Impfungen

Wendt, Sebastian; Trawinski, Henning; Pietsch, Corinna; Borte, Michael; Lübbert, Christoph

doi:10.1007/s00108-021-01193-9

Cited by 4 publications

(6 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Antibiotic usage has become a costly and risky option for managing infectious infections, as it results in the ongoing creation of antibiotic-resistant microorganisms (Edwards et al, 2021 ). On the other hand, vaccination is a more efficient and cost-effective way of preventing illnesses over a large population (Wendt et al, 2021 ). In recent times, the development of subunit vaccines made up of specific pathogenic protein sequences from bacteria has become more feasible due to the availability of detailed knowledge on the genomes and proteomes of microorganisms (Jęśkowiak et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…In recent times, the development of subunit vaccines made up of specific pathogenic protein sequences from bacteria has become more feasible due to the availability of detailed knowledge on the genomes and proteomes of microorganisms (Jęśkowiak et al, 2021 ). This has made the development of innovative and effective subunit vaccines more practical and efficient compared to traditional vaccine development methods, which have become nearly obsolete due to their poor efficacy and high costs in terms of time and money (Wendt et al, 2021 ). The results of this study demonstrate that the pcDNA3.1 (+)-cagW is an effective protected infection of H. pylori that can stimulate a robust immune response in mice.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Immunoinformatic prediction of potential immunodominant epitopes from cagW in order to investigate protection against Helicobacter pylori infection based on experimental consequences

Chehelgerdi

Heidarnia

Dehkordi

et al. 2023

Funct Integr Genomics

View full text Add to dashboard Cite

Helicobacter pylori is a leading cause of stomach cancer and peptic ulcers. Thus, identifying epitopes in H. pylori antigens is important for disease etiology, immunological surveillance, enhancing early detection tests, and developing optimal epitope-based vaccines. We used immunoinformatic and computational methods to create a potential CagW epitope candidate for H. pylori protection. The cagW gene of H. pylori was amplified and cloned into pcDNA3.1 (+) for injection into the muscles of healthy BALB/c mice to assess the impact of the DNA vaccine on interleukin levels. The results will be compared to a control group of mice that received PBS or cagW-pcDNA3.1 (+) vaccinations. An analysis of CagW protein antigens revealed 8 CTL and 7 HTL epitopes linked with AYY and GPGPG, which were enhanced by adding B-defensins to the N-terminus. The vaccine’s immunogenicity, allergenicity, and physiochemistry were validated, and its strong activation of TLRs (1, 2, 3, 4, and 10) suggests it is antigenic. An in-silico cloning and immune response model confirmed the vaccine’s expression efficiency and predicted its impact on the immune system. An immunofluorescence experiment showed stable and bioactive cagW gene expression in HDF cells after cloning the whole genome into pcDNA3.1 (+). In vivo vaccination showed that pcDNA3.1 (+)-cagW-immunized mice had stronger immune responses and a longer plasmid DNA release window than control-plasmid-immunized mice. After that, bioinformatics methods predicted, developed, and validated the three-dimensional structure. Many online services docked it with Toll-like receptors. The vaccine was refined using allergenicity, antigenicity, solubility, physicochemical properties, and molecular docking scores. Virtual-reality immune system simulations showed an impressive reaction. Codon optimization and in-silico cloning produced E. coli-expressed vaccines. This study suggests a CagW epitopes-protected H. pylori infection. These studies show that the proposed immunization may elicit particular immune responses against H. pylori , but laboratory confirmation is needed to verify its safety and immunogenicity.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Immunoinformatic prediction of potential immunodominant epitopes from cagW in order to investigate protection against Helicobacter pylori infection based on experimental consequences

Chehelgerdi

Heidarnia

Dehkordi

et al. 2023

Funct Integr Genomics

View full text Add to dashboard Cite

show abstract

“…Impfungen gehören aus Public-Health-Sicht (neben der Verfügbarkeit von sauberem Trinkwasser) zu den wichtigsten Primärpräventionsmaßnahmen und haben insbesondere auch in der Reisemedizin eine herausragende Bedeutung [1]. Es ist ärztliche Aufgabe im Rahmen der Fürsorgepflicht für einen optimalen Gesundheitsschutz bei zu betreuenden Personen zu sorgen ( § § 630 BGB).…”

Section: Rechtlichte Aspekte Beim Impfenunclassified

“…In der Beratungssituation erscheinen zur Unterstützung Aufklärungsblätter in Form von Vordrucken als sinnvoll. Auch wenn eine schriftliche Einwilligung rechtlich nicht zwingend erforderlich ist, kann diese im Rahmen rechtlicher Auseinandersetzungen entscheidenden Wert haben 1 . Bei schriftlicher Einwilligung sollten dem Impfling dann am besten Kopien über den unterzeichneten Inhalt mitgegeben werden.…”

Section: Dies and Dasunclassified