Impact of Various Parameters in Detecting Chromosomal Aberrations by FISH to Describe Radiosensitivity

Abstract

To detect a difference in chromosomal aberrations between healthy and radiosensitive individuals, the parameters "frequency of breaks per metaphase", "complex chromosomal rearrangements", and "translocations" are most suitable.

Isoform-Specific Activation of Latent Transforming Growth Factor β (LTGF-β) by Reactive Oxygen Species

“…This study found more fibrosis in patients with higher levels of in vitro induced lethal aberrations. In addition, using three color whole chromosome FISH, Keller et al found higher levels of breaks, complex chromosomal rearrangements and translocations, in in vitro irradiated lymphocytes from patients who had developed grade 3 or greater late radiation toxicity including fibrosis (118). This study; however, was based on only 5 sensitive and 11 normally responding patients.…”

Analysis of the Action of the Restriction Endonuclease AluI Using Three Different Comet Assay Protocols

“…Quite a number of techniques are intended to be used in the detection of individual radiosensitivities, such as chromosomal aberrations [8,11], 24-color FISH [14,15], micronuclei [32], DNA-PKCS and Ku (p70/p80) [3], to name just some of the approaches. As our major interest with regard to the application of the comet assay is also directed at the monitoring of individual radiosensitivities, in particular, of tumor patients who received a radiotherapy, our test was optimized to have maximum sensitivity in the dose range between 0-2 Gy and to allow the measurement of DNA repair after a single dose of 2 Gy (corresponding to the dose per fraction in conventional radiotherapy).…”

DNA Double-Strand Break Induction and Repair in Irradiated Lymphoblastoid, Fibroblast Cell Lines and White Blood Cells from ATM, NBS and Radiosensitive Patients

“…Correlations were calculated and given as Spearman's coefficient of rank correlation (rs; SPSS for Windows, Version 11.5, Munich, Germany) [12,21].…”