This work presents the performance of a Dual-band patch antenna with L-shaped slot for biomedical applications. The antenna works at 2.4 GHz and 3.33 GHz. A semi-flexible substrate material which is “Taconic TLX (tm)” with a relative dielectric constant, ℇr of 2.55, loss tangent, tan δ of 0.0019 and thickness, h of 0.7 mm has been proposed to be the isolant element of the antenna. The size of the antenna is 60 x 55 mm². The slots in the rectangular radiating patch were introduced to produce the resonant frequencies of 2.4GHz and 3.33GHz. The simulations of the microstrip patch antenna shows the reflection coefficient, S11 of -31.67 dB and -20.25 dB at 2.4 GHz and 3.27 GHz respectively. The proposed antenna shows a peak gain of 5.01 dB at 2.4 GHz. The results were obtained using HFSS (high frequency structured simulator) software.