Immunomodulatory, hemolytic and cytotoxic activity potentials of triterpenoid saponins from eight Cephalaria species

Sarikahya, Nazli Boke; Nalbantsoy, Ayşe; Top, Hilal; Göktürk, Ramazan Süleyman; Sümbül, Hüseyin; Kirmizigul, Suheyla

doi:10.1016/j.phymed.2017.11.009

Cited by 47 publications

(47 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The cell viability decreased to values of 64-71% at 72h of cultivation, probably due to the variety of phytochemical compounds present in the total extract of ivy leaves. The antitumor activity of saponins isolated from the aerial parts of Cephalaria species (from Turkey) was investigated using in vitro bioassays and showed that hederagenin was the most active compound against lung cancer cells [23].…”

Section: Antitumor Activitymentioning

confidence: 99%

Isolation and Characterization of Hederagenin from Hedera helix L. Extract with Antitumor Activity

Tatia¹,

Zălaru²,

Tarcomnicu³

et al. 2019

Rev. Chim.

View full text Add to dashboard Cite

Hederagenin, a saponin known for its therapeutic effect was isolated from leaves of Hedera helix. Hederagenin was obtained by repeated maceration of ground plant material in 95% (v/v) ethanol. The ethanolic extract was subjected to acid hydrolysis and purification with acetonitrile. Isolated hederagenin (IHe) was analyzed by HPLC-MS/MS and compared to standard hederagenin. In vitro cytotoxicity of IHe was tested in a culture of fibroblast cells from NCTC clone L929 cell line by MTT assay, using dioscin as positive control. For the determination of IHe antitumor effect, in vitro tests were performed in a culture of human cervix carcinoma Hep-2 cells cultivated in the presence of different concentrations of sample for 48 h. IHe was biocompatible in the range of concentrations 2-200 mg/mL, in NCTC cell culture. The compound showed cell cytotoxicity in the concentration range of 100-400 mg/mL, in Hep-2 cell culture, revealing its antitumor activity. These results demonstrated the possible use of hederagenin isolated from H. helix extract as an antitumor agent.

show abstract

Section: Antitumor Activitymentioning

confidence: 99%

Isolation and Characterization of Hederagenin from Hedera helix L. Extract with Antitumor Activity

Tatia¹,

Zălaru²,

Tarcomnicu³

et al. 2019

Rev. Chim.

View full text Add to dashboard Cite

show abstract

“…Our study showed that the hydroalcoholic extract of C. americericum has a very low oral route toxicity [39] and a high intraperitoneal route toxicity (LD 50% = 175 mg / kg of body weight). This intraperitoneal toxicity can be explained by saponins (Chrysantheline A & B) presence in the plant extracts [40,41,42]. Fortunately, this plant is used traditionally by decoction and drink.…”

Section: Discussionmentioning

confidence: 99%

Relevance of Chrysanthellum americanum (L.) Vatke Extracts in Rat Liver Protection

Guenné

Ouattara

Meda³

et al. 2019

IJBcRR

View full text Add to dashboard Cite

Aims: To assess the relevance of Chrysanthellum americanum (L.) Vatke extracts in rat liver protection. Place and Duration of Study: Laboratory of Biochemistry and Applied Chemistry (LABIOCA), also in Laboratory of Department of Medicine and Traditional Pharmacopoeia (MEPHATRA-PH) of Institute for Research in Health Sciences (IRSS/CNRST) of Burkina Faso between July 2014 and August 2015. Study Design: Polyphenolic extract of Chrysanthellum americanum- in vivo liver protection- in vivo liver intoxication- liver necrosis parameters analysis, histopathology analysis, in vivo and in vitro antioxidant assay. Background: Chrysanthellum americanum L. (Vatke) is a medicinal plant well known for its flavonoids and saponins richness, but also for its strong antioxidant potential and use traditionally for liver disease treatment. Methodology: In vivo, anti hepatotoxicity effects of Chrysanthellum americanum was evaluated using CCl4 as hepatotoxic agent. Also, acute toxicities were determined using standards methods, serum parameters of liver injury using Cypress Diagnostics kits and histopathology analysis using Mayer's haematoxylin- eosin-phloxine coloration method. For in vitro tests, malondialdehyde and thiobarbituric acid method were used in lipid peroxidation assessment and the ABTS method in Trolox Equivalent Antioxidant Capacity assessing. Results: Result showed that the crude extract of C. americericum has a very low oral toxicity but, in intraperitoneal route this extract presented a high toxicity (LD50= 175 mg / kg of body weight). Histopathology micrograph indicated reduction in number of necrotic cells induced by CCl4. This beneficial action was confirmed by reduction in serum transaminases and malondialdehyde (22.68 ± 0.68 mmol MDA/ g of liver weight). In vitro antioxidant capacities, this plant extract presented a result of 35.01 ± 0.26 % and 42.01 ± 0.26 mg TE/ g respectively in LPO and TEAC. Conclusion: Given our results, our research confirms that Chrysanthellum americanum extracts have in vivo physiological impact and benefits in traditional medicine for specific care of liver diseases.

show abstract

“…de Groot et al report that α-tomatine is a promising saponin in that it demonstrates quite low hemolytic activity; however, it has a high affinity for cholesterol [75]. Sarykayha et al report that saponins with oleanolic acid in the aglycone group cause significant hemolysis compared to those with a hederagenin aglycone: Aristatoside C, cephoside A and davisianoside B exhibited significant hemolytic activity on human blood cells when administered at 500 µg/mL, the respective values being 89.80%, 67.70% and 66.17% [76].…”

Section: Toxicology and Safety Of Saponinsmentioning

confidence: 99%

Saponins as Modulators of the Blood Coagulation System and Perspectives Regarding Their Use in the Prevention of Venous Thromboembolic Incidents

2020

View full text Add to dashboard Cite

Saponins comprise a heterogenous group of chemical compounds containing a triterpene or steroid aglycone group and at least one sugar chain. They exist as secondary metabolites, occurring frequently in dicotyledonous plants and lower marine animals. Plant saponin extracts or single saponins have indicated antiplatelet and anticoagulant activity. Venous thromboembolism (VTE), including deep venous thrombosis and pulmonary embolism, is a multifactorial disease influenced by various patient characteristics such as age, immobility, previous thromboembolism and inherited thrombophilia. This mini-review (1) evaluates the current literature on saponins as modulators of the coagulation system, (2) discusses the impact of chemical structure on the modulation of the coagulation system, which may further provide a basis for drug or supplement design, (3) examines perspectives of their use in the prevention of VTE. It also describes the molecular mechanisms of action of the saponins involved in the prevention of VTE.

show abstract

Immunomodulatory, hemolytic and cytotoxic activity potentials of triterpenoid saponins from eight Cephalaria species

Cited by 47 publications

References 35 publications

Isolation and Characterization of Hederagenin from Hedera helix L. Extract with Antitumor Activity

Isolation and Characterization of Hederagenin from Hedera helix L. Extract with Antitumor Activity

Relevance of Chrysanthellum americanum (L.) Vatke Extracts in Rat Liver Protection

Saponins as Modulators of the Blood Coagulation System and Perspectives Regarding Their Use in the Prevention of Venous Thromboembolic Incidents

Contact Info

Product

Resources

About