Impedimetric genosensor for ultratrace detection of hepatitis B virus DNA in patient samples assisted by zeolites and MWCNT nano-composites

Narang, Jagriti; Singhal, Chaitali; Malhotra, Nitesh; Narang, Sumit; Pn, Anoop Krishna; Gupta, Riya; Kansal, Ruby; Pundir, C.S.

doi:10.1016/j.bios.2016.07.013

Cited by 40 publications

(17 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The crystalline structure of the used zeolite is confirmed by XRD, Figure S1A. The XRD pattern consisted of peaks indexed as the following: 9.91° (220), 11.50° (311), 16.91° (331), 22.37° (620), 25.10° (622), 28.08° (642), 30.39° (822), 32.05° (753), 34.99° (953) . EDX analysis indicated that the type of zeolite follows NaA‐type zeolites, Figure S1B .…”

Section: Resultsmentioning

confidence: 83%

Graphite/Nanocrystalline Zeolite Platform for Selective Electrochemical Determination of Hepatitis C Inhibitor Ledipasvir

et al. 2018

View full text Add to dashboard Cite

A simple and efficient procedure is described for the electrochemical determination of ledipasvir (LDP) in presence of co‐formulated drug sofosbuvir (SOF). Herein, a highly sensitive, low‐cost electrochemical protocol was utilized to fabricate a zeolite modified carbon paste electrode (ZY/CPE) through mixing of zeolite nanostructures with graphite powder. The fabricated sensor displayed high sensitivity, allowing optimal charge transfer/electrode kinetics. Different experimental and chemical factors, including electrode composition, effect of pH, scan rate and amount of ZY were evaluated carefully to obtain the highest electrochemical response. Under optimized conditions and using square wave voltammetry (SWV), the current response of the ZY/CPE electrochemical sensing platform exhibited a detection limit of 7.5×10−9 M and a large linear range from 5.0×10−8 to 1.0×10−4 M. The practical applicability of the suggested electrochemical platform was verified in the assessment of LDP in pharmaceutical formulations with excellent recoveries in the range of 99.50–98.87 %. Moreover, a biological relevance of the developed sensor was established by the analysis of LDP in human urine and plasma samples with satisfactory recoveries of 99.00 and 99.68 %, respectively. Due to the simplicity and ease of preparation of the proposed sensor, it can be used in quality control laboratories and for clinical analysis.

show abstract

Section: Resultsmentioning

confidence: 83%

Graphite/Nanocrystalline Zeolite Platform for Selective Electrochemical Determination of Hepatitis C Inhibitor Ledipasvir

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Previously several DNA biosensors have been reported involving labeling of PCR products with enzymes 17 , redox active components 18 or nanoparticles 19,20 to enhance the electrochemical signal. Nanomaterials have been used as carrier beacons for indirect; however, vigorous and precise means for detecting target molecules.…”

Section: Introductionmentioning

confidence: 99%

Detection of chikungunya virus DNA using two-dimensional MoS2 nanosheets based disposable biosensor

Singhal

Khanuja

Chaudhary

et al. 2018

Sci Rep

Self Cite

100

View full text Add to dashboard Cite

Development of platforms for a reliable, rapid, sensitive and selective detection of chikungunya virus (CHIGV) is the need of the hour in developing countries. To the best of our knowledge, there are no reports available for the electrochemical detection of CHIGVDNA. Therefore, we aim at developing a biosensor based on molybdenum disulphide nanosheets (MoS2 NSs) for the point-of-care diagnosis of CHIGV. Briefly, MoS2 NSs were synthesized by chemical route and characterized using scanning electron microscopy, transmission electron microscopy, UV-Vis spectroscopy, Raman spectroscopy and X-Ray Diffraction. MoS2 NSs were then subjected to physical adsorption onto the screen printed gold electrodes (SPGEs) and then employed for the detection of CHIGV DNA using electrochemical voltammetric techniques. Herein, the role of MoS2 NSs is to provide biocompatibility to the biological recognition element on the surface of the screen printed electrodes. The detection strategy employed herein is the ability of methylene blue to interact differentially with the guanine bases of the single and double-stranded DNA which leads to change in the magnitude of the voltammetric signal. The proposed genosensor exhibited a wide linear range of 0.1 nM to 100 µM towards the chikungunya virus DNA.

show abstract

“…The morphology of carbon materials promotes an increase in the sensitivity, selectivity, and stability of the response compared with an unmodified transducer [ 207 ]. In the work [ 208 ], multi-walled carbon nanotubes have been successfully used as an electrically conductive layer of a developed DNA sensor for determination of hepatitis B.…”

Section: Methods Of Sensors Characteristics Improvementmentioning

confidence: 99%

Sensors Based on Bio and Biomimetic Receptors in Medical Diagnostic, Environment, and Food Analysis

et al. 2018

View full text Add to dashboard Cite

Analytical chemistry is now developing mainly in two areas: automation and the creation of complexes that allow, on the one hand, for simultaneously analyzing a large number of samples without the participation of an operator, and on the other, the development of portable miniature devices for personalized medicine and the monitoring of a human habitat. The sensor devices, the great majority of which are biosensors and chemical sensors, perform the role of the latter. That last line is considered in the proposed review. Attention is paid to transducers, receptors, techniques of immobilization of the receptor layer on the transducer surface, processes of signal generation and detection, and methods for increasing sensitivity and accuracy. The features of sensors based on synthetic receptors and additional components (aptamers, molecular imprinted polymers, biomimetics) are discussed. Examples of bio- and chemical sensors’ application are given. Miniaturization paths, new power supply means, and wearable and printed sensors are described. Progress in this area opens a revolutionary era in the development of methods of on-site and in-situ monitoring, that is, paving the way from the “test-tube to the smartphone”.

show abstract

Impedimetric genosensor for ultratrace detection of hepatitis B virus DNA in patient samples assisted by zeolites and MWCNT nano-composites

Cited by 40 publications

References 31 publications

Graphite/Nanocrystalline Zeolite Platform for Selective Electrochemical Determination of Hepatitis C Inhibitor Ledipasvir

Graphite/Nanocrystalline Zeolite Platform for Selective Electrochemical Determination of Hepatitis C Inhibitor Ledipasvir

Detection of chikungunya virus DNA using two-dimensional MoS2 nanosheets based disposable biosensor

Sensors Based on Bio and Biomimetic Receptors in Medical Diagnostic, Environment, and Food Analysis

Contact Info

Product

Resources

About