Immunomodulatory Function of Egg White Peptides in RAW264.7 Macrophage Cells and Immunosuppressive Mice Induced by Cyclophosphamide

Zhang, Fan; Li, Junhua; Chang, Cuihua; Gu, Luping; Su, Yujie; Yang, Yanjun

doi:10.1007/s10989-022-10481-3

Cited by 3 publications

(3 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The animal experiment design has been slightly modified according to the existing research. 24,25,39 All experimental operations passed the ethical review o f a n i m a l e x p e r i m e n t s i n J i a n g s u P r o v i n c e ( N o . JN.No20230515b0810713 [195]).…”

Section: Analysis Of Content and Form Of Selenium In Egg Yolkmentioning

confidence: 99%

See 1 more Smart Citation

Egg Yolk Selenopeptides: Preparation, Characterization, and Immunomodulatory Activity

Zhao,

McClements,

et al. 2024

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

In this study, egg yolk selenium peptides (Se-EYP) were prepared using double-enzyme hydrolysis combined with a shearing pretreatment. The properties of the selenopeptides formed were then characterized, including their yield, composition, molecular weight distribution, antioxidant activity, in vitro digestion, and immunomodulatory activity. The peptide yield obtained after enzymatic hydrolysis using a combination of alkaline protease and neutral protease was 74.5%, of which 82.6% had a molecular weight <1000 Da. The selenium content of the lyophilized solid product was 4.01 μg/g. Chromatography−mass spectrometry analysis showed that 88.6% of selenium in Se-EYP was in the organic form, of which SeMet accounted for 60.3%, SeCys2 for 21.8%, and MeSeCys for 17.9%. After being exposed to in vitro simulated digestion, Se-EYP still had 65.1% of oligopeptides present, and the in vitro antioxidant activity was enhanced. Moreover, Se-EYP exhibited superior immune detection indices, including immune organ index, level of immune factors in the serum, histopathological changes in the spleen, and selenium content in the liver. Our results suggest that Se-EYP may be used as selenium-enriched ingredients in functional food products.

show abstract

Section: Analysis Of Content and Form Of Selenium In Egg Yolkmentioning

confidence: 99%

“…Two to three drops of mouse blood were collected in an anticoagulation tube, and the content of each immune cell in mouse blood was quantified by an automatic biochemical analyzer (BS-420, China). 39 2.9.3. Determination of Immunizing Factors in Mouse Serum.…”

Section: Analysis Of Content and Form Of Selenium In Egg Yolkmentioning

confidence: 99%

Egg Yolk Selenopeptides: Preparation, Characterization, and Immunomodulatory Activity

Zhao,

McClements,

et al. 2024

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The immunoregulatory impact of egg white peptides (EWP) on RAW264.7 macrophage cells and an immunosuppressive BALB/c mice model was evaluated by Zhang, F. et al The findings obtained from the in vitro experiments indicated that the administration of 100 mg/mL EWP resulted in a significant increase in macrophage activation and the secretion of NO, IL-6, IL-10, and TNF-a in RAW264.7 cells. Additionally, an increase in the production of cytokines such as IL-2, IL-6, IL-10, and TNF-a by splenocytes and peripheral blood leukocytes was observed in mice administered with a dose of 150 mg/kg/day of EWP (148). Another study was conducted to investigate the immunomodulatory impact of peptides derived from Selenium-enriched EWP (Se-EWP) on mice that had been immunosuppressed due to cyclophosphamide administration.…”

Section: Alkalineneutral Proteasementioning

confidence: 99%

Bioactive peptides: an alternative therapeutic approach for cancer management

Ghadiri,

Javidan,

Sheikhi

et al. 2024

Front. Immunol.

View full text Add to dashboard Cite

Cancer is still considered a lethal disease worldwide and the patients’ quality of life is affected by major side effects of the treatments including post-surgery complications, chemo-, and radiation therapy. Recently, new therapeutic approaches were considered globally for increasing conventional cancer therapy efficacy and decreasing the adverse effects. Bioactive peptides obtained from plant and animal sources have drawn increased attention because of their potential as complementary therapy. This review presents a contemporary examination of bioactive peptides derived from natural origins with demonstrated anticancer, ant invasion, and immunomodulation properties. For example, peptides derived from common beans, chickpeas, wheat germ, and mung beans exhibited antiproliferative and toxic effects on cancer cells, favoring cell cycle arrest and apoptosis. On the other hand, peptides from marine sources showed the potential for inhibiting tumor growth and metastasis. In this review we will discuss these data highlighting the potential befits of these approaches and the need of further investigations to fully characterize their potential in clinics.

show abstract

Gastrointestinal digestion of food proteins: Anticancer, antihypertensive, anti-obesity, and immunomodulatory mechanisms of the derived peptides

Yan,

Gui,

Liu

et al. 2024

Food Research International

View full text Add to dashboard Cite

Immunomodulatory Function of Egg White Peptides in RAW264.7 Macrophage Cells and Immunosuppressive Mice Induced by Cyclophosphamide

Cited by 3 publications

References 41 publications

Egg Yolk Selenopeptides: Preparation, Characterization, and Immunomodulatory Activity

Egg Yolk Selenopeptides: Preparation, Characterization, and Immunomodulatory Activity

Bioactive peptides: an alternative therapeutic approach for cancer management

Gastrointestinal digestion of food proteins: Anticancer, antihypertensive, anti-obesity, and immunomodulatory mechanisms of the derived peptides

Contact Info

Product

Resources

About