Immunostaining Methods for Frozen and Paraffin Sections

Mepham, B. L.; Britten, K. J. M.

doi:10.1007/978-94-009-0739-3_12

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2001

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Cited by 8 publications

(2 citation statements)

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“…Sections from one block from each case were stained immunohistochemically using a streptavidin–biotin method, 12 with microwave antigen retrieval using pH 6 citrate buffer 13 before application of the primary antibody. The primary antibodies used were: QBEND‐10 (CD34, Novocastra, Peterborough, UK), and bcl‐2 (Dako, Ely, UK).…”

Section: Methodsmentioning

confidence: 99%

Expression of CD34 and bcl‐2 in phyllodes tumours, fibroadenomas and spindle cell lesions of the breast

Moore

Lee

2001

Histopathology

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The combined expression of CD34 and bcl-2 suggests that fibroadenomas, phyllodes tumours and pseudoangiomatous hyperplasia may arise from long-lived bcl-2-positive mesenchymal cells in the breast in a manner similar to that proposed for solitary fibrous tumours. The absence of CD34 staining in spindle cell carcinomas is of potential diagnostic value in the distinction from malignant phyllodes tumours in difficult cases.

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Section: Methodsmentioning

confidence: 99%

Expression of CD34 and bcl‐2 in phyllodes tumours, fibroadenomas and spindle cell lesions of the breast

Moore

Lee

2001

Histopathology

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“…Vessel counting was performed on formalin-fixed, paraffin-embedded sections stained for CD34 using a streptavidin-biotin complex method 19 with microwave pretreatment 20 . A pilot study had shown that ulex europaeus lectin stained tumour as well as endothelium, making vessel counting impossible; and antibody to von Willebrand factor stained tumour necrosis and collagen, making counting difficult in some tumours.…”

Section: E S S E L C O U N T I N Gmentioning

confidence: 99%