Implementation of a Lipase A spore display in Paenibacillus polymyxa.

Abstract

This study demonstrates the use of a spore display in Paenibacillus polymyxa for the first time, specifically incorporating fluorescent spores expressing the green fluorescent protein (GFP) and the functional display of the lipase A (LipA). Spore display allows the presentation of heterologous proteins on the surface of bacterial spores, offering numerous advantages in various biotechnological applications. The successful implementation of spore display in P. polymyxa involved engineering the spore surface to produce GFP fused to an anchoring protein in the crust, resulting in fluorescent spores that could be readily visualized. After this initial proof-of-concept, LipA from P. polymyxa was heterologously expressed and displayed on the spore surface. The lipase activity was measured over a wide temperature range and an increase in activity up to 70°C was observed. The immobilized catalyst was recycled using simple centrifugation seven times without significant loss of activity.