Immunomodulation by mesenchymal stem cells is sex dependent

Mckinnirey, Flyn

doi:10.1016/j.jcyt.2019.03.502

Cited by 3 publications

(2 citation statements)

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“…One of the aims of our study was to examine potential sex differences in the viability, proliferation, and differentiation potential in cells from dogs and cats. Sex-related differences have been previously reported in regard to the neurogenic potential ( 54 , 55 ), immunomodulation ( 56 ), and therapeutic efficacy ( 57 ) of stem cells in humans and animal models. In our study, the only differences between sexes were observed in osteogenic potential, which was different with cells from dogs and cats at passage 2.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Canine and Feline Adipose-Derived Mesenchymal Stem Cells/Medicinal Signaling Cells With Regard to Cell Surface Marker Expression, Viability, Proliferation, and Differentiation Potential

2021

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Remarkable immunomodulatory abilities of mesenchymal stem cells, also called multipotent mesenchymal stromal cells or medicinal signaling cells (MSCs), have entailed significant advances in veterinary regenerative medicine in recent years. Despite positive outcomes from MSC therapies in various diseases in dogs and cats, differences in MSC characteristics between small animal veterinary patients are not well-known. We performed a comparative study of cells' surface marker expression, viability, proliferation, and differentiation capacity of adipose-derived MSCs (ADMSCs) from dogs and domestic cats. The same growth media and methods were used to isolate, characterize, and culture canine and feline ADMSCs. Adipose tissue was collected from 11 dogs and 8 cats of both sexes. The expression of surface markers CD44, CD90, and CD34 was detected by flow cytometry. Viability at passage 3 was measured with the hemocytometer and compared to the viability measured by flow cytometry after 1 day of handling. The proliferation potential of MSCs was measured by calculating cell doubling and cell doubling time from second to eighth passage. Differentiation potential was determined at early and late passages by inducing cells toward adipogenic, osteogenic, and chondrogenic differentiation using commercial media. Our study shows that the percentage of CD44+CD90+ and CD34−/− cells is higher in cells from dogs than in cells from cats. The viability of cells measured by two different methods at passage 3 differed between the species, and finally, canine ADMSCs possess greater proliferation and differentiation potential in comparison to the feline ADMSCs.

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Section: Discussionmentioning

confidence: 99%

Comparison of Canine and Feline Adipose-Derived Mesenchymal Stem Cells/Medicinal Signaling Cells With Regard to Cell Surface Marker Expression, Viability, Proliferation, and Differentiation Potential

2021

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“…Furthermore, male cells secreted significantly higher amounts of immunomodulatory cytokines (4 out of 6 analyzed cytokines: GAL9, IL10, IL1B, and MMP3) compared to female cells ( Figure 7B ) consistent with our gene expression data. This suggests there are significant sex-based differences in immunomodulatory potential of these hMSCs, and this has been observed in vitro [70, 71] by other groups. As seen in Table 1 various donors had co-morbidities while for others such health information was not provided.…”

Section: Discussionmentioning

confidence: 74%

Donor Variability in Human Mesenchymal Stem Cell Osteogenic Response as a Function of Passage Conditions and Donor Sex

Kolliopoulos,

Tiffany,

Polanek

et al. 2023

Preprint

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Contemporary tissue engineering efforts often seek to use mesenchymal stem cells (MSCs) due to their potential to differentiate to various tissue-specific cells and generate a pro-regenerative secretome. While MSC differentiation and therapeutic potential can differ as a function of matrix environment, it may also be widely influenced as a function of donor-to-donor variability. Further, effects of passage number and donor sex may further convolute the identification of clinically effective MSC-mediated regeneration technologies. We report efforts to adapt a well-defined mineralized collagen scaffold platform to study the influence of MSC proliferation and osteogenic potential as a function of passage number and donor sex. Mineralized collagen scaffolds broadly support MSC osteogenic differentiation and regenerative potency in the absence of traditional osteogenic supplements for a wide range of MSCs (rabbit, rat, porcine, human). We obtained a library of bone marrow and adipose tissue derived stem cells to examine donor-variability of regenerative potency in mineralized collagen scaffolds. MSCs displayed reduced proliferative capacity as a function of passage duration. Further, MSCs showed significant sex-based differences. Notably, MSCs from male donors displayed significantly higher metabolic activity and proliferation while MSCs from female donor displayed significantly higher osteogenic response via increased alkaline phosphate activity, osteoprotegerin release, and mineral formation in vitro. Our study highlights the essentiality of considering MSC donor sex and culture expansion in future studies of biomaterial regenerative potential.

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Donor Sex and Passage Conditions Influence MSC Osteogenic Response in Mineralized Collagen Scaffolds

Kolliopoulos,

Tiffany,

Polanek

et al. 2024

Adv Healthcare Materials

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Contemporary tissue engineering efforts often seek to use mesenchymal stem cells (MSCs) due to their multi‐potent potential and ability to generate a pro‐regenerative secretome. While many have reported the influence of matrix environment on MSC osteogenic response, few have investigated the effects of donor and sex. Here, a well‐defined mineralized collagen scaffold is used to study the influence of passage number and donor‐reported sex on MSC proliferation and osteogenic potential. A library of bone marrow and adipose tissue‐derived stem cells from eight donors to examine donor viability in osteogenic capacity in mineralized collagen scaffolds is obtained. MSCs displayed reduced proliferative capacity as a function of passage duration. Further, MSCs showed significant sex‐associated variability in osteogenic capacity. Notably, MSCs from male donors displayed significantly higher cell proliferation while MSCs from female donors displayed significantly higher osteogenic response via increased alkaline phosphate activity, osteoprotegerin release, and mineral formation in vitro. The study highlights the essentiality of including donor‐reported sex as an experimental variable and reporting culture expansion in future studies of biomaterial regenerative potential.

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Immunomodulation by mesenchymal stem cells is sex dependent

Cited by 3 publications

References 0 publications

Comparison of Canine and Feline Adipose-Derived Mesenchymal Stem Cells/Medicinal Signaling Cells With Regard to Cell Surface Marker Expression, Viability, Proliferation, and Differentiation Potential

Comparison of Canine and Feline Adipose-Derived Mesenchymal Stem Cells/Medicinal Signaling Cells With Regard to Cell Surface Marker Expression, Viability, Proliferation, and Differentiation Potential

Donor Variability in Human Mesenchymal Stem Cell Osteogenic Response as a Function of Passage Conditions and Donor Sex

Donor Sex and Passage Conditions Influence MSC Osteogenic Response in Mineralized Collagen Scaffolds

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