IMP3 Immunoreactivity is More Sensitive Than AMACR in Detecting Dysplastic Epithelium and Early Adenocarcinoma in Barrett Esophagus

Gadara, Manoj; González, Mercedes; Cartun, Richard W.; Ligato, Saverio

doi:10.1097/pai.0000000000000319

Cited by 8 publications

(7 citation statements)

References 18 publications

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“…Studies have found that IGF2BP3 is closely associated with the occurrence of leukemia ( 36 ), atypical hyperplasia of Barrett’s esophagus ( 37 ), pancreatic duct neoplasia ( 38 ) and atypical endometriosis ( 39 ), etc. These evidences indicate that IGF2BP3 may play an important role in the occurrence of tumors.…”

Section: Discussionmentioning

confidence: 99%

Integration Analysis of m6A Related Genes in Skin Cutaneous Melanoma and the Biological Function Research of the SPRR1B

et al. 2021

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Melanoma has gradually entered the public view because of its high morbidity and rising prevalence rate, which is a serious threat to human life and health. Recently, N6-methyladenine (m6A) modification has been increasingly confirmed as a potential role in the development of tumogenesis. The purpose of this study is to explore the role and function of m6a-related regulators in the development of melanoma disease at the molecular, cellular and clinical levels through bioinformatics and traditional experiments. We screened and validated differential expression genes (DEGs) in m6A regulators via the GEO, GTEx, TCGA database. The biological processes and signaling pathway involved by DEGs were improved by constructing bioinformational methods such as PPI, GO enrichment, KEGG enrichment, GSEA enrichment, and immune infiltration analysis. And then, we explored the biological function of the key gene, SPRR1B, through cell invasion, migration, infiltration, and tissue chips. The gene IGF2BP3 which was differentially expressed in m6A regulatory factor gene was screened. The results of the enrichment analysis are significantly enriched in the biological processes and pathways of the skin barrier, epidermal differentiation, cytoskeleton, lymphocyte migration and other pathways, pointing to the direction of tumor immunity and tumor metastasis. Tumor immune-related genes YTHDC1, YTHDC2 and ALKBH5 were found. Knock SPRR1B reduction group had a significantly lower invasive ability, the ability to migrate. Nomogram prediction model shows that SPRR1B increased, expressing a worse prognosis. For this purpose, the relationship between m6A regulatory factor and melanoma progression was explored. At the same time, it was found that the abnormal up-regulated expression of SPRR1B before metastasis would lead to poor prognosis of melanoma. SPRR1B promotes the proliferation, invasion and migration of human melanoma cells.

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Section: Discussionmentioning

confidence: 99%

Integration Analysis of m6A Related Genes in Skin Cutaneous Melanoma and the Biological Function Research of the SPRR1B

et al. 2021

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“…Beyond indicating the capability of IGF2BP3 to recapitulate a fetal-like phenotype, these evidences suggest a putative role of IGF2BP3 in tumorigenesis since the de novo expression of RBP in adult tissues apparently provides a favorable context for the emergence of neoplastic lesions. Accordingly, IGF2BP3 is detectable in some premalignant human lesions, including dysplasia in Barrett esophagus (Gadara et al, 2017), pancreatic intraductal neoplasia (Wang et al, 2015), and atypical endometriosis (Vercellini et al, 2013); in addition, many tumor types upregulate IGF2BP3 compared to normal tissue counterparts (Figure 2).…”

Section: Igf2bp3mentioning

confidence: 99%

IGF2BP3 From Physiology to Cancer: Novel Discoveries, Unsolved Issues, and Future Perspectives

Mancarella

Scotlandi

2020

Front. Cell Dev. Biol.

118

126

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RNA network control is a key aspect of proper cellular homeostasis. In this context, RNA-binding proteins (RBPs) play a major role as regulators of the RNA life cycle due to their capability to bind to RNA sequences and precisely direct nuclear export, translation/degradation rates, and the intracellular localization of their target transcripts. Alterations in RBP expression or functions result in aberrant RNA translation and may drive the emergence and progression of several pathological conditions, including cancer. Among the RBPs, insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) is of particular interest in tumorigenesis and tumor progression. This review highlights the molecular mechanisms underlying the oncogenic functions of IGF2BP3, summarizes the therapeutic potential related to its inhibition and notes the fundamental issues that remain unanswered. To fully exploit IGF2BP3 for tumor diagnosis and therapy, it is crucial to dissect the mechanisms governing IGF2BP3 re-expression and to elucidate the complex interactions between IGF2BP3 and its target mRNAs as normal cells become tumor cells.

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“…Many studies have shown that IGF2BP3 has a promoting role in the development of tumours. Accordingly, high expression of IGF2BP3 has been detected in some precancerous human diseases, such as abnormal hyperplasia in Barrett's oesophagus and tumours in the pancreas 22,23 . IGF2BP3 is a highly conserved gene with few mutations, and its stability is favourable for its use as a tumour marker.…”

Section: Discussionmentioning

confidence: 99%

Atractylodes macrocephalaIII suppresses EMT in cervical cancer by regulating IGF2BP3 through ETV5

Wang,

Meng,

Wang

et al. 2024

J Cellular Molecular Medi

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Atractylodes macrocephala III (ATL III), with anti‐inflammatory and antitumor effects, is the main compound of Atractylodes macrocephala. Whether ATL III has an effect on cervical cancer and the specific mechanism are still unclear. Here, we investigated the effects of ATL III on cervical cancer cells at different concentrations and found that ATL III downregulates insulin‐like growth factor 2 mRNA‐binding protein 3 (IGF2BP3), which was found to be highly expressed in cervical cancer tissue by RNA‐Seq. In this study, we found that ATL III promotes apoptosis and regulates epithelial–mesenchymal transition (EMT) in cervical cancer cells (HeLa and SiHa cells) and that IGF2BP3 is a common target gene of ATL III in HeLa and SiHa cells. The expression level of IGF2BP3 in cervical cancer cells was proportional to their migration and invasion abilities. This was verified by transfection of cells with a small interfering RNA and an IGF2BP3 overexpression plasmid. After ATL III treatment, the migration and invasion abilities of cervical cancer cells were obviously reduced, but these effects were attenuated after overexpression of IGF2BP3. In addition, the transcription factor IGF2BP3 was predicted by the JASPAR system. After intersection with our sequencing results, we verified the promotional effect of ETV5 (ETS translocation variant 5) on IGF2BP3 and found that ALT III inhibited ETV5. In general, our research showed that ATL III inhibits the migration and invasion of cervical cancer cells by regulating IGF2BP3 through ETV5.

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IMP3 Immunoreactivity is More Sensitive Than AMACR in Detecting Dysplastic Epithelium and Early Adenocarcinoma in Barrett Esophagus

Abstract: IMP3 has a similar specificity, but a better sensitivity, intensity, and extent of reactivity in comparison with AMACR, and may be used as an alternative to AMACR, in support of the diagnosis of BE-dysplasia and EAC.

Cited by 8 publications

References 18 publications

Integration Analysis of m6A Related Genes in Skin Cutaneous Melanoma and the Biological Function Research of the SPRR1B

Integration Analysis of m6A Related Genes in Skin Cutaneous Melanoma and the Biological Function Research of the SPRR1B

IGF2BP3 From Physiology to Cancer: Novel Discoveries, Unsolved Issues, and Future Perspectives

Atractylodes macrocephalaIII suppresses EMT in cervical cancer by regulating IGF2BP3 through ETV5

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