Impedimetric analysis on the mass transfer properties of intact and competent E. coli cells

Lim, Yong Sik; Su, Chun Hao; Liao, Ying‐Chih; Lee, Sang Yup

doi:10.1016/j.bbamem.2018.10.010

Cited by 7 publications

(5 citation statements)

References 42 publications

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“…The transformation frequency of these culturable chlorineinjured bacteria increased >550-fold compared with untreated bacteria, resulting in a much higher proportion of iARG in post-disinfection water than that in pre-disinfection water [19]. Generally, competent E. coli cells are often used in bacterial transformation owing to their high permeability for bioorganic macromolecules like plasmid DNA [42]. Therefore, the mechanism for increased transformation here can be explained by the fact that the chlorine-injured bacteria also became competent, making plasmid uptake easier, due to the increased cell membrane permeability found in chlorine-injured E. coli in this study.…”

Section: Discussionmentioning

confidence: 99%

Chlorine disinfection promotes the exchange of antibiotic resistance genes across bacterial genera by natural transformation

Jin¹,

Liu²,

Da-ning³

et al. 2020

The ISME Journal

274

102

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Chlorine disinfection to drinking water plays an important role in preventing and controlling waterborne disease outbreaks globally. Nevertheless, little is known about why it enriches the antibiotic resistance genes (ARGs) in bacteria after chlorination. Here, ARGs released from killed antibiotic-resistant bacteria (ARB), and culturable chlorine-injured bacteria produced in the chlorination process as the recipient, were investigated to determine their contribution to the horizontal transfer of ARGs during disinfection treatment. We discovered Escherichia coli, Salmonella aberdeen, Pseudomonas aeruginosa and Enterococcus faecalis showed diverse resistance to sodium hypochlorite, and transferable RP4 could be released from killed sensitive donor consistently. Meanwhile, the survival of chlorine-tolerant injured bacteria with enhanced cell membrane permeabilisation and a strong oxidative stress-response demonstrated that a physiologically competent cell could be transferred by RP4 with an improved transformation frequency of up to 550 times compared with the corresponding untreated bacteria. Furthermore, the water quality factors involving chemical oxygen demand (COD Mn), ammonium nitrogen and metal ions (Ca 2+ and K +) could significantly promote above transformation frequency of released RP4 into injured E. faecalis. Our findings demonstrated that the chlorination process promoted the horizontal transfer of plasmids by natural transformation, which resulted in the exchange of ARGs across bacterial genera and the emergence of new ARB, as well as the transfer of chlorine-injured opportunistic pathogen from non-ARB to ARB. Considering that the transfer elements were quite resistant to degradation through disinfection, this situation poses a potential risk to public health.

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Section: Discussionmentioning

confidence: 99%

Chlorine disinfection promotes the exchange of antibiotic resistance genes across bacterial genera by natural transformation

Jin¹,

Liu²,

Da-ning³

et al. 2020

The ISME Journal

274

102

View full text Add to dashboard Cite

show abstract

“…Activation of the SOS response enables the uptake of eDNA by competent strains for repairing or developing antibiotic resistance [ 44 ]. Bacterial cell membrane is known as a barrier against HGT, inhibiting the transfer of eDNA into cells [ 45 , 46 ] ( Figure 4 a,b). Our previous work showed that CeO 2 NPs at low concentrations exhibited an efficient ROS scavenging capacity [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

CeO2 Nanoparticles-Regulated Plasmid Uptake and Bioavailability for Reducing Transformation of Extracellular Antibiotic Resistance Genes

