Immunotherapy for Visceral Leishmaniasis: Ability of Factors Produced during Anti-leishmania Responses of Skin Test Positive Adults to Inhibit Peripheral Blood Mononuclear Cell Activities Associated with Visceral Leishmaniasis

Holaday, B J

doi:10.1590/s0074-02761999000100013

Cited by 3 publications

(4 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Low levels of endogenous IL-10 se-cretion accompanied by high levels of antigen specific IL-10 secretion might result in anergy to Leishmania antigens alone, as observed in other VL patients (Carvalho et al 1981, Haldar et al 1983. The data also support the hypothesis that IL-10 secretion is regulated, in part, by Leishmania antigens (Holaday 1999). When endogenous IL-10 secretion reached approximately 0.5 ng/ml, addition of Leishmania antigens appeared to trigger a regulatory mechanism which decreased both endogenous and antigen specific IL-10 secretion.…”

supporting

confidence: 77%

“…Elevated levels of interleukin-10 (IL) secretion occur in some visceral leishmaniasis patients (Karp et al 1993, Ghalib et al 1993, Holaday et al 1993). Cultures of peripheral blood mononuclear cells (PBMC) isolated from VL patients have shown that elevation of either endogenous or antigen specific IL-10 secretion or both can occur (Gasim et al 1998, Holaday 1999. Increased IL-10 secretion is thought to be an important element in the immunopathology of VL since it is a potent inhibitor of macrophage activation in general and IFN-γ secretion in particular (Mossman & Moore 1991, Howard & O'Garra 1992.…”

mentioning

confidence: 99%

“…The correlation of endogenous and antigen specific IL-10 secretion with antigen specific IFN-γ secretion was determined for PBMC from 19 acute and five treated VL patients using methods described previously (Holaday 1999). PBMC from one of the patients were assayed twice, once three days after initiation of treatment and a second time seven days later.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Role of CD8+ T cells in endogenous interleukin-10 secretion associated with visceral leishmaniasis

Holaday

2000

Mem. Inst. Oswaldo Cruz

Self Cite

View full text Add to dashboard Cite

This study examined the role and source of endogenous interleukin-10 (IL) secretion in visceral leishmaniasis (VL). The amounts of endogenous and Leishmania specific IL-10 and interferon-gamma (IFN) secreted by peripheral blood mononuclear cells (PBMC) from VL patients were compared. The correlation coefficient between endogenous IL-10 secretion and Leishmania specific IFN-gamma was -0.77, suggesting a major role for endogenous IL-10 secretion in VL. The effects of CD4+ and CD8+ T cell clones, isolated from a treated VL patient, on IL-10 secretion were assayed by mixing the clones with autologous, inactivated PBMC. The CD8+ clones mediated increased levels of IL-10 secretion in the presence of PBMC alone suggesting that CD8+ T cells may mediate endogenous IL-10 secretion

show abstract

supporting

confidence: 77%

mentioning

confidence: 99%

See 1 more Smart Citation

Role of CD8+ T cells in endogenous interleukin-10 secretion associated with visceral leishmaniasis

Holaday

2000

Mem. Inst. Oswaldo Cruz

Self Cite

View full text Add to dashboard Cite

show abstract

“…In mouse model studies, it has been observed that although Tregs are essential to induce tolerance and prevent autoimmune diseases, they are down-regulated during an infectious episode to facilitate elimination of the infection like tuberculosis, Leishmania and malaria [16,17]. Increased number of Tregs has been associated with poor prognosis in breast, colorectal and ovarian cancers [18].…”

Section: Tregs and Diseasementioning

confidence: 99%

T-Regulatory Cells: The Recently Recognized Players of Immunomodulation

Av¹,

Hl²

2014

J Stem Cell Res Ther

View full text Add to dashboard Cite

Recently a subpopulation of T cells known as regulatory T cells (Tregs) have been identified to play important role of immunomodulation in autoimmune diseases, cancer and also in transplantation immunobiology. Tregs have been identified as biomarkers for graft function status. We have generated Tregs in vitro and also used them in kidney transplantation. We have found their efficacy in safe minimization of immunosuppression in kidney transplantation. Tregs are likely to form an integral part of cell therapy which will completely change the field of clinical transplantation. However there is a wide chasm between basic cell biologists and immunobiologists (who understand the significance of Tregs) and clinicians (who do not appreciate the bedside role of Tregs). This review article discusses the genesis of Tregs, their identification markers and their diagnostic and therapeutic role in transplantation immunobiology.

show abstract

Deletion of MIF gene from live attenuated LdCen−/− parasites enhances protective CD4+ T cell immunity

Fiuza

Gannavaram

Gaze

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Vaccination with live attenuated Leishmania parasites such as centrin deleted Leishmania donovani (LdCen−/−) against visceral leishmaniasis has been reported extensively. The protection induced by LdCen−/− parasites was mediated by both CD4+ and CD8+ T cells. While the host immune mediators of protection are known, parasite determinants that affect the CD4+ and CD8+ T cell populations remain unknown. Parasite encoded inflammatory cytokine MIF has been shown to modulate the T cell differentiation characteristics by altering the inflammation induced apoptosis during contraction phase in experimental infections with Leishmania or Plasmodium. Neutralization of parasite encoded MIF either by antibodies or gene deletion conferred protection in Plasmodium and Leishmania studies. We investigated if the immunogenicity and protection induced by LdCen−/− parasites is affected by deleting MIF genes from this vaccine strain. Our results showed that LdCen−/−MIF−/− immunized group presented higher percentage of CD4+ and CD8+ central memory T cells, increased CD8+ T cell proliferation after challenge compared to LdCen−/− immunization. LdCen−/−MIF−/− immunized group presented elevated production of IFN-γ+ and TNF-α+ CD4+ T cells concomitant with a reduced parasite load in spleen and liver compared to LdCen−/−group following challenge with L. infantum. Our results demonstrate the role of parasite induced factors involved in protection and long-term immunity of vaccines against VL.

show abstract

Immunotherapy for Visceral Leishmaniasis: Ability of Factors Produced during Anti-leishmania Responses of Skin Test Positive Adults to Inhibit Peripheral Blood Mononuclear Cell Activities Associated with Visceral Leishmaniasis

Abstract: The course of human Leishmania chagasi infections appears to be determined by the balance between type 1 (T1) CD4+ and CD8+ T suppressor (Ts)

Cited by 3 publications

References 23 publications

Role of CD8+ T cells in endogenous interleukin-10 secretion associated with visceral leishmaniasis

Role of CD8+ T cells in endogenous interleukin-10 secretion associated with visceral leishmaniasis

T-Regulatory Cells: The Recently Recognized Players of Immunomodulation

Deletion of MIF gene from live attenuated LdCen−/− parasites enhances protective CD4+ T cell immunity

Contact Info

Product

Resources

About