Immunosensors

Karunakaran, C.; Manickam, Pandiaraj; Santharaman, Paulraj

doi:10.1016/b978-0-12-803100-1.00004-9

Cited by 23 publications

(5 citation statements)

References 50 publications

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“…Moreover, using flow or batch injection analysis speeds up the test time and improves the throughput of samples [25]. Since Ags and Abs are not intrinsically electroactive, a label is usually linked to the Ag or the Ab to produce the electrochemical signal [26]. Usually, a labeled secondary Ab is used to generate the signal, in order to avoid labeling Abs for each specific Ag.…”

Section: Platform Design For Ad Biosensorsmentioning

confidence: 99%

Electrochemical Biosensors as Potential Diagnostic Devices for Autoimmune Diseases

et al. 2019

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An important class of biosensors is immunosensors, affinity biosensors that are based on the specific interaction between antibodies and antigens. They are classified in four classes based on the type of employed transducer: electrochemical, optical, microgravimetric, and thermometric and depending on the type of recognition elements, antibodies, aptamers, microRNAs and recently peptides are integrating parts. Those analytical devices are able to detect peptides, antibodies and proteins in various sample matrices, without many steps of sample pretreatment. Their high sensitivity, low cost and the easy integration in point of care devices assuring portability are attracting features that justify the increasing interest in their development. The use of nanomaterials, simultaneous multianalyte detection and integration on platforms to form point-of-care devices are promising tools that can be used in clinical analysis for early diagnosis and therapy monitoring in several pathologies. Taking into account the growing incidence of autoimmune disease and the importance of early diagnosis, electrochemical biosensors could represent a viable alternative to currently used diagnosis methods. Some relevant examples of electrochemical assays for autoimmune disease diagnosis developed in the last several years based on antigens, antibodies and peptides as receptors were gathered and will be discussed further.

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Section: Platform Design For Ad Biosensorsmentioning

confidence: 99%

Electrochemical Biosensors as Potential Diagnostic Devices for Autoimmune Diseases

et al. 2019

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“…Amperometric immunosensors represent a highly developed biosensing field that has led to the appearance of several commercialized products [ 24 ]. This kind of sensors can measure the current change at a constant voltage signifying the occurrence of a redox reaction on the analyte, because the resulting current on the sensing electrode surface is proportional to the analyte concentration [ 25 ].…”

Section: Electrochemical Immunosensors With Different Signalsmentioning

confidence: 99%

Nanomaterials for Electrochemical Immunosensing

Pan

Yun

et al. 2017

Sensors

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Electrochemical immunosensors resulting from a combination of the traditional immunoassay approach with modern biosensors and electrochemical analysis constitute a current research hotspot. They exhibit both the high selectivity characteristics of immunoassays and the high sensitivity of electrochemical analysis, along with other merits such as small volume, convenience, low cost, simple preparation, and real-time on-line detection, and have been widely used in the fields of environmental monitoring, medical clinical trials and food analysis. Notably, the rapid development of nanotechnology and the wide application of nanomaterials have provided new opportunities for the development of high-performance electrochemical immunosensors. Various nanomaterials with different properties can effectively solve issues such as the immobilization of biological recognition molecules, enrichment and concentration of trace analytes, and signal detection and amplification to further enhance the stability and sensitivity of the electrochemical immunoassay procedure. This review introduces the working principles and development of electrochemical immunosensors based on different signals, along with new achievements and progress related to electrochemical immunosensors in various fields. The importance of various types of nanomaterials for improving the performance of electrochemical immunosensor is also reviewed to provide a theoretical basis and guidance for the further development and application of nanomaterials in electrochemical immunosensors.

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“…Immunosensor is a biorecognition-based sensor that detects biomarkers selectively because of the binding between antigen and antibody, forming a stable immune-complex (Karunakaran et al , 2015). Immunosensors are categorized depending on the types of their transducing systems into optical, piezoelectric and electrochemical (Majer-Baranyi et al , 2016; Pohanka, 2018; Lu et al , 2019).…”

Section: Introductionmentioning

confidence: 99%

Developing an electrochemical immunosensor for early diagnosis of hepatocellular carcinoma

Al-Shami

Oweis

Al-Fandi

2021

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Purpose This paper aims to report on the development of a novel electrochemical amperometric immunosensor to diagnose early hepatocellular carcinoma (HCC) by detecting the Midkine (MDK) biomarker. Design/methodology/approach Anti-Midkine antibodies were immobilized covalently through carbodiimides chemistry on carbon screen-printed electrodes modified with carboxylated multi-walled carbon nanotubes. The development process was characterized using cyclic voltammetry, electrochemical impedimetric spectroscopy, Fourier transform infrared spectroscopy and atomic force microscopy. Differential pulse voltammetry was used to investigate the immunosensor performance in detecting MDK antigen within the concentration range of 1 pg/ml to 100 ng/ml. Findings MDK immunosensor exhibited high sensitivity and linearity with a detection limit of 0.8 pg/ml and a correlation coefficient of 0.99. The biosensor also demonstrated high selectivity, stability and reproducibility. Originality/value The developed MDK immunosensor could be a promising tool to diagnose HCC and reduce the number of related deaths.

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Immunosensors

Cited by 23 publications

References 50 publications

Electrochemical Biosensors as Potential Diagnostic Devices for Autoimmune Diseases

Electrochemical Biosensors as Potential Diagnostic Devices for Autoimmune Diseases

Nanomaterials for Electrochemical Immunosensing

Developing an electrochemical immunosensor for early diagnosis of hepatocellular carcinoma

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