2021
DOI: 10.3390/s21185992
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Immunomagnetic Separation Improves the Detection of Mycobacteria by Paper-Based Lateral and Vertical Flow Immunochromatographic Assays

Abstract: This work addresses a method that combines immunomagnetic separation (IMS) and paper-based nucleic acid immunochromatographic assay for the sensitive detection of Mycolicibacterium fortuitum (basonym Mycobacterium fortuitum) In particular, the preconcentration of the bacteria was achieved by using magnetic particles modified with an antibody specific towards mycobacteria. Following the IMS, the bacteria were lysed, and the genome was amplified by double-tagging PCR, using a set of primers specific for the 16S … Show more

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Cited by 10 publications
(5 citation statements)
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“…Enhancing the sensitivity of a LFIA is critical for the detection of many biomarkers. In recent years, great efforts have been devoted to developing new strips with high sensitivity, mainly by conjugating antigens or antibodies to nanomaterials, such as gold nanoparticles (AuNPs), 3 magnetic nanoparticles, 6,7 quantum dots, 8 and fluorescent microspheres. 9,10 Among these methods, conjugating antibodies and horseradish peroxidase (HRP) to the AuNPs simultaneously can lead to effective and ultrasensitive chemiluminescent (CL) immunoassays (CL-LFIAs).…”
Section: ■ Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…Enhancing the sensitivity of a LFIA is critical for the detection of many biomarkers. In recent years, great efforts have been devoted to developing new strips with high sensitivity, mainly by conjugating antigens or antibodies to nanomaterials, such as gold nanoparticles (AuNPs), 3 magnetic nanoparticles, 6,7 quantum dots, 8 and fluorescent microspheres. 9,10 Among these methods, conjugating antibodies and horseradish peroxidase (HRP) to the AuNPs simultaneously can lead to effective and ultrasensitive chemiluminescent (CL) immunoassays (CL-LFIAs).…”
Section: ■ Introductionmentioning
confidence: 99%
“…Enhancing the sensitivity of a LFIA is critical for the detection of many biomarkers. In recent years, great efforts have been devoted to developing new strips with high sensitivity, mainly by conjugating antigens or antibodies to nanomaterials, such as gold nanoparticles (AuNPs), magnetic nanoparticles, , quantum dots, and fluorescent microspheres. , Among these methods, conjugating antibodies and horseradish peroxidase (HRP) to the AuNPs simultaneously can lead to effective and ultrasensitive chemiluminescent (CL) immunoassays (CL-LFIAs). , Compared with other optical methods, the CL-LFIAs are highly specific and ultrasensitive with high signal-to-noise ratios . However, these assays usually suffer from cross-reactivity issues that would cause false-positive errors to further lower the detection accuracy.…”
Section: Introductionmentioning
confidence: 99%
“…In consequence, a series of strategies have been integrated with VFA for sensitivity improvement. [20][21][22] Among these technologies, surface enhanced Raman scattering (SERS) has impressive advantages for VFA, including distinctive fingerprint signals, excellent multiplexing, and ultrasensitivity. 23 Recently, SERS nanotags have been introduced into immunoassay for improving the LOD, enriching the quantitative ability and multiplex detection.…”
Section: Introductionmentioning
confidence: 99%
“…Techniques have been developed to improve the sensitivities of LFAs, including kinetics and transport control ( Yang et al, 2013 ; Rivas et al, 2014 ; Tang et al, 2017 ; Ishii et al, 2018 ), biochemical signal amplification ( Hu et al, 2013 ; Parolo et al, 2013 ; Panferov et al, 2016 ), improved labeling ( Choi et al, 2010 ), and sample enrichment. Sample enrichment techniques include centrifugal filtration ( Corstjens et al, 2015 ), immunomagnetic separation ( Panferov et al, 2017 ; Ben Aissa et al, 2021 ), electrophoretic and phasic separation ( Wu et al, 2014 ; Chiu et al, 2015 ; Kim et al, 2017 ), isotachophoresis ( Moghadam et al, 2015 ), dialysis ( Tang et al, 2016 ), and test-zone pre-enrichment ( Zhang et al, 2020 ). These systems are not suitable for low-resource, point-of-care settings as they require expensive reagents, equipment, and/or complex procedures.…”
Section: Introductionmentioning
confidence: 99%