Impedance spectroscopy for identifying tau protein to monitor anesthesia‐based issues

Abstract

Anesthesia-related drugs cause various side effects and health-related illnesses after surgery. In particular, neurogenerative disorder is a common problem of anesthesia-related drugs. A patient gets anesthesia as a requirement of the preoperative evaluation to diagnose the medical illness, which is caused by anesthetic drug treatment. Different blood-based biomarkers help in identifying the changes appearing in patients after anesthesia treatment. Among them, tau protein is a sensitive biomarker of neurodegenerative diseases, and the fluctuations in tau proteins are highly associated with various diseases. Furthermore, researchers have found unstable levels of tau protein after the anesthesia process. The current research has focused on quantifying tau protein via impedance spectroscopy to identify the problems caused by anesthesia-related drugs. An impedance spectroscopy electrode was modified into a multiwalled carbon nanotube, and an amine-ended aptamer was then attached. This electrode surface was used to quantify the tau protein level and reached the detection limit of 1 fM. The determination coefficient was found to be y = 369.93x + 1144.9, with R 2 = 0.9846 in the linear range of 1 fM-1 nM. Furthermore, tau protein spiked human serum was clearly identified on the immobilized aptamer surface, indicating the specific detection.

An Overview of the Latest Developments in the Electrochemical Aptasensing of Neurodegenerative Diseases

“…This biosensor has great potential and is a promising approach as a reliable analytical tool for the early diagnosis of AD. The recently reported application of MWCNTs for the highly efficient immobilization of aptamers has been described by Yin and co-workers [138]. The electrode surface was functionalized with MWCNTs via the 3-(aminopropyl) triethoxysilane (APTES) connector.…”

“…Studies on the interaction of aptamers with tau protein were performed using EIS with an LOD of 1 fM. The presented aptasensor specifically recognized tau protein in control experiments performed with (i) a complementary aptamer instead of an aptamer, The recently reported application of MWCNTs for the highly efficient immobilization of aptamers has been described by Yin and co-workers [138]. The electrode surface was functionalized with MWCNTs via the 3-(aminopropyl) triethoxysilane (APTES) connector.…”