Impact of different extraction methods on the quality of Dipteryx alata extracts

Introduction:The objective of this study was to compare three capsaicin extraction methods: Shoxlet, Ultrasound-assisted Extraction (UAE), and Shaker-assisted Extraction (SAE) from Habanero pepper, CNPH 15.192.Materials and Methods:The different parameters evaluated were alcohol degree, time extraction, and solid–solvent ratio using response surface methodology (RSM).Results:The three parameters found significant (p < 0.05) were for UAE and solvent concentration and extraction time for SAE. The optimum conditions for the capsaicin UAE and SAE were similar 95% alcohol degree, 30 minutes and solid–liquid ratio 2 mg/mL. The Soxhlet increased the extraction in 10–25%; however, long extraction times (45 minutes) degraded 2% capsaicin.Conclusion:The extraction of capsaicin was influenced by extraction method and by the operating conditions chosen. The optimized conditions provided savings of time, solvent, and herbal material. Prudent choice of the extraction method is essential to ensure optimal yield of extract, thereby making the study relevant and the knowledge gained useful for further exploitation and application of this resource.SUMMARY Habanero pepper, line CNPH 15.192, possess capsaicin in higher levels when compared with others speciesHigher levels of ethanolic strength are more suitable to obtain a higher levels of capsaicinBox-Behnken design indicates to be useful to explore the best conditions of ultrasound assisted extraction of capsaicin. Abbreviations used: Nomenclature UAE: Ultrasound-assisted Extraction; SAE: Shaker-assisted Extraction.

“…Among these, multivariate analysis associated with RSM is efficient due to its versatility, ability to assess multiple factors, ease of performance, and robustness. [ 13 14 ]…”

Section: Introductionmentioning

confidence: 99%

Novel approaches to extraction methods in recovery of capsaicin from habanero pepper (CNPH 15.192)

Martins¹,

Borges²,

Ribeiro³

et al. 2017

“…Several extraction methods exist, including those employing (i) heating maceration, (ii) refluxing, (iii) Soxhlet extraction, (iv) supercritical fluids. [ 1 2 3 4 5 ] Same this techniques generally require long extraction time, organic solvents toxics that may have potential negative on human health and environment. [ 6 7 8 ] Among these technics, the ultrasound-assisted extraction (UEA) and percolations associate with chemometrics technics as response surface methodology (RSM) are commonly used in pharmaceutical industries.…”

Section: Introductionmentioning

confidence: 99%

Technical aspects on production of fluid extract from Brosimum gaudichaudii Trécul roots

Martins¹,

Pascoa²,

Paula³

et al. 2015

Instruction:Despite the increased use of Brosimum gaudichaudii roots as raw material on medicine to treatment of vitiligo, there are not studies that showing the impact of unit operations on the quality and standardized of the extract of B. gaudichaudii. The quality of the herbal extract is essential to ensure the safety and efficacy of pharmaceutical product. Due the medical and commercial importance, this study aimed to evaluate the impact of the extraction method (ultrasound or percolation) on the quality of herbal extract and optimize the extraction of psoralen and 8-methoxypsoralen (8-MOP) from B. gaudichaudii.Materials and Methods:The extraction recovery was evaluate by high-performance liquid chromatography (C8 reverse phase column and acetonitrile: Water 45:55 and flow rate 0.6 mL/min). The extraction was performed by ultrasound-assisted extraction (UEA) or percolation using a Box-Behnken design.Results:From both chemical markers (psoralen and bergapten), the optimal conditions for the UEA were an extraction time of 25 min, the mean particle size of 100 μm, and an ethanol: Water ratio of 55:45 (v/v).Conclusion:The extraction by percolation revealed that ethanol 55% was more efficient than ethanol 80% to extract psoralen and bergapten.

“…Medicinal plants are important as raw material to new medicines, however that this fact becomes reality is necessary studies to prove their efficacy, safety and source of raw material. [ 1 2 ] Often the chemical and medicinal potential of the plant is discarded because there is no standardization of raw material, generating results not accurate, with low reproducibility. Now there are advanced techniques to aid in the standardization of raw materials, e.g.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of extraction method on the chemical composition in Apeiba tibourbou Aubl′s extracts

Martins

Conceição

2015

Background:The extraction method of bioactive compounds is an important step in the manufacturing of herbal medicines, because secondary metabolites with therapeutic potential are usually found in small quantities in plant materials.Objective:Due the potential of Apeiba tibourbou Aubl, this study aimed to evaluate the impact of the extraction method on the quality of herbal extract and optimize the extraction of fatty acid, rosmarinic (Ra) and caffeic (Ca) acid from A. tibourbou.Materials and Methods:Determinations of residual moisture (Rm), proteins (Pt), lipids (Lp), total fiber (Tf), and carbohydrate (Cy) were performed in triplicate samples according assessment of antioxidant capacity. Extraction of fatty acids was carried out by two different methods: (i) By shoxlet and (ii) bligh and dyer. The optimized conditions were determined by surface response methodology (RSM), and the criterion of desirability was the maximum extraction of Ra and Ca.Results:The method of bligh and dyer was able to extraction more total Lp than the shoxlet. However, the extraction of fatty acid was different for the two methods. The optimized conditions to extract RA and Ca was calculated by RSM, 42°C, 30% (alcohol degree) and 24 min, this conditions maximize simultaneously the extraction of Ca (0, 04%) and Ry (1.89),Conclusion:It was observed that the extraction method alters the chemical composition of extract, and it is possible to extract Ca and Ra from A. tibourbou's leaves using ultrasound-assisted extraction.