Impact of oxygen-calcium-generating and bone morphogenetic protein-2 nanoparticles on survival and differentiation of bone marrow-derived mesenchymal stem cells in the 3D bio-printed scaffold

Aghajanpour, Sareh; Esfandyari-Manesh, Mehdi; Ghahri, Tahmineh; Ghahremani, Mohammad Hossein; Atyabi, Fatemeh; Heydari, Mehdi; Motasadizadeh, Hamidreza; Dinarvand, Rassoul

doi:10.1016/j.colsurfb.2022.112581

Cited by 18 publications

(6 citation statements)

References 30 publications

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“…For example, extreme UV levels and pH changes may negatively affect cell viability. In contrast, suitable temperature and Ca 2+ concentration changes do not seem to adversely affect living cells (Aghajanpour et al, 2022), and relatively mild 4D conversion stimulates the mechanism to be more friendly to the host environment. Despite substantial progress in hydrogel-based 4D biomanufacturing, available cytocompatible materials capable of 4D conversion in a physiological environment are still limited.…”

Section: Challengesmentioning

confidence: 99%

“…Moreover, this injectable hybrid hydrogel has ideal rheological properties and in vivo self-coagulation ability to fill minor and irregularly shaped defects. Currently, bone morphogenic protein-2 nanoparticles (Aghajanpour et al, 2022) and notoginsenoside R1 (Wang, Yan, Lan, Wei, Zheng, Wu, Jaspers, Wu, Pathak) are incorporated into hydrogel systems as induction factors to enhance the MSCs' differentiation ability to repair bone defects.…”

Section: Steps and Classification Of Fourdimensional Bioprintingmentioning

confidence: 99%

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Bioprinting for bone tissue engineering

Kang

Zhang²,

Gao³

et al. 2022

Front. Bioeng. Biotechnol.

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The shape transformation characteristics of four-dimensional (4D)-printed bone structures can meet the individual bone regeneration needs, while their structure can be programmed to cross-link or reassemble by stimulating responsive materials. At the same time, it can be used to design vascularized bone structures that help establish a bionic microenvironment, thus influencing cellular behavior and enhancing stem cell differentiation in the postprinting phase. These developments significantly improve conventional three-dimensional (3D)-printed bone structures with enhanced functional adaptability, providing theoretical support to fabricate bone structures to adapt to defective areas dynamically. The printing inks used are stimulus-responsive materials that enable spatiotemporal distribution, maintenance of bioactivity and cellular release for bone, vascular and neural tissue regeneration. This paper discusses the limitations of current bone defect therapies, 4D printing materials used to stimulate bone tissue engineering (e.g., hydrogels), the printing process, the printing classification and their value for clinical applications. We focus on summarizing the technical challenges faced to provide novel therapeutic implications for bone defect repair.

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Section: Challengesmentioning

confidence: 99%

Section: Steps and Classification Of Fourdimensional Bioprintingmentioning

confidence: 99%

Bioprinting for bone tissue engineering

Kang

Zhang²,

Gao³

et al. 2022

Front. Bioeng. Biotechnol.

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“…Bioprinting, or the use of 3D printing technologies in concert with bioinks containing bioactive components, is a common technique utilized in tissue engineering due to its ability to create repeatable hierarchical structures that mimic physiological tissue [26][27][28]. The use of controlled release systems within 3D printed scaffolds has the capacity to further increase the relevance of the biochemical environment of the scaffolds [29]; however, the release kinetics of the particles may be influenced by the forces imposed on the particles during printing (for example, the shear forces imposed by extrusion through a needle), and diffusion through the scaffolding material may limit the bioactivity of the released growth factor [30][31][32]. Crosslinking of the biomaterial or hydrogel ink itself may also affect the release kinetics and the ability of loaded growth factors to diffuse through the scaffold, affecting the growth factor's interactions with incorporated cells.…”

Section: Introductionmentioning

confidence: 99%

Development of a Nanoparticle System for Controlled Release in Bioprinted Respiratory Scaffolds

