Immunoproteomic analysis of secretory proteins ofAspergillus fumigatus with specific IGE immunoreactivity

Gautam, Poonam; Madan, Taruna; Gade, W. N.; Sarma, P. Usha

doi:10.1007/bf02912905

Cited by 6 publications

(5 citation statements)

References 15 publications

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“…3wcf proteins were extracted from Afu (strain 285, isolated from the sputum of a patient with ABPA visiting Vallabhbhai Patel Chest Institute, Delhi, India) as described previously [23], for performing Afu ‐specific IgG ELISA, Afu ‐specific IgE ELISA, 2‐DE and 2‐D‐specific IgE immunoblotting.…”

Section: Methodsmentioning

confidence: 99%

“…Earlier, we had reported a comparative two‐dimensional gel electrophoresis (2‐DE) and immunoblot analysis of Afu culture filtrates (first‐, second‐ and third‐week culture filtrate) and third‐week culture filtrate (3wcf) showed a larger number of specific IgE immunoreactive proteins [23]. Hence, in the present study, 3wcf proteins of Afu were used to identify novel allergens using pooled sera of Afu ‐sensitized asthmatics and their allergenic potential was also studied in ABPA patients to establish the candidate allergens, which could be explored further for diagnostic applications in patients with allergic aspergillosis including ABPA.…”

Section: Introductionmentioning

confidence: 99%

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Identification of novel allergens of Aspergillus fumigatus using immunoproteomics approach

Gautam

Sundaram

Madan

et al. 2007

Clin Experimental Allergy

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The immunoproteomic approach applied to the analysis of culture filtrate proteins resulted in the identification of several candidate allergens, many of them novel, contributing to the catalogue of Afu allergenic proteins, which would facilitate improved serodiagnosis for allergic aspergillosis. In addition, the immunoreactivity of these proteins observed among the patients with ABPA may be potentially useful for its serodiagnosis and opens up further opportunities for the development of personalized immunotherapeutics for patients with ABPA.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of novel allergens of Aspergillus fumigatus using immunoproteomics approach

Gautam

Sundaram

Madan

et al. 2007

Clin Experimental Allergy

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“…Therefore, the clinical picture is generally accompanied with an elevated total IgE level and Aspergillus -specific elevated IgE and IgG levels [ 115 ]. Such anti- Aspergillus antibody profiles of ABPA patients (and of A. fumigatus sensitized asthmatics) have been analyzed in a number of immunoproteomic studies [ 116 – 119 ]. Gautam et al [ 116 ], for example, studied the IgE immunoreactivity of A. fumigatus proteins by 2D gel electrophoresis followed by immunoblotting of 3-week old culture filtrate proteins with sera from A. fumigatus -sensitized asthmatics and ABPA patients.…”

Section: Introductionmentioning

confidence: 99%

Interference of Aspergillus fumigatus with the immune response

et al. 2014

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Aspergillus fumigatus is a saprotrophic filamentous fungus and also the most prevalent airborne fungal pathogen of humans. Depending on the host’s immune status, the variety of diseases caused by A. fumigatus ranges from allergies in immunocompetent hosts to life-threatening invasive infections in patients with impaired immunity. In contrast to the majority of other Aspergillus species, which are in most cases nonpathogenic, A. fumigatus features an armory of virulence determinants to establish an infection. For example, A. fumigatus is able to evade the human complement system by binding or degrading complement regulators. Furthermore, the fungus interferes with lung epithelial cells, alveolar macrophages, and neutrophil granulocytes to prevent killing by these immune cells. This chapter summarizes the different strategies of A. fumigatus to manipulate the immune response. We also discuss the potential impact of recent advances in immunoproteomics to improve diagnosis and therapy of an A. fumigatus infection.

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“…Protease secretion is under the control of NCR mediated by the transcriptional regulator, PrtT, which was shown to not be essential for virulence when neutropenic and cortisone acetate-treated mice models of pulmonary aspergillosis (Bergmann et al ., 2009; Sharon et al ., 2009). Similar to C. albicans , proteases modulate host immune responses (Behnsen et al ., 2010) and are thought to contribute to A. fumigatus virulence by facilitating host tissue degradation and invasion (Gautam et al ., 2006; Balenga et al ., 2015; Kogan et al ., 2004). Deletion of the alkaline serine protease alp1/aspf13 led to significantly reduced virulence in nasally infected neutropenic mice (Kolatukuddy et al ., 1993), although a more recent study showed that disruption of alp1 had no significant effect on virulence, when the strain was administered intranasally, in a cortisone acetate mouse model (Behnsen et al ., 2010).…”

Section: Nitrogen Catabolite Metabolism In Human Pathogenic Fungimentioning

confidence: 99%

Overview of carbon and nitrogen catabolite metabolism in the virulence of human pathogenic fungi

Ries

Beattie

Cramer

et al. 2017

Molecular Microbiology

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SummaryIt is estimated that fungal infections, caused most commonly by Candida albicans, Aspergillus fumigatus and Cryptococcus neoformans, result in more deaths annually than malaria or tuberculosis. It has long been hypothesized the fungal metabolism plays a critical role in virulence though specific nutrient sources utilized by human pathogenic fungi in vivo has remained enigmatic. However, the metabolic utilisation of preferred carbon and nitrogen sources, encountered in a host niche-dependent manner, is known as carbon catabolite and nitrogen catabolite repression (CCR, NCR), and has been shown to be important for virulence. Several sensory and uptake systems exist, including carbon and nitrogen sourcespecific sensors and transporters, that allow scavenging of preferred nutrient sources. Subsequent metabolic utilisation is governed by transcription factors, whose functions and essentiality differ between fungal species. Furthermore, additional factors exist that contribute to the implementation of CCR and NCR. The role of the CCR and NCR-related factors in virulence varies greatly between fungal species and a substantial gap in knowledge exists regarding specific pathways. Further elucidation of carbon and nitrogen metabolism mechanisms is therefore required in a fungal species-and animal model-specific manner in order to screen for targets that are potential candidates for anti-fungal drug development.

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Immunoproteomic analysis of secretory proteins ofAspergillus fumigatus with specific IGE immunoreactivity

Abstract: ABSTRACT

Cited by 6 publications

References 15 publications

Identification of novel allergens of Aspergillus fumigatus using immunoproteomics approach

Identification of novel allergens of Aspergillus fumigatus using immunoproteomics approach

Interference of Aspergillus fumigatus with the immune response

Overview of carbon and nitrogen catabolite metabolism in the virulence of human pathogenic fungi

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