Immunopolarization-regulated 3D printed-electrospun fibrous scaffolds for bone regeneration

Liu, Xingzhi; Chen, Mimi; Luo, Junchao; Zhao, Huan; Zhou, Xichao; Gu, Qianqun; Yang, Huilin; Zhu, Xuesong; Cui, Wenguo; Shi, Qin

doi:10.1016/j.biomaterials.2021.121037

Cited by 116 publications

(62 citation statements)

References 49 publications

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“…Furthermore, BMP-2 protein can also regulate the transition of macrophages from the pro-inflammatory M1phenotype to the anti-inflammatory M2 phenotype [ [33] , [34] , [35] , [36] ]. Previous studies by us and others have demonstrated that M2 macrophages can inhibit inflammation and osteoclast differentiation by secreting anti-inflammatory factor IL-10, and secrete VEGF-A and TGF-β to promote angiogenesis and differentiation of MSC into osteoblasts, so as to promote osteogenesis [ [33] , [34] , [35] , [36] , [37] ].…”

Section: Introductionmentioning

confidence: 99%

Bio-clickable mussel-inspired peptides improve titanium-based material osseointegration synergistically with immunopolarization-regulation

Sun

Huang

Zhao

et al. 2022

Bioactive Materials

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Upon the osteoporotic condition, sluggish osteogenesis, excessive bone resorption, and chronic inflammation make the osseointegration of bioinert titanium (Ti) implants with surrounding bone tissues difficult, often lead to prosthesis loosening, bone collapse, and implant failure. In this study, we firstly designed clickable mussel-inspired peptides (DOPA-N3) and grafted them onto the surfaces of Ti materials through robust catechol-TiO 2 coordinative interactions. Then, two dibenzylcyclooctyne (DBCO)-capped bioactive peptides RGD and BMP-2 bioactive domain (BMP-2) were clicked onto the DOPA-N3-coated Ti material surfaces via bio-orthogonal reaction. We characterized the surface morphology and biocompatibility of the Ti substrates and optimized the osteogenic capacity of Ti surfaces through adjusting the ideal ratios of BMP-2/RGD at 3:1. In vitro, the dual-functionalized Ti substrates exhibited excellent promotion on adhesion and osteogenesis of mesenchymal stem cells (MSCs), and conspicuous immunopolarization-regulation to shift macrophages to alternative (M2) phenotypes and inhibit inflammation, as well as enhancement of osseointegration and mechanical stability in osteoporotic rats. In summary, our biomimetic surface modification strategy by bio-orthogonal reaction provided a convenient and feasible method to resolve the bioinertia and clinical complications of Ti-based implants, which was conducive to the long-term success of Ti implants, especially in the osteoporotic or inflammatory conditions.

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Section: Introductionmentioning

confidence: 99%

Bio-clickable mussel-inspired peptides improve titanium-based material osseointegration synergistically with immunopolarization-regulation

Sun

Huang

Zhao

et al. 2022

Bioactive Materials

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“…Due to its low melting temperature, PCL is a suitable material to be printed using extrusion-based additive manufacturing [ 22 ]. To improve bioactivity, different concentrations of hydroxyapatite (HA) and tricalcium phosphate (TCP) have been added to PCL scaffolds [ 23 , 24 , 25 , 26 , 27 , 28 ]. This improves both the mechanical and hydrophilicity properties and accelerates mineralisation [ 29 , 30 , 31 , 32 , 33 , 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

Novel 3D Bioglass Scaffolds for Bone Tissue Regeneration

et al. 2022

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The design of scaffolds with optimal biomechanical properties for load-bearing applications is an important topic of research. Most studies have addressed this problem by focusing on the material composition and not on the coupled effect between the material composition and the scaffold architecture. Polymer–bioglass scaffolds have been investigated due to the excellent bioactivity properties of bioglass, which release ions that activate osteogenesis. However, material preparation methods usually require the use of organic solvents that induce surface modifications on the bioglass particles, compromising the adhesion with the polymeric material thus compromising mechanical properties. In this paper, we used a simple melt blending approach to produce polycaprolactone/bioglass pellets to construct scaffolds with pore size gradient. The results show that the addition of bioglass particles improved the mechanical properties of the scaffolds and, due to the selected architecture, all scaffolds presented mechanical properties in the cortical bone region. Moreover, the addition of bioglass indicated a positive long-term effect on the biological performance of the scaffolds. The pore size gradient also induced a cell spreading gradient.

