Impedance interrogated affinity biosensors for medical applications: novel targets and mechanistic studies

Millner, Paul A.; Caygill, Rebecca L.; Conroy, David J.R.; Shahidan, Muhammad Ashraf

doi:10.1533/9780857097187.1.103

Cited by 11 publications

(9 citation statements)

References 118 publications

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“…Then, the IME sensor was functionalised with an anti-BDNF antibody (SC-546, Santa Cruz Biotechnology) by immobilization of the antibody on the aldehyde group of the modified substrate 14 19 (see Experimental Procedures). When then samples were loaded, increases in the impedance of receptor-functionalized IMEs (impedance sensors) corresponded to the amount of the bound target molecule 20 , in this case, BDNF. The actual changes in impedance (ΔZ) were measured using an electrochemical impedance spectrometer (PGSTAT302N, Metrohm Autolab or IME analyzer, Cantis Incorporation) connected to the IME chip by two metal electrodes ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Ultra-sensitive detection of brain-derived neurotrophic factor (BDNF) in the brain of freely moving mice using an interdigitated microelectrode (IME) biosensor

Yoo

Lee

Kim

et al. 2016

Sci Rep

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Brain-derived neurotrophic factor (BDNF) plays a critical role in cognitive processes including learning and memory. However, it has been difficult to detect BDNF in the brains of behaving animals because of its extremely low concentration, i.e., at the sub-nanogram/mL level. Here, we developed an interdigitated microelectrode (IME) biosensor coated with an anti-BDNF an anti-BDNF antibody in a polydimethylsiloxane (PDMS)-based microfluidic channel chip. This sensor could detect BDNF from microliter volumes of liquid samples even at femtogram/mL concentrations with high selectivity over other growth factors. Using this biosensor, we examined whether BDNF is detectable from periodical collection of cerebrospinal fluid microdialysate, sampled every 10 min from the hippocampus of mice during the context-dependent fear-conditioning test. We found that the IME biosensor could detect a significant increase in BDNF levels after the memory task. This increase in BDNF levels was prevented by gene silencing of BDNF, indicating that the IME biosensor reliably detected BDNF in vivo. We propose that the IME biosensor provides a general-purpose probe for ultrasensitive detection of biomolecules with low abundance in the brains of behaving animals.

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Section: Resultsmentioning

confidence: 99%

Ultra-sensitive detection of brain-derived neurotrophic factor (BDNF) in the brain of freely moving mice using an interdigitated microelectrode (IME) biosensor

Yoo

Lee

Kim

et al. 2016

Sci Rep

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show abstract

“…Thus, as a label free detection, the EIS biosensors enable direct determination of biomolecular recognition actions [ 27 ]. EIS biosensors are increased significantly due to their facile manipulation, rapid response, miniaturization capability, and readiness for lab-on-a-chip integration with low cost and online measurement to detect very low concentrations [ 28 , 29 ].…”

Section: Impedimetric Biosensorsmentioning

confidence: 99%

Electrochemical Impedance Spectroscopy (EIS): Principles, Construction, and Biosensing Applications

Magar

Hassan

Mulchandani

2021

Sensors

604

238

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Electrochemical impedance spectroscopy (EIS) is a powerful technique used for the analysis of interfacial properties related to bio-recognition events occurring at the electrode surface, such as antibody–antigen recognition, substrate–enzyme interaction, or whole cell capturing. Thus, EIS could be exploited in several important biomedical diagnosis and environmental applications. However, the EIS is one of the most complex electrochemical methods, therefore, this review introduced the basic concepts and the theoretical background of the impedimetric technique along with the state of the art of the impedimetric biosensors and the impact of nanomaterials on the EIS performance. The use of nanomaterials such as nanoparticles, nanotubes, nanowires, and nanocomposites provided catalytic activity, enhanced sensing elements immobilization, promoted faster electron transfer, and increased reliability and accuracy of the reported EIS sensors. Thus, the EIS was used for the effective quantitative and qualitative detections of pathogens, DNA, cancer-associated biomarkers, etc. Through this review article, intensive literature review is provided to highlight the impact of nanomaterials on enhancing the analytical features of impedimetric biosensors.

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“…Biosensors overcome many limitations suffered by conventional analytical procedures which may be time consuming, are complex and need costly equipment [ 19 , 20 , 21 , 22 , 23 ]. Optical biosensors are considered as good alternatives to conventional analytical techniques to provide rapid, highly sensitive and real-time measurements [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Affimer-Based Europium Chelates Allow Sensitive Optical Biosensing in a Range of Human Disease Biomarkers

Al-Enezi

Vakurov

Eades

et al. 2021

Sensors

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The protein biomarker measurement has been well-established using ELISA (enzyme-linked immunosorbent assay), which offers good sensitivity and specificity, but remains slow and expensive. Certain clinical conditions, where rapid measurement or immediate confirmation of a biomarker is paramount for treatment, necessitate more rapid analysis. Biosensors offer the prospect of reagent-less, processing-free measurements at the patient’s bedside. Here, we report a platform for biosensing based on chelated Eu3+ against a range of proteins including biomarkers of cardiac injury (human myoglobin), stroke (glial fibrillary acidic protein (GFAP)), inflammation (C-reactive protein (CRP)) and colorectal cancer (carcinoembryonic antigen (CEA)). The Eu3+ ions are chelated by modified synthetic binding proteins (Affimers), which offer an alternative targeting strategy to existing antibodies. The fluorescence characteristics of the Eu3+ complex with modified Affimers against human myoglobin, GFAP, CRP and CEA were measured in human serum using λex = 395 nm, λem = 590 and 615 nm. The Eu3+-Affimer based complex allowed sensitive detection of human myoglobin, GFAP, CRP and CEA proteins as low as 100 fM in (100-fold) diluted human serum samples. The unique dependence on Eu3+ fluorescence in the visible region (590 and 615 nm) was exploited in this study to allow rapid measurement of the analyte concentration, with measurements in 2 to 3 min. These data demonstrate that the Affimer based Eu3+ complexes can function as nanobiosensors with potential analytical and diagnostic applications.

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Impedance interrogated affinity biosensors for medical applications: novel targets and mechanistic studies

Cited by 11 publications

References 118 publications

Ultra-sensitive detection of brain-derived neurotrophic factor (BDNF) in the brain of freely moving mice using an interdigitated microelectrode (IME) biosensor

Ultra-sensitive detection of brain-derived neurotrophic factor (BDNF) in the brain of freely moving mice using an interdigitated microelectrode (IME) biosensor

Electrochemical Impedance Spectroscopy (EIS): Principles, Construction, and Biosensing Applications

Affimer-Based Europium Chelates Allow Sensitive Optical Biosensing in a Range of Human Disease Biomarkers

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