Impairment of the hatching process following IVF in the human and improvement of implantation by assisting hatching using micromanipulation

Cohen, Jacques; Elsner, Carlene W.; Kort, Hilton I.; Malter, Henry; Massey, J.H.; Mayer, Mary Pat; Wiemer, Klaus E.

doi:10.1093/oxfordjournals.humrep.a137044

Cited by 367 publications

(204 citation statements)

References 0 publications

Supporting

Mentioning

188

Contrasting

Unclassified

Order By: Relevance

“…Since the first assisted hatching technique that used a mechanical method, several approaches have been proposed [45], including mechanical incision of the zona [43]; chemical zona drilling with acidic medium [46]; chemical zona thinning [47]; laser-assisted hatching [33] and, more recently, piezo technology [14]. The mechanical and the chemical methods require extensive technical skill to produce uniform, well controlled and standardised micro holes using micropipettes mounted on micromanipulators.…”

Section: Techniques Of Assisted Hatchingmentioning

confidence: 99%

“…The technique of assisted hatching using partial zona dissection (PZD) to create an artificial opening of the zona pellucida of early cleaved embryos was first described by Cohen et al [46]. While the embryo is stabilised by a holding pipette, the zona pellucida is pierced with a microneedle that is pushed tangentially through the space between the zona pellucida and blastomeres until it pierces through the zona pellucida again.…”

Section: Mechanical Techniquesmentioning

confidence: 99%

“…Assisted hatching using acid Tyrode's has been described in detail by Cohen et al [15,46]. The embryo is secured on a holding pipette and a microneedle is applied to an area of the zona pellucida overlying either empty perivitelline space or extracellular fragments (i.e.…”

Section: Chemical Techniquesmentioning

confidence: 99%

“…Alternatively, blastomeres may be lost through larger holes prior to the formation of tight junctions, possibly causing monozygotic twins, mbryonic death or even vesiculation [46,80] Monozygotic twins are more common in women participating in an IVF program than in the general population. In a clinical review of 2,163 cycles of IVF, six cases of identical twin pregnancies were found [85].…”

Section: Potential Risk Of Ahmentioning

confidence: 99%

See 3 more Smart Citations

Assisted hatching in assisted reproduction: a state of the art

Hammadeh

Fischer-Hammadeh

Ali

2010

J Assist Reprod Genet

106

View full text Add to dashboard Cite

The World Health Organization estimates that one in six couples experience some delay in conception and an increasing number require treatment by the assisted conception (AC) procedures of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI).The implantation rate of embryos resulting from in vitro fertilization cycles is generally less than 20%. The exposure of oocytes and embryos to the artificial conditions of in vitro culture may have negative effects on the embryo's ability to undergo normal hatching, resulting in low rates of implantation following IVF and embryo transfer. Human embryos resulting from superovulation develop more slowly in vitro compared to embryos in vivo, manifest a relatively high degree of cytogenetic abnormalities and undergo cellular fragmentation. Artificially disrupting the zona pellucida is known as assisted hatching (AH) and there is some evidence that embryos that have undergone zona manipulation for assisted hatching tend to implant one day earlier than unhatched embryos. A variety of techniques have since been employed to assist embryo hatching, including partial mechanical zona dissection, zona drilling and zona thinning, making use of acid tyrodes, proteinases, piezon vibrator manipulators and lasers. This review will consider the impact of IVF conditions on zona pellucida physiology, zona hardening, different techniques of assisted hatching, who may benefit from assisted hatching and potential hazards.

show abstract

Section: Techniques Of Assisted Hatchingmentioning

confidence: 99%

Section: Mechanical Techniquesmentioning

confidence: 99%

Section: Chemical Techniquesmentioning

confidence: 99%

Section: Potential Risk Of Ahmentioning

confidence: 99%

See 2 more Smart Citations

Assisted hatching in assisted reproduction: a state of the art

Hammadeh

Fischer-Hammadeh

Ali

2010

J Assist Reprod Genet

106

View full text Add to dashboard Cite

show abstract

“…As part of the natural hatching process in vivo, the zona pellucida (zona) is thinned and softened by a protease-like enzyme [1,2]. In contrast, embryo culture conditions in vitro are believed to induce abnormal hardening of the zona [3], which may impair the hatching process and thus contribute to low implantation rates after embryo transfer [4].…”

Section: Introductionmentioning

confidence: 99%

Chemical zona pellucida thinning with acidified Tyrode’s solution: comparison between partial and circumferential techniques

Yano¹,

Yano²,

Kubo³

et al. 2007

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To compare effectiveness of two different chemical zona thinning techniques. Method We studied 163 patients who had experienced IVF or ICSI failures in two or more cycles. Patients were assigned to one of three groups: zona intact (n=72), partial thinning (n = 59), or circumferential thinning (n =73). Before transfer, the zonae pellucidae of embryos were thinned partially or circumferentially using acidified Tyrode's solution. Results Implantation rates were 8.9% in the intact zona group, 17.6% in the partial thinning group, and 11.9% in the circumferential thinning group: respective clinical pregnancy rates were 16.7% (12/72), 32.2% (19/59), and 27.4% (20/73). Both rates were significantly higher in the partial thinning group than the intact zona group. For circumferential thinning versus zona intact groups, differences fell short of significance. Conclusions Following embryo transfer failure, partial thinning would be recommended over circumferential thinning for successful assisted hatching.

show abstract