Wang

et al. 2023

Nanomaterials

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The direct uptake of extracellular DNA (eDNA) via transformation facilitates the dissemination of antibiotic resistance genes (ARGs) in the environment. CeO2 nanoparticles (NPs) have potential in the regulation of conjugation-dominated ARGs propagation, whereas their effects on ARGs transformation remain largely unknown. Here, CeO2 NPs at concentrations lower than 50 mg L−1 have been applied to regulate the transformation of plasmid-borne ARGs to competent Escherichia coli (E. coli) cells. Three types of exposure systems were established to optimize the regulation efficiency. Pre-incubation of competent E. coli cells with CeO2 NPs at 0.5 mg L−1 inhibited the transformation (35.4%) by reducing the ROS content (0.9-fold) and cell membrane permeability (0.9-fold), thereby down-regulating the expression of genes related to DNA uptake and processing (bhsA, ybaV, and nfsB, 0.7–0.8 folds). Importantly, CeO2 NPs exhibited an excellent binding capacity with the plasmids, decreasing the amounts of plasmids available for cellular uptake and down-regulating the gene expression of DNA uptake (bhsA, ybaV, and recJ, 0.6–0.7 folds). Altogether, pre-exposure of plasmids with CeO2 NPs (10 and 25 mg L−1) suppressed the transformation with an efficiency of 44.5–51.6%. This study provides a nano-strategy for controlling the transformation of ARGs, improving our understanding on the mechanisms of nanomaterial-mediated ARGs propagation.

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“…The cells at competence state have higher cell membrane permeability and porosity than their non-competent counterparts, which could promoting the uptake of endogenous DNA by crossing the cell outer membrane barrier [ 22 ]. In this study, antidepressants were found to enhance the cell membrane permeability and porosity of A. baylyi ADP1.…”

Section: Resultsmentioning

confidence: 99%

“…However, antidepressants-induced cell membrane damages could not promote the transformation of comFEBC - knocked out A. baylyi by plasmid pWH1266, when the competence machinery is absent. Since ROS overgeneration is the cause of increased membrane permeability, and competent bacteria express a higher membrane permeability for biomolecules like plasmid DNA [ 5 , 22 ], antidepressants-increased membrane permeability might be the consequence of ROS-overgeneration, or the co-occurrence with the enhanced competence.…”

Section: Discussionmentioning

confidence: 99%

Antidepressants promote the spread of extracellular antibiotic resistance genes via transformation

Ding

Wang

et al. 2022

ISME Communications

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The development of antibiotic resistance as an unavoidable consequence of the application of antimicrobials is a significant concern for human health. Antidepressants are being increasingly consumed globally. Human gut microbial communities are frequently exposed to antidepressants, yet little is known about the interaction between antidepressants and antibiotic resistance. This study aimed to investigate whether antidepressants can accelerate the dissemination of antibiotic resistance by increasing the rate of the horizontal transfer of antibiotic resistance genes (ARGs). Results demonstrated that some of the commonly-prescribed antidepressants (Duloxetine, Sertraline, Fluoxetine and Bupropion) at clinically relevant concentrations can significantly (n = 9; padj < 0.01) promote the transformation of extracellular ARGs into Acinetobacter baylyi ADP1 for a maximum of 2.3-fold, which is primarily associated with the overproduction of reactive oxygen species. The increased cell membrane permeability and porosity, stimulated transcription and translation of competence, SOS response, universal stress response and ATP synthesis-related genes are also associated with antidepressants-enhanced transformation. This study demonstrated that some antidepressants can speed up the spread of antibiotic resistance by promoting the transformation of ARGs, which emphasizes the necessity to assess the potential risks of antidepressants in spreading antibiotic resistance during clinical antidepressant applications.

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Impedimetric analysis on the mass transfer properties of intact and competent E. coli cells

Cited by 7 publications

References 42 publications

Chlorine disinfection promotes the exchange of antibiotic resistance genes across bacterial genera by natural transformation

Chlorine disinfection promotes the exchange of antibiotic resistance genes across bacterial genera by natural transformation

CeO2 Nanoparticles-Regulated Plasmid Uptake and Bioavailability for Reducing Transformation of Extracellular Antibiotic Resistance Genes

Antidepressants promote the spread of extracellular antibiotic resistance genes via transformation

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