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“…Sci. 2022, 12,191 2 of 14 Ouyang et al [10] and Seyed et al [11] have investigated the influence of pore size on the hydromechanical properties of porous scaffolds, as well as the correlation with cell response and bone regeneration. They describe a scaffold with pores the size of 650 µm making use of titanium material.…”

Section: Introductionmentioning

confidence: 99%

“…They describe a scaffold with pores the size of 650 µm making use of titanium material. Xingzhi Liu [12] have used the same pore size; however, they utilized a poly(L-lactic acid) electrospun microfibrous structure. Zamani et al [13] have studied the distribution of FSS in scaffolds with different pore sizes: 300, 600, and 900 µm.…”

Section: Introductionmentioning

confidence: 99%

“…In this work, a comparative analysis is carried out to find the most adequate turbulence method for the design of a 3D scaffold by FDM 3D printing techniques [12,29]. For this, two biocompatible materials of similar densities were selected which are commonly used in FDM additive manufacturing type (polyetherimide "PEI", ULTEM 1010 biocompatible and polylactic acid "PLA" materials); a geometry was also selected based on the FDM's own layer-by-layer extrusion.…”

Section: Introductionmentioning

confidence: 99%

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A Comparative Study of Turbulence Methods Applied to the Design of a 3D-Printed Scaffold and the Selection of the Appropriate Numerical Scheme to Simulate the Scaffold for Tissue Engineering

Suffo

López-Marín

2021

Applied Sciences

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Current commercial software tools implement turbulence models on computational fluid dynamics (CFD) techniques and combine them with fluid-structural interaction (FSI) techniques. There are currently a great variety of turbulence methods that are worth investigating through a comparative study in order to delineate their behavior on scaffolds used in tissue engineering and bone regeneration. Additive manufacturing (AM) offers the opportunity to obtain three-dimensional printed scaffolds (3D scaffolds) that are designed respecting morphologies and that are typically used for the fused deposition model (FDM). These are typically made using biocompatible and biodegradable materials, such as polyetherimide (PEI), ULTEM 1010 biocompatible and polylactic acid (PLA). Starting from our own geometric model, simulations were carried out applying a series of turbulence models which have been proposed due to a variety of properties, such as permeability, speed regime, pressures, depressions and stiffness, that in turn are subject to boundary conditions based on a blood torrent. The obtained results revealed that the detached eddy simulation (DES) model shows better performance for the use of 3D scaffolds in its normal operating regime. Finally, although the results do not present relevant differences between the two materials used in the comparison, the prototypes simulated in PEI ULTEM 1010 do not allow their manufacture in FDM for the required pore size. The printed 3D scaffolds of PLA reveal an elastic behavior and a rigidity that are similar to other prototypes of ceramic composition. Prototypes made of PLA reveal unpredictable variability in pore and layer size which are very similar to cell growth itself and difficult to keep constant.

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Immunopolarization-regulated 3D printed-electrospun fibrous scaffolds for bone regeneration

Cited by 116 publications

References 49 publications

Bio-clickable mussel-inspired peptides improve titanium-based material osseointegration synergistically with immunopolarization-regulation

Bio-clickable mussel-inspired peptides improve titanium-based material osseointegration synergistically with immunopolarization-regulation

Novel 3D Bioglass Scaffolds for Bone Tissue Regeneration

A Comparative Study of Turbulence Methods Applied to the Design of a 3D-Printed Scaffold and the Selection of the Appropriate Numerical Scheme to Simulate the Scaffold for Tissue Engineering

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