Immunomodulatory activity-guided isolation and characterization of a novel polysaccharide from Atractylodis macrocephalae Koidz

Xue, Wenyao; Gao, Yue; Li, Qianwen; Lu, Qibin; Bian, Zhengying; Tang, Lei; Zeng, Yu; Chen, Cong; Guo, Wei

doi:10.1016/j.ijbiomac.2020.06.003

Cited by 35 publications

(13 citation statements)

References 38 publications

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“…HPGPC and HPLC are the main methods for determining the average molecular weight of RAMP [ 35 , 36 ]. Xue et al [ 37 ] extracted two polysaccharides RAMP1 and RAMP2 from Atractylodes Rhizoma and measured their molecular weights to be 1.851 × 10 6 Da and 4.354 × 103 Da, respectively. Another study [ 38 ] used a GPC-RI-MALS instrument (gel chromatography-differential-multiangle laser light scattering) to determine the average molecular weight of RAMP to be 4.748 × 103 Da.…”

Section: Physiochemical and Structural Features Of Rampmentioning

confidence: 99%

“…Lin et al [ 43 ] extracted an inulin-type polysaccharide from Atractylodes Rhizoma and then used electrospray ionization mass spectrometry (ESI-MS), NMR, and hydrophilic interaction liquid chromatography (HILIC) method to study its structure and found that the main chain of RAMP is composed of a-D-Glcp-[-(1 ⟶ 2)- β -D-Fruf-] 8 -(1 ⟶ 2)- β -D-Fruf. The NMR results suggested that RAMP2 contained a backbone chain mainly consisting of ⟶ 3- β -D-Glcp-(1 ⟶, ⟶ 3, 6- β -D-Glcp-(1 ⟶, ⟶ 6- β -D-Glcp-(1 ⟶, T- β -D-Glcp, ⟶ 4- α - GalpA-(1 ⟶, ⟶ 4- α -GalpA-OMe-(1 ⟶, ⟶ 5- α -Araf-(1 ⟶, ⟶ 4, 6- β -manp-(1 ⟶ and ⟶ 4- β -Galp-(1 ⟶ [ 37 ].…”

Section: Physiochemical and Structural Features Of Rampmentioning

confidence: 99%

“… AFM, TEM, and SEM images of Rhizoma Atractylodis Macrocephalae polysaccharides [ 32 , 37 , 39 ]. (a) AFM image of of PAM; (b) TEM spectrum of RAMP2 in purified water; (c) the morphology of RAMP2 at 10000×, scale bar = 5 μ m; (d) SEM image of RAMPtp at 30000×, scale bar = 1 μ m. …”

Section: Figurementioning

confidence: 99%

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Polysaccharides from Rhizoma Atractylodis Macrocephalae: A Review on Their Extraction, Purification, Structure, and Bioactivities

Luo

Cai

Zhang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Rhizoma Atractylodes macrocephala polysaccharide (RAMP), the main bioactive compound extracted from Rhizoma Atractylodes macrocephala (RAM), exhibits various biological activities in in vivo and in vitro methods, such as anti-inflammatory, antioxidant, antitumor, immunomodulatory, hepatoprotective effects, and other functions. This review systematically summarizes the recent research progress on the extraction, purification, structural characteristics, and biological activities of RAMP. We hope to provide a theoretical basis for further research on the application of RAMP in the fields of biomedicine and food.

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Section: Physiochemical and Structural Features Of Rampmentioning

confidence: 99%

Section: Physiochemical and Structural Features Of Rampmentioning

confidence: 99%

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Polysaccharides from Rhizoma Atractylodis Macrocephalae: A Review on Their Extraction, Purification, Structure, and Bioactivities

Luo

Cai

Zhang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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show abstract

“… 19 Atractylodis macrocephalae (AM) is a perennial herb of the composite family and its rhizome can be used as medicine. 20 AM shows various pharmacological activities, including anti-inflammatory, anti-oxidant, anti-aging, anti-tumor, anti-osteoporosis, anti-bacterial, nerve protection and liver protection, et al 21 However, polysaccharides from traditional Chinese medicine cannot achieve specific antiviral activity.…”

Section: Introductionmentioning

confidence: 99%

Assembly encapsulation of BSA and CCCH-ZAP in the sodium alginate/atractylodis macrocephalae system

Zhang

Fan

et al. 2022

RSC Adv.

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“…The Huangqi-Fangfeng drug pair prevents allergic airway remodeling by inhibiting the epithelial-mesenchymal transformation process via regulating the epithelial-derived TGF- β 1 [ 15 ]. The polysaccharide isolated from Baizhu enhances immunity by promoting the proliferation of CD4, CD8, and Treg cells [ 16 , 17 ]. However, the TCM compound has the characteristics of multiple components, multiple targets, and multiple pathways.…”

Section: Introductionmentioning

confidence: 99%

Network Pharmacology and Molecular Docking Study of Yupingfeng Powder in the Treatment of Allergic Diseases

Tao

2022

Evidence-Based Complementary and Alternative Medicine

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Objective. To explore the potential mechanisms of Yupingfeng Powder (YPFP) in the treatment of allergic diseases by using network pharmacology and molecular docking technology. Methods. The active components and targets of YPFP were screened by the TCMSP database. The targets associated with atopic dermatitis, asthma, allergic rhinitis, and food allergy were obtained from GeneCards and OMIM databases, respectively. The intersection of the above disease-related targets was identified as allergy-related targets. Then, allergy-related targets and YPFP-related targets were crossed to obtain the potential targets of YPFP for allergy treatment. A protein-protein-interaction (PPI) network and a drug-target-disease topology network were constructed to screen hub targets and key ingredients. Next, GO and KEGG pathway enrichment analyses were performed separately on the potential targets and hub targets to identify the biological processes and signaling pathways involved. Finally, molecular docking was conducted to verify the binding affinity between key ingredients and hub targets. Results. In this study, 45 active ingredients were identified from YPFP, and 48 allergy-related targets were predicted by network pharmacology. IL6, TNF, IL1B, PTGS2, CXCL8, JUN, CCL2, IL10, IFNG, and IL4 were screened as hub targets by the PPI network. However, quercetin, kaempferol, wogonin, formononetin, and 7-O-methylisomucronulatol were identified as key ingredients by the drug-target-disease topological network. GO and KEGG pathway enrichment analysis indicated that the therapeutic effect of YPFP on allergy involved multiple biological processes and signaling pathways, including positive regulation of fever generation, positive regulation of neuroinflammatory response, vascular endothelial growth factor production, negative regulation of cytokine production involved in immune response, positive regulation of mononuclear cell migration, type 2 immune response, and negative regulation of lipid storage. Molecular docking verified that all the key ingredients had good binding affinity with hub targets. Conclusion. This study revealed the key ingredients, hub targets, and potential mechanisms of YPFP antiallergy, and these data can provide some theoretical basis for subsequent allergy treatment and drug development.

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Immunomodulatory activity-guided isolation and characterization of a novel polysaccharide from Atractylodis macrocephalae Koidz

Cited by 35 publications

References 38 publications

Polysaccharides from Rhizoma Atractylodis Macrocephalae: A Review on Their Extraction, Purification, Structure, and Bioactivities

Polysaccharides from Rhizoma Atractylodis Macrocephalae: A Review on Their Extraction, Purification, Structure, and Bioactivities

Assembly encapsulation of BSA and CCCH-ZAP in the sodium alginate/atractylodis macrocephalae system

Network Pharmacology and Molecular Docking Study of Yupingfeng Powder in the Treatment of Allergic Diseases

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