Impact of different hold time before addition of platelet additive solution on the in vitro quality of apheresis platelets

Ringwald, Juergen; Haager, B; Krex, Daniel; Zimmermann, Robert; Strasser, Erwin; Antoon, M.; Schrijver, Eddy De; Eckstein, Reinhold

doi:10.1111/j.1537-2995.2006.00826.x

Cited by 11 publications

(16 citation statements)

References 27 publications

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“…The storage medium is normally composed of a mixture of plasma (generally 20%‐40%) and PAS (60‐80%). With apheresis technology, hyperconcentrated PLT units can be collected with either manual or automated addition of PAS without additional centrifugation or washing . This technique has been used to produce plasma‐reduced PCs with plasma concentrations as low as 5% .…”

Section: Discussionmentioning

confidence: 99%

In vitro variables of buffy coat–derived platelet concentrates with residual plasma of down to 10% are stably maintained in new‐generation platelet additive solutions

et al. 2015

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Section: Discussionmentioning

confidence: 99%

In vitro variables of buffy coat–derived platelet concentrates with residual plasma of down to 10% are stably maintained in new‐generation platelet additive solutions

et al. 2015

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“…The very low values for PLT P‐selectin expression for both kinds of products throughout storage are the most surprising finding in our study. In two former studies using exactly the same method for determination of P‐selectin expression, we had obtained also low values for P‐selectin expression for PLTs collected as HPSs using TA and stored in PAS‐IIIM 6,9 . The values in these studies, however, ranged notably higher with means of 6% up to 17% during 7‐day storage.…”

Section: Discussionmentioning

confidence: 74%

“…There have been little data published on the impact of a prolonged hold time as HPS on PLT quality. When comparing a 2‐ and 8‐hour hold time, our group found an enhanced metabolism of glucose during the hold time as HPS, but we were able to detect no significant differences during subsequent 7‐day storage in the in vitro variables that are associated with in vivo viability and activation of PLTs 9 . Only one group has addressed the question whether immediate or 2‐hour‐delayed PAS addition might result in an increased PLT activation 10 .…”

mentioning

confidence: 62%

“…In our former study comparing a 2‐ and 8‐hour hold time, we detected significantly lower glucose and higher lactate concentrations associated with lower pH values in the units with the 8‐hour hold time 9 . This indicated that the PLTs may have used anaerobic glycolysis for energy production during the 6‐hour prolonged hold time as HPSs.…”

Section: Discussionmentioning

confidence: 83%

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Effects of immediate or delayed addition of platelet additive solution on the in vitro quality of apheresis platelets

et al. 2011

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“…Parameters used to measure the viability of donated platelets include the swirl phenomenon, pH, p-selectin and, in vivo, post transfusion platelet increment and corrected count increment (CCI). Several compounds in platelet additive solutions such as citrate, acetate, phosphate, potassium, magnesium and glucose, have all been shown to be important [2,3].…”

Section: Introductionmentioning

confidence: 99%

Investigating the effect of a platelet additive solution on apheresis platelet and fibrin network ultrastructure

Pretorius

Crookes

Oberholzer

et al. 2010

Transfusion and Apheresis Science

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a b s t r a c tIn thrombotic events and diseases such as cancer, HIV/AIDS, dysfibrinogenaemia, as well as acute incidents (e.g. burn wounds), ultrastructure of platelets and fibrin networks change. In the current study, we compare the ultrastructure of platelets and fibrin networks of apheresis platelets stored in citrated human plasma (CP) and in a first-generation platelet additive solution (PAS) (T-Sol), to that of fresh donor plasma (FP). Eighteen apheresis platelet donors donated platelets on Trima Ò -Accel™ V5.2 and V5.1 cell separators. Six collections were stored for five days in autologous citrated plasma (CP); six collections were stored in 40% citrated human plasma and 60% PAS solution (CP/PAS) controlled, for the duration of storage, at a constant temperature (22 ± 2°C) with continuous flat-bed agitation; and six collections were stored in conditions uncontrolled for temperature and without continuous agitation. On days 1, 3 and 5, equal volumes of human thrombin were mixed with platelets collected in either CP or CP/PAS to form a coagulum (fibrin network containing platelet aggregates), followed by preparation for scanning electron microscopy. Results were compared with platelets and fibrin networks in FP. Typically, in FP, platelet aggregates with smooth membranes and pseudopodia are seen and fibrin networks arrange to form major, thick fibers and scattered, minor, thin fibers. On day 1, in CP and in all CP/PAS units, platelet ultrastructure compared well to that of FP, although the fibrin fibers were denser, with the minor fibers forming a matted layer over the major fibers. On day 3, in platelet units uncontrolled for temperature and without continuous agitation during storage, some platelet aggregates in CP/PAS showed typical apoptotic morphology, with shrinkage and membrane damage, but comparable fibrin networks were present. On day 5 however, in those units where storage conditions were uncontrolled and where the pH had decreased to below 6.4, no platelet aggregates were seen and fibrin was arranged into short, lumpy masses with no separate major or minor fibrin fibers visible. In those units stored at 22°C with continuous flat-bed agitation, where pH was maintained >7.0, ultrastructure of platelets and fibrin network in CP/PAS was typical and similar to FP and CP at the end of five days of storage. Examining platelet and fibrin network ultrastructure may be useful, in addition to conventional laboratory analysis, in assessing the viability and potential clinical efficacy of platelets for transfusion and could play a role in the evaluation of new generation platelet additive solutions.

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Impact of different hold time before addition of platelet additive solution on the in vitro quality of apheresis platelets

Cited by 11 publications

References 27 publications

In vitro variables of buffy coat–derived platelet concentrates with residual plasma of down to 10% are stably maintained in new‐generation platelet additive solutions

In vitro variables of buffy coat–derived platelet concentrates with residual plasma of down to 10% are stably maintained in new‐generation platelet additive solutions

Effects of immediate or delayed addition of platelet additive solution on the in vitro quality of apheresis platelets

Investigating the effect of a platelet additive solution on apheresis platelet and fibrin network ultrastructure

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