Impact of overexpression of 9-cis-epoxycarotenoid dioxygenase on growth and gene expression under salinity stress

Martínez‐Andújar, Cristina; Martínez-Pérez, Ascensión; Ferrández‐Ayela, Almudena; Albacete, Alfonso; Martínez-Melgarejo, Purificación A.; Dodd, Ian C.; Thompson, Andrew J.; Pérez-Pérez, José Manuel; Pérez‐Alfocea, Francisco

doi:10.1016/j.plantsci.2019.110268

Cited by 35 publications

(34 citation statements)

References 75 publications

(93 reference statements)

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“…Our data indicate that ABA accumulation triggered by salt perception in roots increases over time with almost comparable levels in both genotypes, whereas in leaves it is higher in "Spagnolo" than "Bianco Avorio" throughout the salt exposure period. This increase in root ABA levels may support higher root elongation observed in both genotypes, which was significant only in "Spagnolo", similarly to the increased total root length induced by ABA in salt-stressed tomato [44].…”

Section: Discussionmentioning

confidence: 52%

Physiological, Biochemical, and Metabolic Responses to Short and Prolonged Saline Stress in Two Cultivated Cardoon Genotypes

Docimo

Stefano

Cappetta

et al. 2020

Plants

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Cultivated cardoon is a multipurpose crop with adaptability to limiting environments. Two genotypes (“Bianco Avorio” and “Spagnolo”) were comparatively characterized in response to short and prolonged 100 mM NaCl stress in hydroponics. Salt induced no growth variations between genotypes or symptoms of NaCl toxicity, but boosted ABA accumulation in roots and leaves. Both genotypes had high constitutive phenol content, whose major components were depleted upon 2 days of stress only in “Bianco Avorio”. Prolonged stress stimulated accumulation of proline, phenylpropanoids, and related transcripts, and non-enzymatic antioxidant activity. Decreased antioxidant enzymes activities upon short stress did not occur for APX in “Spagnolo”, indicating a stronger impairment of enzymatic defenses in “Bianco Avorio”. Nonetheless, H2O2 and lipid peroxidation did not increase under short and prolonged stress in both genotypes. Overall, the two genotypes appear to share similar defense mechanisms but, in the short term, “Bianco Avorio” depends mainly on non-enzymatic antioxidant phenylpropanoids for ROS scavenging, while “Spagnolo” maintains a larger arsenal of defenses. Upon prolonged stress, proline could have contributed to protection of metabolic functions in both genotypes. Our results provide cues that can be exploited for cardoon genetic improvement and highlight genotypic differences for breeding salinity tolerant varieties.

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Section: Discussionmentioning

confidence: 52%

Physiological, Biochemical, and Metabolic Responses to Short and Prolonged Saline Stress in Two Cultivated Cardoon Genotypes

Docimo

Stefano

Cappetta

et al. 2020

Plants

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“…In roots of soybean, some Calmodulin Binding Transcription Activator (GmCAMTAs) are important during early response to water stress and the overexpression of GmCAMTA12 in roots of Arabidopsis enhance drought survival [138]. Concerning to phytohormones, LeNCED1 overexpression, a key gene in ABA biosynthesis, improved salinity response [139] and the knockout of ABA 8'hydroxylase (OsABA8ox2), involved in ABA catabolism, enhanced drought tolerance [140]. In soybean, Salt induced1 (GmSIN1) overexpression induced GmNCED3 and GmRbhoB, improving salt tolerance, suggesting GmSIN1 gene could work as a modulator between ABA and ROS signaling [141].…”

