Immunosensor for the ultrasensitive and quantitative detection of bladder cancer in point of care testing

Chuang, Cheng-Hsin; Du, Yi; Wu, Ting; Chen, Cheng Ho; Lee, Da Huei; Chen, Shih Min; Huang, Ting-Chi; Wu, Hsun Pei; Shaikh, Muhammad Omar

doi:10.1016/j.bios.2015.12.103

Cited by 53 publications

(35 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Other authors proposed the use of AD5933 to make low-cost biosensor systems for applications in DNA and protein sensing (van Grinsven et al, 2010), detection of bladder cancer (Chuang et al, 2016) and avian flu virus (Wang et al, 2015). Kamat et al (2014) discussed a sensor for non-invasive blood glucose analysis; Ferreira et al proposed an application for total body composition (Ferreira et al, 2010) and body fluid distribution analysis (Ferreira et al, 2011);Park et al (2006) discussed the implementation of a sensor system to monitor civil infrastructures (particularly for loosening bolts in a bolt-jointed structure and detection of corrosion in an aluminum beam); Durante et al (2016) developed a sensor to be used for real-time monitoring to detect fraud in milk composition.…”

Section: Introductionmentioning

confidence: 99%

Electrical impedance spectroscopy (EIS) for biological analysis and food characterization: a review

Grossi

Riccò

2017

J. Sens. Sens. Syst.

312

160

View full text Add to dashboard Cite

Abstract. Electrical impedance spectroscopy (EIS), in which a sinusoidal test voltage or current is applied to the sample under test to measure its impedance over a suitable frequency range, is a powerful technique to investigate the electrical properties of a large variety of materials. In practice, the measured impedance spectra, usually fitted with an equivalent electrical model, represent an electrical fingerprint of the sample providing an insight into its properties and behavior. EIS is used in a broad range of applications as a quick and easily automated technique to characterize solid, liquid, semiliquid, organic as well as inorganic materials. This paper presents an updated review of EIS main implementations and applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Electrical impedance spectroscopy (EIS) for biological analysis and food characterization: a review

Grossi

Riccò

2017

J. Sens. Sens. Syst.

312

160

View full text Add to dashboard Cite

show abstract

“…An impedance-based immunosensor was designed and fabricated to quantitatively detect Galectin-1 (Gal-1) protein, a biomarker for bladder cancer [79]. To detect bladder cancer, alumina nanoparticles conjugated with the Gal-1 antibody (nanoprobes) were first trapped onto the gold microelectrode patterned on the immunosensor chip surface using programmable dielectrophoretic (DEP) manipulations.…”

Section: Impedance-based Detectionmentioning

confidence: 99%

Microdevices for Non-Invasive Detection of Bladder Cancer

et al. 2017

View full text Add to dashboard Cite

Bladder cancer holds the record for the highest lifetime cost on a per-patient basis. This is due to high recurrence rates, which necessitate invasive and costly long-term evaluation methods such as cystoscopy and imaging. Microfluidics is emerging as an important approach to contribute to initial diagnosis and follow-up, by enabling the precise manipulation of biological samples. Specifically, microdevices have been used for the isolation of cells or genetic material from blood samples, sparking significant interest as a versatile platform for non-invasive bladder cancer detection with voided urine. In this review, we revisit the methods of bladder cancer detection and describe various types of markers currently used for evaluation. We detail cutting-edge technologies and evaluate their merits in the detection, screening, and diagnosis of bladder cancer. Advantages of microscale devices over standard methods of detection, as well as their limitations, are provided. We conclude with a discussion of criteria for guiding microdevice development that could deepen our understanding of prognoses at the level of individual patients and the underlying biology of bladder cancer development. Collectively, the development and widespread application of improved microfluidic devices for bladder cancer could drive treatment breakthroughs and establish widespread, tangible outcomes on patients' long-term survival.

show abstract

“…al. that can operate in a frequency range of 1 KHz−100 KHz as shown in Figure 3 [28]. It is based on an AD5933 integrated circuit (IC) where the response signal from the impedance is sampled by the on-board ADC and a discrete Fourier transform (DFT) and communicated to a digital microcontroller through an InterIntegrated Circuit (I 2 C) interface.…”

Section: Impedance Measurementsmentioning

confidence: 99%

“…This approach also poses challenges when the bioreceptor needs to be immobilized specifically on the electrode surface. One way of improving physical immobilization on electrode surfaces is by using AC electro kinetic techniques like dielectrophoresis (DEP) to trap the bioreceptor on the electrode surface and some examples are illustrated in Figure 5 [28,[36][37][38]. DEP can be defined as the translational motion of a dielectric particle or biological cell in a suspended medium under the influence of a non-uniform AC electric field which acts on the particle driving it towards/against the direction of the electric field maxima.…”

Section: Immobilization Strategiesmentioning

confidence: 99%

“…Recently, Chuang et. al developed an impedance immunosensor utilizing an interdigitated microelectrode array for the detection of bladder cancer biomarker, Galectin-1 (Gal-1) [28]. To improve sensitivity and immobilization efficiency, they have utilized nanoprobes (Gal-1 antibodies conjugated to alumina nanoparticles through silane modification) that are trapped on the microelectrode surface using programmable dielectrophoretic manipulations.…”

Section: Non-faradaic Impedance Immunosensorsmentioning

confidence: 99%

See 1 more Smart Citation