Immunostimulation Effect of Jellyfish Collagen

Abstract

Certain edible large jellyfishes belonging to the order Rhizostomeae are consumed in large quantities in China and Japan. The exumbrella part of the edible jellyfish Stomolophus nomurai was cut and soaked in dilute hydrochloric acid solution (pH 3.0) for 12 h, and heated at 121 degrees C for 20 min. The immunostimulation effects of the jellyfish extract were examined. The jellyfish extract enhanced IgM production of human hybridoma HB4C5 cells 34-fold. IgM and IgG production of human peripheral blood lymphocytes (PBL) were also accelerated, 2.8- and 1.4-fold respectively. Moreover, production of interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha by human PBL was stimulated 100- and 17-fold respectively. Collagenase treatment inactivated the immunostimulation activity of the jellyfish extract. In addition, purified collagen from bovine Achilles' tendon accelerated IgM production of hybridoma cells. These facts mean that collagen has an immunostimulation effect, and that the active substance in jellyfish extract is collagen.

Immunostimulation Effects of Yellowtail Heart Extractsin Vitroandin Vivo

“…7) Hence, yellowtail heart extract may also affect translation as well as transcription activity. It is also possible that stimulated cell proliferation due to yellowtail heart extract results in accelerated IgM production, as for jellyfish collagen, 6) but this is unlikely because the cultivation period of HB4C5 cells with yellowtail heart extract was no more than 6 h in this study.…”

“…4) Also, we have found that jellyfish extract stimulates Ig production by HB4C5 cells and Ig and cytokine production by human PBLs. 6) Because jellyfish extract treated with a collagenase lost immunostimulation activity and purified collagen from bovine Achilles' tendon accelerated IgM production of hybridoma cells, it is probable that the active substance in jellyfish extract is collagen. We examined the mode of action of jellyfish collagen and found that it stimulated not only transcription activity, but also translation activity accelerating Ig production.…”

Coastal Zones Ecosystem Services

“…Jellyfi sh are believed to contain collagen, which moisturise the skin, and to cure rheumatoid arthritis and bronchitis (Hay 2006 ;Sugahara et al 2006 ). They provide Aequorin and Obelin, which are green fl uorescent proteins that are for instance used as biomarkers in biomedical research.…”

Immunological and Toxinological Responses to Jellyfish Stings

“…This concept is supported by disparate lines of evidence. Whilst the nematocyst-derived venom of the edible jellyfish Nemopilema (Stomolophus) nomurai causes cardiovascular depression in experimental animals [11] and may cause death in humans [12], the collagen extracted from the exumbrella of this jellyfish enhanced IgM, IgG, interferon and tumour necrosis factor production by human lymphocytes [13, 14]. This source of collagen also enhanced inflammatory cytokine secretion, antibody secretion and population changes in immune cells [15].…”