Tourism is one of the world’s most affected sectors by the impact of the COVID-19 pandemic. This article deals with the assessment of the impact of COVID-19 on the visitation of the South Moravian Region, including important cultural and natural sites, based on the analysis of empirical statistical data in the last decade and the calculation of the year-on-year change in attendance between 2019 and 2022. According to the results, the number of visitors to the South Moravian Region in 2020 fell by almost half, including a decrease of a quarter of visitors to cultural monuments compared to 2019. On the other hand, visits to natural areas with no restricted access increased by a fifth after 2020, but natural areas with restricted access fell by more than 40%. From 2021, attendance of the South Moravian Region began to increase slightly, and in 2022, it reached ninety percent of the level before 2019, including attendance at cultural and natural sites. The results of the research confirmed the growing trend in visitors to the South Moravian Region, including cultural and natural monuments, which were significantly influenced by the impact of COVID-19 on tourism after 2020, with a recovery of tourism in 2022.