ve Salmonella spp. olarak tanımlanmıştır. İncelenen 50 kıyma örneğinin 37'sinde (74%) ise FISH yöntemi ile Salmonella cinsi bakterilerin varlığı tespit edilmiştir. Çalışmamızda Salmonella cinsi bakterilerin varlığını belirlemede geleneksel kültür yönteminin, İMA yöntemine göre daha duyarlı bir yöntem olduğu, FISH yönteminin ise Salmonella cinsi bakterilerin varlığını belirlemede en iyi yöntem olduğu tespit edilmiştir. Gıda kaynaklı patojenlerle kontaminasyon sonucunda ortaya çıkan uluslararası önemdeki salgınların hızlı tespiti ne kadar önemli olsa da, uygulanan yeni yöntemlerin sonuçlarının geleneksel kültür yöntemi ile desteklenmesi gerekmektedir.

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Immunomagnetic separation as an alternative to enrichment broths for Salmonella detection

Cited by 54 publications

References 9 publications

Detection of salmonella sp in chicken cuts using immunomagnetic separation

Detection of salmonella sp in chicken cuts using immunomagnetic separation

Development and Optimization of a Novel Immunomagnetic Separation- Bacteriophage Assay for Detection of Salmonella enterica Serovar Enteritidis in Broth

Comparison of Different Methods for the Detection of Salmonella spp. in Minced Meat Samples

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