2024
DOI: 10.1093/biomethods/bpae042
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Implementation of plate reader-based indooxine and Nessler protocols for monitoring L-asparaginase serum activity in childhood acute lymphoblastic leukaemia

Bozhidar Vergov,
Yordan Sbirkov,
Danail Minchev
et al.

Abstract: Background Monitoring the blood serum activity of L-asparaginase in children with acute lymphoblastic leukemia (ALL) has been highly recommended to detect enzyme inactivation that can cause relapse and to avoid unwanted toxicity. Nevertheless, perhaps at least partially due to the lack of clinically approved commercially available kits or standardised and independently reproduced and validated in-house protocols, laboratory assay-based determination of the optimal doses of L-asparaginase is n… Show more

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