Section: Signal Transduction and Stress-induced Gene Expressionmentioning

confidence: 99%

Root Involvement in Plant Responses to Adverse Environmental Conditions

et al. 2020

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Climate change is altering the environment in which plants grow and survive. An increase in worldwide Earth surface temperatures has been already observed, together with an increase in the intensity of other abiotic stress conditions such as water deficit, high salinity, heavy metal intoxication, etc., generating harmful conditions that destabilize agricultural systems. Stress conditions deeply affect physiological, metabolic and morphological traits of plant roots, essential organs for plant survival as they provide physical anchorage to the soil, water and nutrient uptake, mechanisms for stress avoidance, specific signals to the aerial part and to the biome in the soil, etc. However, most of the work performed until now has been mainly focused on aerial organs and tissues. In this review, we summarize the current knowledge about the effects of different abiotic stress conditions on root molecular and physiological responses. First, we revise the methods used to study these responses (omics and phenotyping techniques). Then, we will outline how environmental stress conditions trigger various signals in roots for allowing plant cells to sense and activate the adaptative responses. Later, we discuss on some of the main regulatory mechanisms controlling root adaptation to stress conditions, the interplay between hormonal regulatory pathways and the global changes on gene expression and protein homeostasis. We will present recent advances on how the root system integrates all these signals to generate different physiological responses, including changes in morphology, long distance signaling and root exudation. Finally, we will discuss the new prospects and challenges in this field.

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“…Notably, the significantly higher root biomass of Atlante under salinity (Figure 1b) would allow better access to water resources as it has been shown in open-field crops with modulated CK biosynthesis by transgenic rootstocks [29,56]. Furthermore, ABA sensitivity has been demonstrated to improve the water status in grafted tomato [14,57], pepper [24], and cucumber [25] plants under salinity. Thus, the coordinated modulation of ABA and CKs levels of pepper plants grafted onto Atlante would help to regulate stomatal aperture [58] and/or density [59], maintaining stomatal conductance (Figure 2f), transpiration rate (Figure 2g), and photosynthesis (Figure 2e) under salinity.…”

Section: Discussionmentioning

confidence: 78%

Contrasting Rootstock-Mediated Growth and Yield Responses in Salinized Pepper Plants (Capsicum annuum L.) Are Associated with Changes in the Hormonal Balance

Gálvez

Albacete

Martínez‐Andújar

et al. 2021

IJMS

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Salinity provokes an imbalance of vegetative to generative growth, thus impairing crop productivity. Unlike breeding strategies, grafting is a direct and quick alternative to improve salinity tolerance in horticultural crops, through rebalancing plant development. Providing that hormones play a key role in plant growth and development and stress responses, we hypothesized that rootstock-mediated reallocation of vegetative growth and yield under salinity was associated with changes in the hormonal balance. To test this hypothesis, the hybrid pepper variety (Capsicum annuum L. “Gacela F1”) was either non-grafted or grafted onto three commercial rootstocks (Creonte, Atlante, and Terrano) and plants were grown in a greenhouse under control (0 mM NaCl) and moderate salinity (35 mM NaCl) conditions. Differential vegetative growth versus fruit yield responses were induced by rootstock and salinity. Atlante strongly increased shoot and root fresh weight with respect to the non-grafted Gacela plants associated with improved photosynthetic rate and K+ homeostasis under salinity. The invigorating effect of Atlante can be explained by an efficient balance between cytokinins (CKs) and abscisic acid (ABA). Creonte improved fruit yield and maintained the reproductive to vegetative ratio under salinity as a consequence of its capacity to induce biomass reallocation and to avoid Na+ accumulation in the shoot. The physiological responses associated with yield stability in Creonte were mediated by the inverse regulation of CKs and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid. Finally, Terrano limited the accumulation of gibberellins in the shoot thus reducing plant height. Despite scion compactness induced by Terrano, both vegetative and reproductive biomass were maintained under salinity through ABA-mediated control of water relations and K+ homeostasis. Our data demonstrate that the contrasting developmental and physiological responses induced by the rootstock genotype in salinized pepper plants were critically mediated by hormones. This will be particularly important for rootstock breeding programs to improve salinity tolerance by focusing on hormonal traits.

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Impact of overexpression of 9-cis-epoxycarotenoid dioxygenase on growth and gene expression under salinity stress

Cited by 35 publications

References 75 publications

Physiological, Biochemical, and Metabolic Responses to Short and Prolonged Saline Stress in Two Cultivated Cardoon Genotypes

Physiological, Biochemical, and Metabolic Responses to Short and Prolonged Saline Stress in Two Cultivated Cardoon Genotypes

Root Involvement in Plant Responses to Adverse Environmental Conditions

Contrasting Rootstock-Mediated Growth and Yield Responses in Salinized Pepper Plants (Capsicum annuum L.) Are Associated with Changes in the Hormonal Balance

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