Impedimetric DNA Aptasensor for Sensitive Detection of Ochratoxin A in Food

Castillo, G. F. Torres del; Lamberti, Ilaria; Mosiello, Lucia; Hianik, Tibor

doi:10.1002/elan.201100485

Cited by 92 publications

(61 citation statements)

References 45 publications

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“…These steps add time, and this is one reason for the continued development of non-enzymatic labels and label-free devices in order to achieve portability of the devices [47,48]. These disadvantages can be also overcome with the use of aptamer nucleotide sequences and AuNPs labels that are able to discriminate between various toxin categories [42][43][44][45]. However, for the time being, such formats tend to require expensive, laboratory-based equipment that requires significant technical training to operate.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, an impedimetric DNA aptamer biosensor onto a gold electrode's surface has also been reported [45]. EIS has been used for the determination of the charge transfer resistance (R ct ) onto a gold electrode's surface, where thiolated DNA OTA aptamers of different configurations have been immobilized by chemisorption.…”

Section: Biosensors For the Detection Of Ochratoxin A In Wine Beer Amentioning

confidence: 99%

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Biosensor-Based Approaches for Detecting Ochratoxin A and 2,4,6-Trichloroanisole in Beverages

Mavrikou

Kintzios

2018

Beverages

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Mycotoxins and haloanisoles are secondary metabolites produced under special conditions of temperature and humidity by fungi colonizing a variety of commodities from preharvest up to consumer use. Ochratoxin A and 2,4,6-trichloanisole are produced mainly by species of the genus Aspergillus and Penicillium. Ochratoxin A exhibits nephrotic effects and can, potentially, be associated with human carcinogenesis, whereas 2,4,6-trichloanisole is primarily responsible for cork taint in wines. This review provides an overview of recent advances in biosensor technology for the determination of the aforementioned compounds in wine, beer and other beverages, as well as cork stoppers, which help in establishing and carrying out proper product quality-management strategies. Such a detailed investigation of biosensor-based detection methods of these toxic compounds in beverages could lead to the provision of safe-to-consume products, and allow the prioritization of future research efforts.

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Section: Discussionmentioning

confidence: 99%

Section: Biosensors For the Detection Of Ochratoxin A In Wine Beer Amentioning

confidence: 99%

Biosensor-Based Approaches for Detecting Ochratoxin A and 2,4,6-Trichloroanisole in Beverages

Mavrikou

Kintzios

2018

Beverages

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“…Among them, ferrocene [72,[83][84][85][86][87][88][89] and Ru(NH 3 ) 6 ] 3+ [75,90] were very popular, especially for the construction of impedimetric aptasensors.…”

Section: Structure-switching G4 Electrochemical Aptasensormentioning

confidence: 99%

“…The system was further improved [87], showing a detection limit comparable to that of the HPLC method (0.12 nM), and was validated in food samples. Another design for the OTA detection proposed the use of a long polyethylene glycol spacer chain, which led to the formation of long tunnels at the surface of screen-printed carbon electrodes, with aptamers acting as gates.…”

Section: Structure-switching G4 Electrochemical Aptasensormentioning

confidence: 99%

Guanine Quadruplex Electrochemical Aptasensors

Chiorcea-Paquim

Oliveira-Brett

2016

Chemosensors

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Guanine-rich nucleic acids are able to self-assemble into G-quadruplex four-stranded secondary structures, which are found at the level of telomeric regions of chromosomes, oncogene promoter sequences and other biologically-relevant regions of the genome. Due to their extraordinary stiffness and biological role, G-quadruples become relevant in areas ranging from structural biology to medicinal chemistry, supra-molecular chemistry, nanotechnology and biosensor technology. In addition to classical methodologies, such as circular dichroism, nuclear magnetic resonance or crystallography, electrochemical methods have been successfully used for the rapid detection of the conformational changes from single-strand to G-quadruplex. This review presents recent advances on the G-quadruplex electrochemical characterization and on the design and applications of G-quadruplex electrochemical biosensors, with special emphasis on the G-quadruplex aptasensors and hemin/G-quadruplex peroxidase-mimicking DNAzyme biosensors.

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“…Many different procedures have been used to improve the sensitivity of the G-quadruplex electrochemical aptasensors using amplification strategies based on redox labels, such as ferrocene [72][73][74][75][76][77][78][79][80] A c c e p t e d M a n u s c r i p t 13 transducers have been employed, such as modified platinum [89] or carbon electrodes [64,76,[89][90][91][92].…”

Section: Structure-switching G-quadruplex Electrochemical Aptasensorsmentioning

confidence: 99%

Redox behaviour of G-quadruplexes

Chiorcea-Paquim

Oliveira-Brett

2014

Electrochimica Acta

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Please cite this article as: A.-M. Chiorcea-Paquim, A.M. OliveiraBrett, Redox behaviour of G-quadruplexes, Electrochimica Acta (2013), http://dx.doi.org/10.1016/j.electacta. 2013.07.150 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Page 1 of 45A c c e p t e d M a n u s c r i p t Redox behaviour of G-quadruplexes

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Impedimetric DNA Aptasensor for Sensitive Detection of Ochratoxin A in Food

Cited by 92 publications

References 45 publications

Biosensor-Based Approaches for Detecting Ochratoxin A and 2,4,6-Trichloroanisole in Beverages

Biosensor-Based Approaches for Detecting Ochratoxin A and 2,4,6-Trichloroanisole in Beverages

Guanine Quadruplex Electrochemical Aptasensors

Redox behaviour of G-quadruplexes

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