Implementation of conifer somatic embryogenesis in clonal forestry:
 technical
requirements and deployment considerations

Park, Yill-Sung

doi:10.1051/forest:2002051

Cited by 157 publications

(117 citation statements)

References 16 publications

Supporting

Mentioning

100

Contrasting

Unclassified

Order By: Relevance

“…But, more importantly, vegetative propagation is a very effective way to capture the best genetic stock from the breeding program. Compared to horticultural cuttings usually subjected to decreased rooting with ageing, in vitro biotechnology is considered to have greater potential for sustained clonal propagation at lower cost, especially the most powerful somatic embryogenesis technique coupled with long-term frozen storage to preserve juvenility [34,42,45]. Since our initial work [3], significant improvement of protocols from initiation of embryonal-suspensor masses (ESM) to maturation of somatic embryos have been obtained [28,36,37].…”

Section: Introductionmentioning

confidence: 99%

Towards genetic engineering of maritime pine (Pinus pinaster Ait.)

Trontin¹,

Harvengt²,

Garin³

et al. 2002

Ann. For. Sci.

View full text Add to dashboard Cite

-Using our improved protocols for somatic embryogenesis in Pinus pinaster, transgenic tissues and plantlets were recovered after microprojectile bombardment (biolistic) or cocultivation of embryonal-suspensor masses (ESM) with Agrobacterium tumefaciens. Transformation experiments were carried out with selectable hpt gene (hygromycin B resistance) and reporter gus gene (β-glucuronidase activity). With both methods, hygromycin was shown to be an effective selective agent of transformed cells within 4-19 weeks. The mean number of hygromycin-resistant lines expressing gus per gram ESM subjected to DNA transfer, ranged from 7.0 to 8.5 using biolistic and 0 to 67.3 during Agrobacterium experiments. Mature somatic embryos obtained from some transformed lines were converted into plantlets and grown in the greenhouse. The whole process (from transformation to plant acclimatisation) could be completed within only 12 months. The transgenic state of ESM, somatic embryos and plants was confirmed by histochemical GUS assays and molecular methods.

show abstract

Section: Introductionmentioning

confidence: 99%

Towards genetic engineering of maritime pine (Pinus pinaster Ait.)

Trontin¹,

Harvengt²,

Garin³

et al. 2002

Ann. For. Sci.

View full text Add to dashboard Cite

show abstract

“…Nevertheless, its long life span and irregular seed crops, the difficulty of seed conservation and of vegetative reproduction are serious drawbacks for the use of the classical genetic improvement programs in this species. Somatic embryogenesis has been tested for largescale plant propagation of a range of tree species (Bueno et al, 1992;Hernandez et al, 2003a;Lelu-Walter and Pâques, 2009;Manzanera et al, 1993;Park, 2002;Sutton, 2002). Nevertheless, embryo dormancy and immaturity problems needed to be overcome.…”

Section: Introductionmentioning

confidence: 99%

Oak somatic and gametic embryos maturation is affected by charcoal and specific aminoacids mixture

Pintos¹,

Manzanera

Bueno³

2010

Ann. For. Sci.

