Impact of microwave irradiation on enzymatic activity at constant bulk temperature is enzyme-dependent

Microwave-assisted delivery of an anticancer drug to cancer cells

“…S1 † for representative temperature and microwave power proles for cultures in the microwave reactor). As demonstrated previously, [3][4][5] we were able to maintain the temperature of the culture within AE1-2 C of setpoint values.…”

“…Trypsin, but not a-amylase or phosphatase, activity was signicantly increased by exposure to 10 W microwaves at constant temperature. 3,4 Low power microwaves also appear to slightly perturb membrane properties in PC-3 prostate cancer cells, without causing apoptosis or necrosis. 5 Escherichia coli growth is also affected by exposure to non-lethal microwaves, though the effect is transient (unpublished results).…”

Microwave Irradiation of PC3 Cells at Constant Culture Temperature Alters the Incorporation of BODIPY into Cells and Reduction of MTT

“…In order to maintain the culture temperature, the cultures were irradiated with microwave while the temperature, as measured in real-time by a fibre optic temperature probe (Figure 1), was maintained at 37 AE 2 8C through cooling. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 Maintenance of temperature of PC3 cultures exposed to microwave As demonstrated by us previously, [7,8] our system maintains temperature of the cell suspension in the CEM microwave reactor within 1 or 2 degrees of the setpoint, here 37 8C. In the present study, temperatures of all the PC3 cell suspensions were continually monitored over the duration of the experiments.…”

“…As demonstrated by us previously,, our system maintains temperature of the cell suspension in the CEM microwave reactor within 1 or 2 degrees of the setpoint, here 37 °C. In the present study, temperatures of all the PC3 cell suspensions were continually monitored over the duration of the experiments.…”

“…[7] The enzymatic activity of trypsin is significantly increased by exposure to 2.45 GHz microwave irradiation while maintaining temperature of the reaction mixture via simultaneous cooling. Interestingly though, the enzymatic activity of amylase and phosphatase are not affected by exposure to microwave under the same conditions, [8] indicating specificity of the effect.…”