Impact of pathogen reduction technology and storage in platelet additive solutions on platelet function

Abstract

PLT properties were preserved in PRT-treated concentrates stored in PAS for 5 days. PASIIIM provides a better preservation of adhesive and cohesive functions of PRT-PCs than PASIII for 7 days of storage. The relevance of in vitro data deserves further clinical investigations.

In vitro study of platelet function confirms the contribution of the ultraviolet B (UVB) radiation in the lesions observed in riboflavin/UVB‐treated platelet concentrates

“…A change of ATP consumption rate is another hypothesis for the increased glycolytic flux in riboflavin/UVB‐treated PLTs. Increased expression of P‐selectin and lower ATP content were reported in riboflavin/UVB‐treated PLTs . We also found an increased expression of CD62P, active GPIIbIIIa at the surface of the PLTs, and an increased adhesion to fibrinogen indicating that the riboflavin/UVB‐treated PLTs were somehow activated upon treatment.…”

“…The results from this study reveal that the riboflavin/UVB treatment significantly impacts the metabolism of PLTs as evidenced by higher glucose consumption and lactate production rates. This phenomenon has been observed by other groups in Mirasol‐treated PLTs . It has been hypothesized that the increased anaerobic glycolysis rate in riboflavin/UVB‐treated PLTs was either due 1) to an impaired mitochondrial functionality leading to a decrease of oxidative phosphorylation, 2) to an alteration of the PLT storage medium characteristics (pH, oxygen content, plasma protein content), or 3) to changes in ATP consumption rate …”

“…In vitro studies reported metabolic changes, impaired mitochondrial function, accelerated passive activation, and altered agonist‐induced PLT aggregation in INTERCEPT‐treated compared to untreated PLTs . Mirasol‐treated PLTs exhibit an increased expression of P‐selectin (CD62P), higher lactate production, and increased glucose and oxygen consumption, as well as lower ATP over storage time . The Mirasol PIT leads to hyperactive PLTs resulting in a reduced degranulation capacity upon acute stimulation …”

Automated preparation of whole blood–derived platelets suspended in two different platelet additive solutions and stored for 7 days

“…Samples were first incubated with saturating concentrations of anti‐CD41a‐PerCP in the dark, without stirring, for 15 minutes at room temperature, followed by the addition of labeled conjugated MoAbs and an additional incubation for 15 minutes. Samples were then diluted with 1 mL of PBS and analyzed immediately with a flow cytometer (FACScan, Becton Dickinson, Mountain View, CA) as previously described …”

Concentrados de plaquetas procedentes de sangre total (buffy coat) u obtenidos por aféresis; ¿qué producto emplear?

“…Esta tecnología, sin embargo, podría no ser efectiva contra agentes infecciosos, fundamentalmente priones. Los estudios clínicos hasta el momento son contradictorios respecto a la efectividad clínica y hemostá tica: existen estudios que sugieren que su eficacia clínica es menor (menores CCI y mayor sangrado) 33,34 mientras que otros no observan diferencias significativas en funcionalidad in vitro o eficacia hemostá tica in vivo entre plaquetas tratadas y no tratadas 35,36 .…”