View full text Add to dashboard Cite

Abstract• Development of both somatic and gametic embryogenesis has many applications in clonal forestry and genetic improvement, for instance as mass-propagation of genetically improved plants and production of pure lines through doubled-haploid plant regeneration from gametic embryos.• The goal of this work was to improve growth, maturation and plantlet regeneration of cork oak (Quercus suber L.) embryos from both somatic and gametic origin.• Activated charcoal promoted a significant increase in growth in terms of relative size and weight of both somatic and doubled-haploid embryos, as well as a more efficient control of secondary somatic embryogenesis during development. A significant interaction was also observed with amino acid nutrition. While some amino acids (i.e., glutamine, arginine or asparagine) did not show significant differences with the controls, a mixture of these three amino acids or gamma amino butyric acid stimulated embryo growth. The highest survival rate during acclimation of plantlets from both somatic and doubled-haploid origin was obtained when the embryos had been previously cultured on basal medium with 3% sucrose and 1% activated charcoal for two months at 4 • C and germinated on medium supplemented with 6-benzylaminopurine and indole-3-butyric acid.• We obtained more than 900 cork oak plantlets acclimated from several embryogenic lines, with a high survival rate, demonstrating that this methodology is applicable for large scale plantlet production. We also report the first regeneration of doubled-haploid plantlets in cork oak. • Le développement des deux embryogenèses gamétique et somatique a de nombreuses applications dans la foresterie clonale et l'amélioration génétique, par exemple en tant que propagation en masse de plants génétiquement améliorés et la production de lignées pures par régénération de plants haploïdes doublés d'embryons gamétiques. Mots-clés• L'objectif de ce travail était d'améliorer la croissance, la maturation et la régénération des plantules des embryons de chêne-liège (Quercus suber L.) des deux origines gamétique et somatique.• Le charbon actif a favorisé une augmentation significative de la croissance en termes de taille et de poids des embryons haploïdes doublés et somatiques, ainsi qu'un contrôle plus efficace de l'embryogenèse somatique secondaire au cours du développement. Une interaction significative a également été observée avec la nutrition aminoacide. Alors que certains acides aminés (c'est-à-dire, la glutamine, l'arginine ou l'asparagine) ne montrent pas de différences significatives avec les témoins, un mélange de ces trois acides aminés ou l'acide butyrique amino gamma a stimulé la croissance de l'embryon. Le taux de survie le plus élevé au cours de l'acclimatation des plantules d'origine à la fois somatique et haploïde doublé, a été obtenu lorsque les embryons ont été auparavant cultivés sur milieu de base à 3 % de saccharose et de 1 % de charbon actif pendant deux mois à 4 • C et ont germé sur un milieu complété par la 6-benzylaminopurine et ...

show abstract

“…Conifer SE has been demonstrated for many genera (Park 2002;Sutton 2002;Tautorus et al 1991). It is a multistage process, which proceeds through initiation, proliferation, maturation and germination.…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis in mature zygotic embryos of Picea likiangensis

Chen

Fang

et al. 2010

Biologia

View full text Add to dashboard Cite

Somatic embryogenesis (SE) was successfully induced from mature zygotic embryos of seven families of Picea likiangensis (Franch.) Pritz after 20 weeks culture on initiation medium. Three basal media (one-half strength LM medium, one-half strength LP medium and improved LP medium) with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (6-BA) were tested but only one-half strength LM medium supplemented with 2,4-D and 6-BA was successful for the embryogenic cultures (EC) initiation. The initiation frequencies of EC varied greatly from different families when culturing on the same initiation medium. The highest frequency (41.3%) was induced from one of the families on one-half strength LM medium supplemented with 3 mg L −1 2,4-D and 1.5 mg L −1 6-BA and 16.83% on average for seven families. EC were subcultured and proliferated on the same medium as the initiation one every 10 days. 3 lines of EC induced from the same family were applied in maturation experiment. Cotyledonary somatic embryos were observed after EC were transferred to maturation media of one-half strength LM medium containing 20-80 mg L −1 abscisic acid and 7.5% polyethylene glycol (PEG-4000). However, one-half strength LM medium supplemented with 40 mg L −1 or 60 mg L −1 ABA and 7.5% PEG gave the best maturation and the 3 lines showed different ability in maturation. Over 80% cotyledonary somatic embryos germinated normally on DCR medium containing 0.2% activated carbon. The success on SE induction of the species has provided an effective clonal propagation method for this important tree's genetic improvement.

show abstract

Implementation of conifer somatic embryogenesis in clonal forestry: technical requirements and deployment considerations

Cited by 157 publications

References 16 publications

Towards genetic engineering of maritime pine (Pinus pinaster Ait.)

Towards genetic engineering of maritime pine (Pinus pinaster Ait.)

Oak somatic and gametic embryos maturation is affected by charcoal and specific aminoacids mixture

Somatic embryogenesis in mature zygotic embryos of Picea likiangensis

Contact Info

Product

Resources

About