Implant Materials, Design, and Surface Topographies: Their Influence on Osseointegration of Dental Implants

Triplett, R. Gilbert; Frohberg, U; Sykaras, Nikitas; Woody, Ronald D.

doi:10.1615/jlongtermeffmedimplants.v13.i6.50

Cited by 59 publications

(71 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The consequences of this shift to the cell are not well understood, nor is it known whether integrin expression is sensitive to surface morphology or surface energy. Although ␣5␤1, which binds the RGD motif in fibronectin (45), is involved in differentiation of osteoblasts on TCPS (46), it may not play as important a role in determining cell response when cells are grown on more clinically relevant biomaterials like Ti and may promote cell attachment and proliferation at the expense of osteoblast differentiation (47).…”

mentioning

confidence: 99%

Integrin α2β1 plays a critical role in osteoblast response to micron-scale surface structure and surface energy of titanium substrates

Olivares-Navarrete

Raz

Zhao

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

215

182

View full text Add to dashboard Cite

Efforts to improve bone response to biomaterials have focused on ligands that bind ␣5␤1 integrins. However, antibodies to ␣5␤1 reduce osteoblast proliferation but do not affect differentiation when cells are grown on titanium (Ti). ␤1-silencing blocks the differentiation stimulus of Ti microtopography, suggesting that other ␤1 partners are important. Stably ␣2-silenced MG63 human osteoblast-like cells were used to test whether ␣2␤1 specifically mediates osteoblast response to Ti surface micron-scale structure and energy. WT and ␣2-silenced MG63 cells were cultured on tissue culture polystyrene (TCPS) and Ti disks with different surface microtopographies: machined pretreatment (PT) surfaces [mean peak to valley roughness (R a) < 0.02 m], PT surfaces that were grit-blasted and acid-etched (SLA; R a ‫؍‬ 4 m), and SLA with high surface energy (modSLA). Alkaline phosphatase (ALP), ␣2 and ␤1 mRNA, but not ␣5, ␣v, ␤3, type-I collagen, or osteocalcin, increased on SLA and modSLA at 6 days. ␣2 increased at 8 days on TCPS and PT, but remained unchanged on SLA and modSLA. ␣2-protein was reduced 70% in ␣2-siRNA cells, whereas ␣5-mRNA and protein were unaffected. ␣2-knockdown blocked surface-dependent increases in ␤1 and osteocalcin and decreases in cell number and increases in ALP and local factors typical of MG63 cells grown on SLA and modSLA [e.g., prostaglandin E 2, osteoprotegerin, latent and active TGF-␤1, and stimulatory effects of 1␣,25(OH)2D3 on these parameters]. This finding indicates that ␣2␤1 signaling is required for osteoblastic differentiation caused by Ti microstructure and surface energy, suggesting that conclusions based on cell behavior on TCPS are not predictive of behavior on other substrates or the mechanisms involved.␣-2 integrin siRNA ͉ MG63 human osteoblasts ͉ titanium surface roughness T itanium (Ti) and Ti alloys are commonly used as biomaterials because their surface properties provide a biocompatible interface with peri-implant tissues. Strategies for modifying the nature of this interface frequently involve changes to the surface, thereby affecting protein adsorption, cell-substrate interactions, and tissue development (1). A common modification has been to create micron-scale and submicron scale roughness. Preclinical and clinical studies (2-12) show that these surfaces support greater bone-to-implant contact than smooth surfaces.How surface microstructure promotes an osteogenic response is an important question, because bone-forming osteoblasts preferentially colonize bone surfaces that have been preconditioned by bone-resorbing osteoclasts (13), resulting in complex micron-scale and submicron-scale morphologies (14). In vitro experiments using model surfaces indicate that migration, growth, and colony morphology of rat bone marrow cells (15) and osteoblasts (16-18) are sensitive to microstructure. These observations suggest that structural elements can modulate the spatial organization of cells and their ECM.The topography of osteoclast resorption pits in bone can be modeled by using Ti subs...

show abstract

mentioning

confidence: 99%

Integrin α2β1 plays a critical role in osteoblast response to micron-scale surface structure and surface energy of titanium substrates

Olivares-Navarrete

Raz

Zhao

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

215

182

View full text Add to dashboard Cite

show abstract

“…Using his work as a foundation, other scientists have tried to innovate dental implants by investigating the use of new implant materials, new implant designs, new external treatments, and novel surgical techniques [6,7]. Many also attempted to understand patient's systemic conditions, thereby hoping to reduce osseointegration time and extend the clinical application of implants without reducing the benchmarked success rate of the Bränemark protocols.…”

Section: Summary Of Pertinent Researchmentioning

confidence: 99%

Current knowledge of prosthetic rehabilitation using implants: a literature review

Silva¹,

Ribeiro²,

Malaquias³

et al. 2017

Clin Med Invest

View full text Add to dashboard Cite

This study proposes a brief literature review on the current condition of implant dentistry with regard to rehabilitation of prosthetic osseo integrated implants. At this stage, manufacturers of implants and prosthetic components have provided a number of innovative options for dental surgeons with promises of high performance and predictability. However, it is known that the established concepts and biological conditions such asosseo integration parameters, surface treatment, implant design, and early and immediate loading are difficult to be changed. This scientific basis is increasingly necessary for the update of professionals who are active in the academic or clinical areas in order to provide the best quality of treatment to patients and consequent quality of life.

show abstract

“…The low density, high strength, durability and resistance to corrosion make titanium the material of choice for dental implants. One of the main purposes of research in the implant field was to improve the macroscopic and microscopic properties of the implant surface to achieve better treatment outcomes and long-term survival of osseointegrated implants [3][4][5] . The implant surface/bone interface was considered a key determinant in the implant treatment success.…”

Section: Introductionmentioning

confidence: 99%

Osteoblastic differentiating potential of dental pulp stem cells <i>in vitro</i> cultured on a chemically modified microrough titanium surface

et al. 2018

View full text Add to dashboard Cite

Titanium surface modification is critical for dental implant success. Our aim was to determine surfaces influence on dental pulp stem cells (DPSCs) viability and differentiation. Implants were divided into sandblasted/acid-etched (control) and sandblasted/acid-etched coated with calcium and magnesium ions (CaMg), supplied as composite, (test). Proliferation was evaluated by MTT, differentiation checking osteoblastic gene expression, PGE2 secretion and matrix formation, inflammation by Interleukin 6 (IL-6) detection. MTT and IL-6 do not modify on test. A PGE2 increase on test is recorded. BMP2 is higher on test at early experimental points, Osterix and RUNX2 augment later. Alizarin-red S reveals higher matrix production on test. These results suggest that test surface is more osteoinductive, representing a start point for in vivo studies aiming at the construction of more biocompatible dental implants, whose integration and clinical performance are improved and some undesired effects, such as implant stability loss and further surgical procedures, are reduced.

show abstract

Implant Materials, Design, and Surface Topographies: Their Influence on Osseointegration of Dental Implants

Cited by 59 publications

References 0 publications

Integrin α2β1 plays a critical role in osteoblast response to micron-scale surface structure and surface energy of titanium substrates

Integrin α2β1 plays a critical role in osteoblast response to micron-scale surface structure and surface energy of titanium substrates

Current knowledge of prosthetic rehabilitation using implants: a literature review

Osteoblastic differentiating potential of dental pulp stem cells <i>in vitro</i> cultured on a chemically modified microrough titanium surface

Contact Info

Product

Resources

About