Zimmerling,

Sunil,

Zhou

et al. 2024

JFB

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The use of nanoparticle systems for the controlled release of growth factors is a promising approach to mimicking of the biochemical environment of native tissues in tissue engineering. However, sustaining growth factor release inside an appropriate therapeutic window is a challenge, particularly in bioprinted scaffolds. In this study, a chitosan-coated alginate-based nanoparticle system loaded with hepatocyte growth factor was developed and then incorporated into bioprinted scaffolds. The release kinetics were investigated with a focus on identifying the impact of the chitosan coating and culture conditions. Our results demonstrated that the chitosan coating decreased the release rate and lessened the initial burst release, while culturing in dynamic conditions had no significant impact compared to static conditions. The nanoparticles were then incorporated into bioinks at various concentrations, and scaffolds with a three-dimensional (3D) structure were bioprinted from the bioinks containing human pulmonary fibroblasts and bronchial epithelial cells to investigate the potential use of a controlled release system in respiratory tissue engineering. It was found that the bioink loaded with a concentration of 4 µg/mL of nanoparticles had better printability compared to other concentrations, while the mechanical stability of the scaffolds was maintained over a 14-day culture period. The examination of the incorporated cells demonstrated a high degree of viability and proliferation with visualization of the beginning of an epithelial barrier layer. Taken together, this study demonstrates that a chitosan-coated alginate-based nanoparticle system allows the sustained release of growth factors in bioprinted respiratory tissue scaffolds.

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“…Utilizing GelMA together with ceramic materials such as hydroxyapatite nanoparticles and nanosilica can lead to the construction of a more favorable scaffold [16,17]. Alginate also has excellent properties such as high degradability, non-toxicity, and gel-forming ability [18,19]. Alginate can be modi ed to have different mechanical properties, making it suitable for a range of applications and an attractive option for tissue engineering applications [18].…”

Section: Introductionmentioning

confidence: 99%

3D printed core/shell scaffold based on nano/microspheric hydrogel for osteosarcoma anticancer delivery and bone regeneration

Ranjbaran,

Esfandyari-Manesh,

Yourdkhani

et al. 2024

Preprint

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One of the most common types of bone cancer is osteosarcoma. Currently a combination of therapies including surgery, chemotherapy, and radiation therapy is used. Bone defects, re-formation of the tumor, or remaining tumor cells after the surgery are the main challenges of osteosarcoma treatments. Scaffolds can be used to overcome the bone defects problem. In this study, we aim to fabricate a bilayer scaffold with the capacity of avoiding tumor recurrence and stimulating bone regeneration which brings a novel idea for osteosarcoma treatments. First, methotrexate was encapsulated in PLGA microspheres with 13.5% loading capacity. Then, coaxial extrusion-based 3D printer via a customized bilayer core-shell nozzle was employed to fabricate the scaffold. The implanted scaffold was printed by using gelatin methacrylol (GelMA) hydrogel containing methotrexate microspheres in the outer layer for anticancer drug delivery, and GelMA/alginate hydrogel containing nanohydroxyapatite and nanosilica in the inner layer for bone regeneration. The outer layer of the scaffold had rapidly degraded within 20 days and it played a great role in drug delivery and inhibiting the tumor cells’ growth. The inner layer with 4% nanosilica had slow degradation rate at about 50% in 60 days and it showed the highest mechanical strength with 225 kPa. Regarding osteogenesis property, ALP enzyme activity was increased considerably within 3 weeks. Also, significant increase in osteogenesis markers of RUNX2, OPN, and COL1A1 was observed. In addition to drug delivery at the tumor site, this bilayer scaffold could be a platform for the placement of healthy bone cells after drug delivery.

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Impact of oxygen-calcium-generating and bone morphogenetic protein-2 nanoparticles on survival and differentiation of bone marrow-derived mesenchymal stem cells in the 3D bio-printed scaffold

Cited by 18 publications

References 30 publications

Bioprinting for bone tissue engineering

Bioprinting for bone tissue engineering

Development of a Nanoparticle System for Controlled Release in Bioprinted Respiratory Scaffolds

3D printed core/shell scaffold based on nano/microspheric hydrogel for osteosarcoma anticancer delivery and bone regeneration

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