Implant Soft-Tissue Attachment Using 3D Oral Mucosal Models—A Pilot Study

Barker, Emilia; AlQobaly, Lina; Shaikh, Zahab N; Franklin, Kirsty; Moharamzadeh, Keyvan

doi:10.3390/dj8030072

Cited by 18 publications

(19 citation statements)

References 51 publications

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“…In all three-dimensional oral mucosal model systems, the keratinocytes exposed to air formed a stratified structure, consisting of several thick cell layers. In most studies, the models were exposed in ALI from 6 days [ 46 , 47 ], 10 days [ 48 ], and more than 14 days [ 49 , 50 ]. However, the models developed in our study were increased to ALI for only up to 4 days.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, in our study, the pull tests were performed 14 days after disk insertion, and period of ALI was only up to 4 days, therefore the resultant models have minimum thickness of epithelial layers, thus it may impact the permeability of the model and the specimen–soft tissue interface. Our 3D-PIMM also lacked normal oral cavity environmental conditions, such as the presence of bacterial products that can affect soft-tissue attachment during the inflammatory healing phase [ 46 ].…”

Section: Strengths and Limitations Of The Studymentioning

confidence: 99%

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An In-Vitro Analysis of Peri-Implant Mucosal Seal Following Photofunctionalization of Zirconia Abutment Materials

et al. 2021

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The presence of epithelial and connective tissue attachment at the peri-implant–soft tissue region has been demonstrated to provide a biological barrier of the alveolar bone from the oral environment. This barrier can be improved via surface modification of implant abutment materials. The effect of photofunctionalization on creating a bioactive surface for the enhancement of the epithelial and connective tissue attachment of zirconia implant abutment’s peri-implant mucosal interface using organotypic model has not been investigated. Therefore, this study aimed to evaluate the soft tissue seal around peri-implant mucosa and to understand the effect of photofunctionalization on the abutment materials. Three types of abutment materials were used in this study; yttria-stabilized zirconia (YSZ), alumina-toughened zirconia, and grade 2 commercially pure titanium (CPTi) which were divided into nontreated (N-Tx) and photofunctionalized group (UV-Tx). The three-dimensional peri-implant mucosal model was constructed using primary human gingival keratinocytes and fibroblasts co-cultured on the acellular dermal membrane. The biological seal was determined through the concentration of tritiated water permeating the material–soft tissue interface. The biological seal formed by the soft tissue in the N-Tx group was significantly reduced compared to the UV-treated group (p < 0.001), with YSZ exhibiting the lowest permeability among all materials. Photofunctionalization of implant abutment materials improved the biological seal of the surrounding soft tissue peri-implant interface.

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Section: Discussionmentioning

confidence: 99%

Section: Strengths and Limitations Of The Studymentioning

confidence: 99%

An In-Vitro Analysis of Peri-Implant Mucosal Seal Following Photofunctionalization of Zirconia Abutment Materials

et al. 2021

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“…The development of TE may potentially contribute to a better understanding of the interactions that take place between the microorganisms and the resident cells of the periodontal/peri-implant soft tissues [141,142]. Such an organotypic in vitro model that mimics the tooth-/implant-epithelial attachment is, however, lacking at present and these advancements in research models may shed light on various aspects of periodontal and peri-implant diseases, ultimately leading to the design of more effective treatment strategies [143].…”

Section: Future Directions: 3d Culture Models To Study the Periodonta...mentioning

confidence: 99%

Microbial–stem cell interactions in periodontal disease

Iliopoulos

Layrolle

Apatzidou

2022

Journal of Medical Microbiology

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Periodontitis is initiated by hyper-inflammatory responses in the periodontal tissues that generate dysbiotic ecological changes within the microbial communities. As a result, supportive tissues of the tooth are damaged and periodontal attachment is lost. Gingival recession, formation of periodontal pockets with the presence of bleeding, and often suppuration and/or tooth mobility are evident upon clinical examination. These changes may ultimately lead to tooth loss. Mesenchymal stem cells (MSCs) are implicated in controlling periodontal disease progression and have been shown to play a key role in periodontal tissue homeostasis and regeneration. Evidence shows that MSCs interact with subgingival microorganisms and their by-products and modulate the activity of immune cells by either paracrine mechanisms or direct cell-to-cell contact. The aim of this review is to reveal the interactions that take place between microbes and in particular periodontal pathogens and MSCs in order to understand the factors and mechanisms that modulate the regenerative capacity of periodontal tissues and the ability of the host to defend against putative pathogens. The clinical implications of these interactions in terms of anti-inflammatory and paracrine responses of MSCs, anti-microbial properties and alterations in function including their regenerative potential are critically discussed based on literature findings. In addition, future directions to design periodontal research models and study ex vivo the microbial–stem cell interactions are introduced.

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“…DMEM/F-12, supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 µg/mL streptomycin is used for the culture of a wider spectrum of cells, including fibroblasts, neurons, muscle cells, and cell lines including HeLa [22]. DMEM/F-12 with 10% FBS has less frequently been used to culture OKF6/TERT-2 cells [23,24]. Most other studies have used the KSFM methodology as described by Dickson et al [1] OKF6/TERT-2 cells were originally established in KSFM with its own set of nutrients, but DMEM/F12, which consists of a different set of nutrients, has also been used.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Culture Media for <em>in-vitro</em> Expansion of Oral Epithelial Keratinocytes: Implications for Testing E-liquid Flavors

Cuadra¹,

Shamim²,

Shah³

et al. 2023

Preprint

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Background: Understanding in vitro expansion of OKF6/TERT-2 oral epithelial cells is important for studying molecular biology of disease and pathology affecting the oral cavity. The media used for any cell culture is paramount in terms of efficient output. Therefore, this study aims to compare two different media for OKF6/TERT-2 cultures: Keratinocyte Serum-Free Medium (KSFM) and a composite medium comprised of DMEM/F-12 mixed with KSFM (referred to as DFK). For application purposes, this investigation also compares the toxicological effects of flavored electronic cigarette liquids (E-liquids) on OKF6/TERT-2 cultures grown in both media. Methods: Cells were grown in KSFM and DFK media and cellular growth, morphology, gene expression of mucins and tight junctions, as well as wound-healing were determined. Additionally, cellular viability and cytotoxicity were indexed after E-liquid exposures. Results: While overall cellular morphologies remained unaltered, cells grown in DFK reached confluency faster. Except for claudin-1, there is no appreciable difference in expression of the other genes tested. Additionally, cultures in DFK appear more sensitive to E-liquids ± flavors. Conclusions: DFK is an alternative medium for cultivation of OKF6/TERT-2 cells to study molecular biology of disease and pathology, such as their responses to E-liquids ± flavors.

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Implant Soft-Tissue Attachment Using 3D Oral Mucosal Models—A Pilot Study

Cited by 18 publications

References 51 publications

An In-Vitro Analysis of Peri-Implant Mucosal Seal Following Photofunctionalization of Zirconia Abutment Materials

An In-Vitro Analysis of Peri-Implant Mucosal Seal Following Photofunctionalization of Zirconia Abutment Materials

Microbial–stem cell interactions in periodontal disease

Comparison of Culture Media for <em>in-vitro</em> Expansion of Oral Epithelial Keratinocytes: Implications for Testing E-liquid Flavors

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