Impact of Vancomycin Loading Doses and Dose Escalation on Glomerular Function and Kidney Injury Biomarkers in a Translational Rat Model

Chang, Jack; Pais, Gwendolyn; Engel, Patti L; Klimek, Patryk; Marianski, Sylwia; Valdez, Kimberly; Scheetz, Marc H.

doi:10.1128/aac.01276-22

Cited by 4 publications

(1 citation statement)

References 27 publications

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“…A modulation in the kidney functions causes an impairment in the urinary clearance of waste products from the blood which leads to an increase in the blood urea and creatinine level but Renogrit treated group showed a normal clearance rate of BUN and creatinine. Also, eGFR values decline due to Vancomycin-induced injury [43]. Interestingly, the Renogrit co-treated groups showed a dose-dependent recovery in their eGFR.…”

Section: Discussionmentioning

confidence: 95%

Renogrit attenuates Vancomycin-induced nephrotoxicity in human renal spheroids and in Sprague-Dawley rats by regulating kidney injury biomarkers and creatinine/urea clearance

Balkrishna,

Sharma,

Gohel

et al. 2023

PLoS ONE

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Vancomycin, is widely used against methicillin-resistant bacterial infections. However, Vancomycin accumulation causes nephrotoxicity which leads to an impairment in the filtration mechanisms of kidney. Traditional herbal medicines hold potential for treatment of drug-induced nephrotoxicity. Herein, we investigated protective properties of plant-based medicine Renogrit against Vancomycin-induced kidney injury. Phytometabolite analysis of Renogrit was performed by UHPLC. Spheroids formed from human proximal tubular cell (HK-2) were used for in vitro evaluation of Vancomycin-induced alterations in cell viability, P-gp functionality, NAG, KIM-1 levels, and mRNA expression of NGAL and MMP-7. The in vivo efficacy of Renogrit against Vancomycin-induced nephrotoxicity was further evaluated in Sprague-Dawley (SD) rats by measurement of BUN, serum creatinine, and their respective clearances. Moreover, eGFR, kidney-to-body weight ratio, GSH/GSSG ratio, KIM-1, NAG levels and mRNA expression of KIM-1 and osteopontin were also analyzed. Changes in histopathology of kidney and hematological parameters were also observed. Renogrit treatment led to an increase in cell viability, normalization of P-gp functionality, decrease in levels of NAG, KIM-1, and reduction in mRNA expression of NGAL and MMP-7. In Vancomycin-challenged SD rats, Renogrit treatment normalized altered kidney functions, histological, and hematological parameters. Our findings revealed that Renogrit holds a clinico-therapeutic potential for alleviating Vancomycin-associated nephrotoxicity.

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Section: Discussionmentioning

confidence: 95%