Implant recognition and gender expression following ampoule-androgenic gland implantation in Litopenaeus vannamei females (Penaeidae)

Vega-Alpízar, Jose Luis; Alfaro-Montoya, Jorge; Hernández-Noguera, Luís; Umaña-Castro, Rodolfo; Aflalo, Eliahu D.; Sagi, Amir

doi:10.1016/j.aquaculture.2016.11.007

Cited by 7 publications

(6 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This also explains why the MniDMRT11E mRNA was visualized in glandular cells of AG (Figure 8). Considering the important involvement of IAG in crustacean males [4,5,6,7,8,36] and the vitally important involvement of VG in females [32,38,40], we hypothesized that MniDMRT11E may participate in this pathway, either directly or indirect upstream of IAG / VG . This result provides strong evidence for an important role of MniDMRT11E in promoting ovary maturity and inhibiting testis maturity.…”

Section: Discussionmentioning

confidence: 99%

“…At present, there are limited studies, and most species-related studies are still lacking. Insulin-like androgenic gland factor ( IAG ) is a unique gene in crustaceans that is specifically expressed in the androgenic gland (AG), and also plays an important role in sex differentiation, but the specific molecular mechanism is not yet clear [4,5,6,7,8].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification and Characterization of the DMRT11E Gene in the Oriental River Prawn Macrobrachium nipponense

Wang

Jin

et al. 2019

IJMS

View full text Add to dashboard Cite

The doublesex and mab-3 related transcription factor (DMRT) gene family involvement in sex development is widely conserved from invertebrates to humans. In this study, we identified a DM (Doublesex/Mab-3)-domain gene in Macrobrachium nipponense, which we named MniDMRT11E because it has many similarities to and phylogenetically close relationships with the arthropod DMRT11E. Amino acid alignments and structural prediction uncovered conservation and putative active sites of the DM domain. Real-time PCR analysis showed that the MniDMRT11E was highly expressed in the ovary and testis in both males and females. Cellular localization analysis showed that DMRT11E was mainly located in the oocytes of the ovary and the spermatocyte of the testis. During embryogenesis, the expression level of MniDMRT11E was higher at the cleavage stage than at other stages. During the different stages of ovarian development, MniDMRT11E expression gradually increased from OI to OIII and decreased to the lowest level at the end of OIV. The results indicated that MniDMRT11E probably played important roles in embryonic development and sex maturity in M. nipponense. MniDMRT11E dsRNA injection also significantly reduced vitellogenin (VG) expression and significantly increased insulin-like androgenic gland factor (IAG) expression, indicating a close relationship in gonad development.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of the DMRT11E Gene in the Oriental River Prawn Macrobrachium nipponense

Wang

Jin

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…At present, studies in the sex determination mechanism of crustaceans are limited, and most species-related studies are still undefined. Insulin-like androgenic gland factor (IAG), a unique gene in crustaceans, plays an important role in sex differentiation, but its specific molecular mechanism is not yet clear (Ventura et al 2008;Chung et al 2011;Ma et al 2013;Vega-Alpízar et al 2017;Guo et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning, expression pattern analysis, and in situ hybridization of a Transformer-2 gene in the oriental freshwater prawn, Macrobrachium nipponense (de Haan, 1849)

Wang

Jin

et al. 2019

3 Biotech

View full text Add to dashboard Cite

In this study, we isolated a full-length cDNA sequence from Macrobrachium nipponense and investigated its gene function. We named the gene Mntra-2a because of high similarities and close evolutionary divergence with arthropod tra-2. The fulllength cDNA of Mntra-2a was 1293 bp, consisting of a 212 bp 5′ UTR, a 268 bp 3′ UTR, and an ORF of 813 bp encoding 270 amino acids. It contained an RNA recognition motif and a linker region. Real-time PCR analysis showed that Mntra-2a was highly expressed in the gonads of both males and females. Further in situ hybridization analysis showed that Mntra-2a was mainly located in oocytes and spermatocytes. During embryogenesis, Mntra-2a expression was higher in the cleavage and nauplius stages. During the ovarian reproductive cycle, Mntra-2a expression reached a peak at OvaryV and decreased to the lowest level at OvaryIV. These results indicated that Mntra-2a probably played important roles in embryonic development and early gonad development in M. nipponense. Our results provide basic information for further functional studies of tra-2 in M. nipponense.

show abstract

“…That study also served to highlight the IAG-switch as a pivotal sex differentiating mechanism in crustaceans, since females implanted with sperm duct or testicular tissue were not masculinized, while ∼80% of AG-implanted females showed some degree of masculinization (84). In another important aquaculture species, the penaeid Pacific white shrimp Litopenaeus vannamei, AG ablation of males in various post-larval stages resulted in inferior development of the AMs and degradation of the spermatids in the gonads (98), while AG implanted females did not develop AMs and only the minority developed male-like claspers on the first endopods (99). However, we note that complete and functional sex reversal in this important species has not yet been achieved, despite the extensive attempts of various research groups around the world.…”

Section: The Iag-switch-a Master Sex Controlling Device In Crustaceansmentioning

confidence: 99%

The “IAG-Switch”—A Key Controlling Element in Decapod Crustacean Sex Differentiation

Levy

Sagi

2020

Front. Endocrinol.

Self Cite

View full text Add to dashboard Cite

Implant recognition and gender expression following ampoule-androgenic gland implantation in Litopenaeus vannamei females (Penaeidae)

Cited by 7 publications

References 43 publications

Identification and Characterization of the DMRT11E Gene in the Oriental River Prawn Macrobrachium nipponense

Identification and Characterization of the DMRT11E Gene in the Oriental River Prawn Macrobrachium nipponense

Molecular cloning, expression pattern analysis, and in situ hybridization of a Transformer-2 gene in the oriental freshwater prawn, Macrobrachium nipponense (de Haan, 1849)

The “IAG-Switch”—A Key Controlling Element in Decapod Crustacean Sex Differentiation

Contact Info

Product

Resources

About