Impact of copper on in-vitro biomineralization, drug release efficacy and antimicrobial properties of bioactive glasses

Chitra, S.; Bargavi, P.; Balasubramaniam, M.; Chandran, R. Riju; Balakumar, S.

doi:10.1016/j.msec.2019.110598

Cited by 60 publications

(37 citation statements)

References 74 publications

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“…In this context, Mn ions have been shown to upregulate osteogenic marker genes and to improve extracellular matrix (ECM) formation when added to cell culture settings 7,13‐17 . There are several other ions with distinct therapeutic activity, which are interesting for application in BTE such as zinc (Zn) and copper (Cu) 8,18 . Zn stimulates osteoblasts toward protein synthesis, regulates the mineralization of the ECM and influences the expression of osseous marker genes such as osteopontin ( OPN ) and osteocalcin ( OCN ) 19‐22 .…”

Section: Introductionmentioning

confidence: 99%

Effect of manganese, zinc, and copper on the biological and osteogenic properties of mesoporous bioactive glass nanoparticles

Westhauser

Wilkesmann

Nawaz

et al. 2020

J Biomedical Materials Res

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Mesoporous bioactive glass nanoparticles (MBGNs) have demonstrated promising properties for the local delivery of therapeutically active ions with the aim to improve their osteogenic properties. Manganese (Mn), zinc (Zn), and copper (Cu) ions have already shown promising pro‐osteogenic properties. Therefore, the concentration‐dependent impact of MBGNs (composition in mol%: 70 SiO2, 30 CaO) and MBGNs containing 5 mol% of either Mn, Zn, or Cu (composition in mol%: 70 SiO2, 25 CaO, 5 MnO/ZnO/CuO) on the viability and osteogenic differentiation of human marrow‐derived mesenchymal stromal cells (BMSCs) was assessed in this study. Mn‐doped MBGNs (5Mn‐MBGNs) showed a small “therapeutic window” with a dose‐dependent negative impact on cell viability but increasing pro‐osteogenic features alongside increasing Mn concentrations. Due to a constant release of Zn, 5Zn‐MBGNs showed good cytocompatibility and upregulated the expression of genes encoding for relevant members of the osseous extracellular matrix during the later stages of cultivation. In contrast to all other groups, BMSC viability increased with increasing concentration of Cu‐doped MBGNs (5Cu‐MBGNs). Furthermore, 5Cu‐MBGNs induced an increase in alkaline phosphatase activity. In conclusion, doping with Mn, Zn, or Cu can enhance the biological properties of MBGNs in different ways for their potential use in bone regeneration approaches.

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Section: Introductionmentioning

confidence: 99%

Effect of manganese, zinc, and copper on the biological and osteogenic properties of mesoporous bioactive glass nanoparticles

Westhauser

Wilkesmann

Nawaz

et al. 2020

J Biomedical Materials Res

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“…It also has the ability to increase resistance to dissolution [ 127 , 128 , 129 ] with some antibacterial activity. Copper and gallium, on the other hand, improve biomineralization and amplify antimicrobial properties [ 67 , 128 , 130 ]. Wu et al suggested the doping of MBG foams with europium (1–5 mol.%) to fabricate luminescent scaffolds for biolabeling and clinical imaging applications without altering the bioactivity [ 131 ].…”

Section: Discussionmentioning

confidence: 99%

Mesoporous Bioactive Glasses Cytocompatibility Assessment: A Review of In Vitro Studies

et al. 2021

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Thanks to their high porosity and surface area, mesoporous bioactive glasses (MBGs) have gained significant interest in the field of medical applications, in particular, with regards to enhanced bioactive properties which facilitate bone regeneration. The aim of this article is to review the state of the art regarding the biocompatibility evaluation of MBGs and provide a discussion of the various approaches taken. The research was performed using PubMed database and covered articles published in the last five years. From a total of 91 articles, 63 were selected after analyzing them according to our inclusion and exclusion criteria. In vitro methodologies and techniques used for biocompatibility assessment were investigated. Among the biocompatibility assessment techniques, scanning electron microscopy (SEM) has been widely used to study cell morphology and adhesion. Viability and proliferation were assessed using different assays including cell counting and/or cell metabolic activity measurement. Finally, cell differentiation tests relied on the alkaline phosphatase assay; however, these were often complemented by specific bimolecular tests according to the exact application of the mesoporous bioactive glass. The standardization and validation of all tests performed for MBG cytocompatibility is a key aspect and crucial point and should be considered in order to avoid inconsistencies, bias between studies, and unnecessary consumption of time. Therefore, introducing standard tests would serve an important role in the future assessment and development of MBG materials.

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“…SiO 2 (45%), P 2 O 5 (6%), CaO (24.5%), and Na 2 O (24.5%) sources were utilized to synthesize bioactive glass by sol–gel method. Synthesis protocol was briefly reported in our earlier report (Chitra, Bargavi, Balasubramaniam, RijuChandran, & Balakumar, 2020). Further to that, the prepared bioactive glasses were annealed at various temperatures such 600°C for 3 hr heat‐treated bioactive material designated as 600‐3h, similarly 600°C for 2 hr named as 600‐2h, step annealing was carried out to decompose the nitrate content while extending the heat treatment at 550°C for 3 hr along with 600°C for 3 hr and likewise 550°C for 2 hr with 600°C for 3 hr, the corresponding materials were designated as 550‐3h and 550‐2h, respectively.…”

Section: Methodsmentioning

confidence: 99%

Thermal treatment stimulus on erythrocyte compatibility and hemostatic behavior of one‐dimensional bioactive nanostructures

Chitra

Bargavi

et al. 2020

J Biomedical Materials Res

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This study identifies the role and significance of heat treatment parameters on blood compatibility and hemostatic performances. Bioactive nanomaterials step annealed at 550 and 600°C enhances the biocompatibility due to the liberation of nitrate content. This also develops the anisotropic structures such as needle‐like and rod‐like appearances that positively improve the erythrocyte compatibility. Different stable crystalline phases such as NaCaPO4, Na2Ca2Si3O9, and Na1.8Ca1.1Si6O14 were observed for all the bioactive materials, whereas in the case of 800°C, phase transition of Na3CaPSiO7 was perceived along with P2O5. Alternatively, morphology varied from cubical (600°C) to rod‐like (step annealing) and further turned toward flake‐like (800°C) structures. Step‐annealing process decomposed the nitrate groups as well as maintained the glass network without altering the crystalline phases. As a result, bioactive nanomaterials subjected to step annealing at 550°C along with 600°C exhibited superior compatibility with erythrocytes. Bioglass heat treated at 800°C revealed incredible blood clotting efficacy, in which Ca2+ ions initiated the thrombotic effect, blood components were concentrated due to the effect of calcium, and enhances the hemostatic performance. Bioactive glass annealed below 800°C facilitates the biocompatibility, subsequently encourage bone ingrowths at in vivo. Bioglass‐800°C inspired the fibrin formation and induced clot as a hemostat to control hemorrhage.

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Impact of copper on in-vitro biomineralization, drug release efficacy and antimicrobial properties of bioactive glasses

Cited by 60 publications

References 74 publications

Effect of manganese, zinc, and copper on the biological and osteogenic properties of mesoporous bioactive glass nanoparticles

Effect of manganese, zinc, and copper on the biological and osteogenic properties of mesoporous bioactive glass nanoparticles

Mesoporous Bioactive Glasses Cytocompatibility Assessment: A Review of In Vitro Studies

Thermal treatment stimulus on erythrocyte compatibility and hemostatic behavior of one‐dimensional bioactive nanostructures

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