Implantation of Cultured Preadipocyte Using Chitosan/Alginate Sponge

Rhie, Jong Won; Song, Jiankang; Lee, Paik Kwon; Ahn, Sang Tae

doi:10.4028/0-87849-436-7.349

Cited by 1 publication

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“…It was previously reported that implantable 3D scaffolds made of PLGA, hyaluronic acid, and chitosan were seeded with preadipocytes to form engineered adipose tissues in vivo. [9][10][11] For soft tissue regeneration, however, injectable scaffold materials that can be administered using a syringe are highly favorable over implantable scaffold materials for cosmetic purposes to prevent scar formation by surgical procedures.…”

Section: Introductionmentioning

confidence: 99%

Injectable Cellular Aggregates Prepared from Biodegradable Porous Microspheres for Adipose Tissue Engineering

Chung

Park

2009

Tissue Engineering Part A

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Cellular aggregates were prepared using biodegradable porous microspheres for injectable reconstruction of soft tissues in vivo. Biodegradable porous microspheres with sizes of approximately 50 microm were prepared by a porogen leaching-phase separation process in an oil-in-water single-emulsion method using poly(D,L-lactide-co-glycolide). 3T3 L1 mouse preadipocyte cells were transformed into cellular aggregates by suspension cultivation in a spinner flask using the porous microspheres as effective buoyant and cell-adhesive microcarriers. Spherically shaped aggregates were readily formed with sizes of up to 1000 microm with a higher number of viable cells compared to the nonporous microspheres. The resultant cellular aggregates exhibited far better extent of differentiation into adipocytes than the cell aggregates prepared from nonporous microspheres or monolayer cultured cells. The cellular aggregates could also be injected into nude mice using a syringe needle for adipose tissue regeneration in vivo. Immunohistochemical studies showed that the aggregate-derived tissues with porous microspheres displayed histological characteristics similar to adipose tissues 4 weeks after injection.

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Section: Introductionmentioning

confidence: 99%